Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers

Teepe, R.G.C.; Koebrugge, Eline J.; Ponec, Maria; Vermeer, B.J.

doi:10.1111/j.1365-2133.1990.tb08243.x

Cited by 106 publications

(58 citation statements)

References 30 publications

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“…12 Second, it took 2 weeks to generate the complete skin equivalent in the present study, which is too long for the management of acute wounds. Cryopreserved allogenic cultured skin grafts have offered the possibility of initial treatment; 24,25 even though they are ultimately rejected, the allogenic graft can accelerate the wound-healing process, probably by inducing some important growth factors. 26 The lack of pilosebaceous units in the cultured skin equivalent is another problematic issue.…”

Section: Discussionmentioning

confidence: 99%

Epidermal Structure Created by Canine Hair Follicle Keratinocytes Enriched with Bulge Cells in a Three‐Dimensional Skin Equivalent Model in Vitro: Implications for Regenerative Therapy of Canine Epidermis

Kobayashi¹,

Enomoto²,

Wang³

et al. 2013

Advances in Veterinary Dermatology

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Section: Discussionmentioning

confidence: 99%

Epidermal Structure Created by Canine Hair Follicle Keratinocytes Enriched with Bulge Cells in a Three‐Dimensional Skin Equivalent Model in Vitro: Implications for Regenerative Therapy of Canine Epidermis

Kobayashi¹,

Enomoto²,

Wang³

et al. 2013

Advances in Veterinary Dermatology

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“…In the literature, several authors have described the cultured epidermal allografts for freezing, expanding its availability and making your employment as a viable clinical alternative 3,5,6,13 . Thus, the cryopreservation of cultured epithelium has been a constant search topic in many centers worldwide 3,5,[13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the possibility to transfer skin has greatly improved the restoration of tissue defects following traumas, clinical disease or major traumas [1][2][3][4][5][6][7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

et al. 2013

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PURPOSE:To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. METHODS:Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70 o C. After freezing for a period of time ranging from one to 12 months, cultured epithelial samples were assessed for their viability by vital staining (Trypan blue) and metabolic analysis based on glucose consumption and lactate production. Samples of not frozen cultured epithelium (Group B) were also tested for viability and the results obtained were used as comparison parameter for the variation of viability. RESULTS:Statistical analysis between the group A and B indicate that the mean age of the donors (p=0.51) and the culture time (p=1.18) showed no statistical difference. In 30 days we obtained 37% of the original viability of cultured epithelium, 25% at six months and one year, less than 15%. This trend was confirmed statistically with a reduction of approximately 1.8% of the original viability epithelium cultured every 30 days of storage. In the analysis by lactate production, similar results were observed. In the analysis by the glucose consumption results were not significant. The viability indices show statistically significant difference between the group A and B (p<0.0001). CONCLUSIONS:Although cryopreserved cultured epithelium showed significant reduction of viability, all samples remained viable. It was also found that the viability of cryopreserved cultured epithelial decreased as a function of storage time.

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“…51 In 1990, Teepe et al estimated a cell viability of 62.8% by the dye exclusion method (trypan blue) in cryopreserved cultured epithelial grafts stored for 6 weeks at −70°C. 52 More recently, Udoh et al compared cell viability measures of CPAs stored at −135°C and −80°C: median cell survival rates were 89.3%, 61.7% and 61.6% after 1, 6 and 12 months of storage at −135°C, respectively, whereas at −80°C, survival rates were much lower (35.2% viability after 6 months). 48 Colony-forming efficiency of grafts cryopreserved for 1, 6 and 12 months was estimated at 66.1%, 58.5% and 55.1%, respectively, of noncryopreserved control grafts.…”

Section: Long-term Storage Studiesmentioning

confidence: 99%

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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Donor skin and dermal grafts are used in several types of loss of substance for different clinical purposes. As biological physiological medication, donor skin grafts can promote re-epithelization, shorten healing time, alleviate pain and protect dermal and subcutaneous structures such as cartilage, tendons, bones and nerves. Though a great variety of dermal matrices and skin equivalents, both synthetic and semisynthetic, are now available for wound treatment, viable human skin allografts remain an important therapeutic choice for extensive deep burns and hard-to-heal wounds. In such cases, viable skin allografts have significantly better clinical outcomes than unviable human-derived allografts or synthetic medications. The demand for human-derived skin bioproducts continues to be a reason for the existence of skin banks. Skin bank organization is complex and requires continuous updating. Careful donor selection, thorough microbiological and serological donor screening for transmissible diseases and rigorous quality control during tissue preparation are necessary to minimize the risk of transmission of pathogenic agents. Skin banks must also observe standardized reproducible procedures to ensure tissue traceability and biological safety in all phases of processing and to avoid new biological contamination. Constant training and periodic checks are needed to keep skin bank operators attentive and responsible. Finally, skin banks should guarantee collection and storage of highly viable skin. Here, we discuss available tissue storage methods and the different types of skin bioproducts.

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Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers

Cited by 106 publications

References 30 publications

Epidermal Structure Created by Canine Hair Follicle Keratinocytes Enriched with Bulge Cells in a Three‐Dimensional Skin Equivalent Model in Vitro: Implications for Regenerative Therapy of Canine Epidermis

Epidermal Structure Created by Canine Hair Follicle Keratinocytes Enriched with Bulge Cells in a Three‐Dimensional Skin Equivalent Model in Vitro: Implications for Regenerative Therapy of Canine Epidermis

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

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