Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport

Antonenas, Vicki; Garvin, Frances; Webb, M.; Sartor, Mary; Bradstock, Ken; Gottlieb, David

doi:10.1080/14653240600620994

Cited by 46 publications

(43 citation statements)

References 14 publications

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“…Release of lactic acid has been described to correlate with lower viability in stored cells, especially for high cell concentrations. However, cell density was considerably lower in the CD133 + cell suspension we employed than in BM or comparable cell products (2,17). The apoptosis rate of freshly isolated CD133 + stem cells may seem considerable.…”

Section: Discussionmentioning

confidence: 92%

Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells

et al. 2015

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Stem cell transplantation is a viable strategy for regenerative medicine. However, it is inevitable to have cells undergo storage for several hours or days due to processing and transportation. Therefore, it is crucial to have rigidly controlled conditions ensuring the therapeutic benefit of isolated stem cells. In the present study, we investigated the impact of short-term storage on human CD133(+) cells. CD133(+) cells were isolated from human bone marrow and kept at standardized nonfreezing storage conditions for up to 72 h. Cell viability (apoptosis/necrosis) and expression of CD133 and CXCR4 were analyzed by flow cytometry. Metabolic activity was determined using an MTT assay; colony-forming ability, as well as endothelial-like differentiation, was further evaluated. A qRT-PCR array was employed to investigate the expression of stemness genes. CD133 and CXCR4 expressions were preserved at all time points. After 30 h, cell number and metabolic activity decreased, although no significant changes were detected in cell viability and proliferation as well as endothelial-like differentiation. Cell viability and proliferation decreased significantly only after 72 h of storage. Our results indicate that storage of isolated human CD133(+) bone marrow stem cells in liquid allows for high viability and functionality. However, storage time should be limited in order to avoid cell loss.

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Section: Discussionmentioning

confidence: 92%

Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells

et al. 2015

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“…The numbers of studies analyzing the impact of shipment on fluctuating temperatures on flow cytometry analysis is limited and were mainly done on controlled temperature to mimic shipment conditions (Antonenas et al, 2006;Ekong et al, 1993). Studies conducted by Ahmann et al (2008) showed that tissue sample shipment on dry ice within USA did not have a drastic impact on plasma cell isolation, viability and RNA integrity.…”

Section: Discussionmentioning

confidence: 99%

Optimal cellular preservation for high dimensional flow cytometric analysis of multicentre trials

Lee

Teo

et al. 2012

Journal of Immunological Methods

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“…When cells are refrigerated (2°–8°C), PBSC CD34 + viability is slowly reduced at 48 and 72 hours. The same conditions have a limited effect on CD34 viability of BM cells (25), while other factors that affect cell viability include shipping, storage conditions, and cell concentration (26). …”

Section: Discussionmentioning

confidence: 99%

Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34 + cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers

et al. 2014

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Background Evaluation of the BD™ Stem Cell Enumeration (SCE) Kit was conducted at four clinical sites with flow cytometry CD34+ enumeration, to assess agreement between two investigational methods, the BD FACSCanto™ II and BD FACSCalibur™ systems, and the predicate method (Beckman Coulter Stem-Kit™ reagents). Methods Leftover and delinked specimens (n = 1,032) from clinical flow cytometry testing were analyzed on the BD FACSCanto II (n = 918) and BD FACSCalibur (n = 905) in normal and mobilized blood, frozen and thawed bone marrow, and leucopheresis and cord blood anticoagulated with CPD, ACD-A, heparin, and EDTA alone or in combination. Fresh leucopheresis analysis addressed site equivalency for sample preparation, testing, and analysis. Results The mean relative bias showed agreement within predefined parameters for the BD FACSCanto II (−2.81 to 4.31 ±7.1) and BD FACSCalibur (−2.69 to 5.2 ±7.9). Results are reported as absolute and relative differences compared to the predicate for viable CD34+, percentage of CD34+ in CD45+, and viable CD45+ populations (or gates). Bias analyses of the distribution of the predicate low, mid, and high bin values were done using BD FACSCanto II optimal gating and BD FACSCalibur manual gating for viable CD34+, percentage of CD34+ in CD45+, and viable CD45+. Bias results from both investigational methods show agreement. Deming regression analyses showed a linear relationship with R2 >0.92 for both investigational methods. Discussion In conclusion, the results from both investigational methods demonstrated agreement and equivalence with the predicate method for enumeration of absolute viable CD34+, percentage of viable CD34+ in CD45+, and absolute viable CD45+ populations.

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Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport

Cited by 46 publications

References 14 publications

Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells

Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells

Optimal cellular preservation for high dimensional flow cytometric analysis of multicentre trials

Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34 + cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers

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Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport

Cited by 46 publications

References 14 publications

Impact of Short-Term Liquid Storage on Human CD133+ Stem Cells

Impact of Short-Term Liquid Storage on Human CD133+ Stem Cells

Optimal cellular preservation for high dimensional flow cytometric analysis of multicentre trials

Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34 + cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers

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Product

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Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells

Impact of Short-Term Liquid Storage on Human CD133⁺ Stem Cells