Frequent Occurrence of Extended-Spectrum Beta-Lactamase- and Transferable AmpC Beta-Lactamase-Producing Escherichia coli on Domestic Chicken Meat in Sweden

Börjesson, Stefan; Egervärn, Maria; Lindblad, M.; Englund, Stina

doi:10.1128/aem.03893-12

Cited by 50 publications

(39 citation statements)

References 26 publications

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“…The inability to persist on animal level might be due to an unsuccessful combination of CMY-2-E. coli on plasmid IncA/C. In European broiler meat, CMY-2-E. coli is often found in combination with plasmids IncI1 or IncK (Borjesson et al, 2013;Egervarn et al, 2014). The low occurrence and the observed decrease in this study may suggest that plasmid IncA/C is less able to conjugate and spread in bacterial populations as was previously described for Salmonella (Poole and Crippen, 2009).…”

Section: Resultscontrasting

confidence: 45%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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A B S T R A C TExtended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pAmpC) producing bacteria are resistant to Extended Spectrum Cephalosporins (ESC), and are present in all levels of the broiler production chain. We determined the prevalence, concentration, and persistence of ESBL/pAmpC-Escherichia coli in a broiler parent flock during the rearing and laying period. One-day old chickens were housed in four separate pens. Until week 33 no antibiotics or coccidiostatics were used. During rearing 57 chickens in each pen (n = 228), and in the laying period two groups of 33 chickens were individually sampled (n = 66). Environmental samples were taken from week 16 onwards. ESBL/pAmpC-E. coli presence was determined by selective culturing. In the samples of week 16-19 the concentration of ESBL/pAmpC-E. coli was determined. All ESC-resistant isolates found were positive for pAmpC gene bla CMY-2 located on IncA/C plasmids, in several E. coli MLST types. CMY-2-E. coli prevalence decreased from 91% (95%CI 86-94%) at day 7 (week 1) to 0% (95%CI 0-5%) in week 21. However, CMY-2-E. coli remained present in the environmental samples during the whole study. CMY-2-E. coli concentration varied between detection limit (< 10^3) and 2·10^4 cfu/g faeces. The sharp reduction of CMY-2-E. coli in this broiler parent flock in absence of antibiotics suggests a selective disadvantage of bla CMY-2 on IncA/C plasmids on animal level. The underlying mechanism should be studied further as this may provide new insights on how to reduce ESBL/pAmpC prevalence and transmission in the broiler production chain.

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Section: Resultscontrasting

confidence: 45%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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“…Our results showed that 96.5 % of the ESBL-E. coli isolates encoded genes of the CTX-M group with or without TEM-encoding genes, suggesting the emergence of the CTX-M gene group as the main contributor to the antibiotic resistance of ESBL-E. coli in food, foodproducing animals, healthy people, and patients not only in developed countries but also in underdeveloped countries (Sasaki et al 2010;Overdevest et al 2011;Börjesson et al 2013;Chen et al 2014;Nakayama et al 2015).…”

Section: Discussionmentioning

confidence: 96%

Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

Kawahara

Khong

et al. 2015

FoodContamination

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Background: Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potential sources of contamination. Results: A total of 253 food samples from poultry, pork, shrimp, and fish were collected from local markets in a rural area of Vietnam from 2013 to 2014. ESBL-producing Escherichia coli were isolated from these samples, and their ESBL genotypes, phylogenetic groups, and antibiotic resistance profiles were assessed. Overall, a high percentage (68.4 %) of the food samples was contaminated with ESBL-producing E. coli, and samples from livestock and fishery products showed similar levels of contamination. The ESBL-producing E. coli isolated from the food samples harbored genes of the CTX-M-1, CTX-M-9, and TEM groups. Analysis of the antibiotic resistance profiles of the ESBL-producing E. coli isolates from the food samples showed a high degree of multidrug resistance. The prevalence of extensively multidrug-resistant ESBL-producing E. coli that were resistant to at least five antibiotic drug classes in poultry, pork, shrimp, and fish samples, was 92.1, 69, 56.5, and 62.5 %, respectively. Conclusions: The results of this study confirmed the widespread dissemination of ESBL-producing E. coli in both livestock and fishery products from a rural area in Vietnam. The high prevalence of extensively multidrug-resistant ESBL-producing E. coli in food products highlights the importance of continuous monitoring of food products for the presence of these bacteria, particularly in underdeveloped countries.

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“…These organisms could serve as a reservoir for the genes encoding antimicrobial resistance, which is transferred to the pathogenic bacteria or transmitted to the human by direct exposure to animals or via the food chain. [15][16][17][18][19] It has been shown that the resistant bacteria with animal origin can either colonize or cause infection in humans.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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Background: Food-producing animals are under suspicion for the reservoir and colonization of ESBL (extended-spectrum beta-lactamase)-producing bacteria especially Enterobacteriaceae and therefore infection of the humans with them. The increasing reports on the ESBLs presence in the pathogenic and commensal Escherichia coli isolates have been a concern worldwide. These strains can be attributed to one of the main phylogenetic groups and subgroups. Several studies have shown the relationship between the phylogeny and antimicrobial resistance of E. coli strains. Objectives: The aim of this study was to analyze the phylogenetic group of ESBL-producing E. coli and detect its phenotype using the multiplex polymerase chain reaction (PCR) and combined disk method. Materials and Methods: Two hundred five E. coli fecal isolates were obtained from 103 calves (90 healthy and 13 diarrheic) and 102 dairy cows (healthy) from 8 farms in Khuzestan province, Iran. The triplex PCR method was used to allocate the E. coli isolates based on the presence or absence of 3 genes (chuA, yjaA, and tspE4.C2) to yield 4 definite phylogenetic groups and 7 subgroups. Phenotypic ESBL-producing E. coli was determined using the double disk diffusion method according to the manufacturer's instructions and Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: A total of 65.04% and 22.3% of isolates from calves and 70.5% and 20.5% of isolates from dairy cows belonged to phylogroups B1 and A, respectively. In addition, no isolate from the diarrhoeic calves was found to belong to group B2 and subgroups D2 and A0. A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the samples of dairy cows which belonged to the phylogenetic group A and phylogenetic subgroup A1. There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between the E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Conclusion: There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Based on these results, there is a low prevalence of ESBL-producing E. coli in the dairy farms of Khuzestan province. However, further large scale investigations are necessary to control the antibiotic resistance in the human and animal foodstuff.

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Frequent Occurrence of Extended-Spectrum Beta-Lactamase- and Transferable AmpC Beta-Lactamase-Producing Escherichia coli on Domestic Chicken Meat in Sweden

Cited by 50 publications

References 26 publications

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

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