Freshly generated stainless steel welding fume induces greater lung inflammation in rats as compared to aged fume

Antonini, James M.; Clarke, Robert W.; Murthy, G. G. Krishna; Sreekanthan, Pradeep; Jenkins, N; Eagar, T. W.; Brain, Joseph D.

doi:10.1016/s0378-4274(98)00103-9

Cited by 71 publications

(49 citation statements)

References 27 publications

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“…The generation of smoke at the workplace when the PS board is heated and dried may indicate the formation of a condensation aerosol (containing nanoparticles) from volatilised material. Particularly, as the freshly generated fumes are highly reactive and damaging [20], the nanoparticles entered the body while the spraying, heating and drying of the boards were being performed may exert great toxicities on the exposure workers. In addition, this could help to explain the nanoparticles deposition in the respiratory tract and in BALF, formation of lung fibrosis, occurrence of multi-serous membrane fluid due to the irritation of nanoparticles to cell membrane, existence of foreign body granulomas in pleura, inflammatory infiltration and hilar lymphadenopathy.…”

Section: Inhalationmentioning

confidence: 99%

Exposure to nanoparticles is related to pleural effusion, pulmonary fibrosis and granuloma

Song¹,

Li²,

Du³

2009

European Respiratory Journal

381

206

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Nano materials generate great benefits as well as new potential risks. Animal studies and in vitro experiments show that nanoparticles can result in lung damage and other toxicity, but no reports on the clinical toxicity in humans due to nanoparticles have yet been made.The present study aimed to examine the relationship between a group of workers' presenting with mysterious symptomatic findings and their nanoparticle exposure.Seven young female workers (aged 18-47 yrs), exposed to nanoparticles for 5-13 months, all with shortness of breath and pleural effusions were admitted to hospital. Immunological tests, examinations of bacteriology, virology and tumour markers, bronchoscopy, internal thoracoscopy and video-assisted thoracic surgery were performed. Surveys of the workplace, clinical observations and examinations of the patients were conducted.Polyacrylate, consisting of nanoparticles, was confirmed in the workplace. Pathological examinations of patients' lung tissue displayed nonspecific pulmonary inflammation, pulmonary fibrosis and foreign-body granulomas of pleura. Using transmission electron microscopy, nanoparticles were observed to lodge in the cytoplasm and caryoplasm of pulmonary epithelial and mesothelial cells, but are also located in the chest fluid. These cases arouse concern that long-term exposure to some nanoparticles without protective measures may be related to serious damage to human lungs.

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Section: Inhalationmentioning

confidence: 99%

Exposure to nanoparticles is related to pleural effusion, pulmonary fibrosis and granuloma

Song¹,

Li²,

Du³

2009

European Respiratory Journal

381

206

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“…Thus, its lack of specificity toward reactive oxygen species (LeBel et al 1992), coupled with its positive response to all the oxidants constituting "ROS" have made it an appealing probe for studying toxicological phenomena, and has led to its wide use in biological systems for detecting oxidative activity and indicating oxidative stress. Recently, DCFH has also been used in the assay of particles for ROS formed from welding fumes (Antonini et al 1998). …”

Section: Reagentsmentioning

confidence: 99%

Characterization of Wintertime Reactive Oxygen Species Concentrations in Flushing, New York

Venkatachari

Hopke

Brune

et al. 2007

Aerosol Science and Technology

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One of the main hypotheses for the species causing the observed health effects of ambient particulate matter is peroxides and other reactive oxygen species (ROS). However, there is currently very little data available on the concentrations of particle-bound ROS or their behavior in different physical locations and seasons. The concentrations of particle-bound ROS were determined for various size fractions of the aerosol, ranging from 10 nm to 18 μm, in Flushing, New York during the period of January and early February 2004. Sampling was carried out at 3-hour intervals using a MOUDI TM cascade impactor. The collected particles were treated with the non-fluorescent probe dichlorofluorescin (DCFH) that fluoresces when oxidized by the presence of ROS. The measured fluorescent intensities were converted into equivalent hydrogen peroxide concentrations, which were used as indicators of ROS reactivity, by calibrations using H 2 O 2 standards. Diurnal profiles of the ROS concentrations were obtained. Correlations of the particulate ROS concentrations with the intensity of photochemical reaction, estimated secondary organic carbon (SOC) and gas phase OH and HO 2 radical concentrations were explored. The intensity of photochemical reactions and gas phase radical concentrations were found to be moderate factors affecting particulate ROS concentrations. The concentrations of ROS were found to be higher in the submicron size particles of the ambient aerosol.

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“…Both cellular (Xia et al, 2006;Kubátová et al, 2006;Bonvallot et al, 2001;Hiura et al, 1999;Antonini et al, 1998) and acellular methods (Zomer et al, 2011;Mudway et al, 2011;Jung et al, 2006;Cho et al, 2005;Venkatachari et al, 2005;Frampton et al, 1999) have been developed to measure the oxidative potential of PM. Both types of methods have advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

A semi-automated system for quantifying the oxidative potential of ambient particles in aqueous extracts using the dithiothreitol (DTT) assay: results from the Southeastern Center for Air Pollution and Epidemiology (SCAPE)

et al. 2015

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Abstract. A variety of methods are used to measure the capability of particulate matter (PM) to catalytically generate reactive oxygen species (ROS) in vivo, also defined as the aerosol oxidative potential. A widely used measure of aerosol oxidative potential is the dithiothreitol (DTT) assay, which monitors the depletion of DTT (a surrogate for cellular antioxidants) as catalyzed by the redox-active species in PM. However, a major constraint in the routine use of the DTT assay for integrating it with large-scale health studies is its labor-intensive and time-consuming protocol. To specifically address this concern, we have developed a semi-automated system for quantifying the oxidative potential of aerosol liquid extracts using the DTT assay. The system, capable of unattended analysis at one sample per hour, has a high analytical precision (coefficient of variation of 15 % for positive control, 4 % for ambient samples) and reasonably low limit of detection (0.31 nmol min −1 ). Comparison of the automated approach with the manual method conducted on ambient samples yielded good agreement (slope = 1.08 ± 0.12, r 2 = 0.92, N = 9). The system was utilized for the Southeastern Center for Air Pollution & Epidemiology (SCAPE) to generate an extensive data set on DTT activity of ambient particles collected from contrasting environments (urban, roadside, and rural) in the southeastern US. We find that water-soluble PM 2.5 DTT activity on a per-air-volume basis was spatially uniform and often well correlated with PM 2.5 mass (r = 0.49 to 0.88), suggesting regional sources contributing to the PM oxidative potential in the southeastern US. The correlation may also suggest a mechanistic explanation (oxidative stress) for observed PM 2.5 mass-health associations. The heterogeneity in the intrinsic DTT activity (per-PM-mass basis) across seasons indicates variability in the DTT activity associated with aerosols from sources that vary with season. Although developed for the DTT assay, the instrument can also be used to determine oxidative potential with other acellular assays.

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Freshly generated stainless steel welding fume induces greater lung inflammation in rats as compared to aged fume

Cited by 71 publications

References 27 publications

Exposure to nanoparticles is related to pleural effusion, pulmonary fibrosis and granuloma

Exposure to nanoparticles is related to pleural effusion, pulmonary fibrosis and granuloma

Characterization of Wintertime Reactive Oxygen Species Concentrations in Flushing, New York

A semi-automated system for quantifying the oxidative potential of ambient particles in aqueous extracts using the dithiothreitol (DTT) assay: results from the Southeastern Center for Air Pollution and Epidemiology (SCAPE)

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