Frequent genital herpes simplex virus 2 shedding in immunocompetent women. Effect of acyclovir treatment.

Wald, Anna; Corey, Lawrence; Cone, Richard W.; Hobson, Ann C.; Davis, Gray; Zeh, Judy

doi:10.1172/jci119237

Cited by 353 publications

(257 citation statements)

References 34 publications

Supporting

Mentioning

242

Contrasting

Unclassified

Order By: Relevance

“…Subjects were instructed on the daily sampling of genital and oral mucosal sites as described in detail previously (27,28). Dacron swabs placed in viral transport medium were delivered to the laboratory three times per week.…”

Section: Daily Home Sampling Of Mucosal Sites and Detection Of Hsv Bymentioning

confidence: 99%

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Posavad

Wald

Hosken³

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

During the course of investigating T cell responses to HSV among volunteers entering trials of investigational genital herpes vaccines, 6 of the 24 immunocompetent subjects with no prior history of oral/labial or genital herpes possessed HSV-specific T cell immunity but, by multiple determinants of even the most sensitive serological assays, remained seronegative to HSV-1 and -2. Of these six immune seronegative (IS; HSV-seronegative with HSV-specific T cell responses) subjects, two had transient HSV-specific T cell responses, while four had CD4+ and CD8+ T cell responses directed at HSV that persisted for up to 4 years. CD4+ T cell clones were isolated that recognized and had high binding affinities to epitopes in HSV-2 tegument proteins. All six IS subjects had potential sexual exposure to an HSV-2-infected sexual partner. Oral and genital mucosal secretions were sampled and tested for the presence of infectious HSV and HSV DNA. No evidence of HSV was detected in >1500 samples obtained from these IS subjects. The identification of persistent T cell responses to HSV in seronegative subjects is a novel finding in the herpesvirus field and suggests either undetected infection or acquired immunity in the absence of infection. Understanding the basis of these acquired immune responses may be critical in developing effective vaccines for genital herpes.

show abstract

Section: Daily Home Sampling Of Mucosal Sites and Detection Of Hsv Bymentioning

confidence: 99%

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Posavad

Wald

Hosken³

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…After primary infection at mucosal sites, HSV-2 remains latent in neuronal cells with intermittent HSV reactivations, resulting in the production of infectious virus with or without the onset of discernible disease. In immunocompetent persons infected with HSV-2, viral reactivation is high and occurs on an average of 20% of days (2). The high rate of HSV-2 shedding at mucosal sites suggests that the immune system is frequently or even chronically exposed to HSV Ag and likely results in the intermittent restimulation of HSV-specific T cells.…”

mentioning

confidence: 99%

Long Term Persistence of Herpes Simplex Virus-Specific CD8+ CTL in Persons with Frequently Recurring Genital Herpes

Posavad

Huang²,

Barcy³

et al. 2000

The Journal of Immunology

View full text Add to dashboard Cite

Herpes simplex virus (HSV) establishes a lifelong infection in humans. Reactivation of latent virus occurs intermittently so that the immune system is frequently exposed to viral Ag, providing an opportunity to evaluate memory T cells to a persistent human pathogen. We studied the persistence of genital herpes lesion-derived HSV-specific CD8+ CTL from three immunocompetent individuals with frequently recurring genital HSV-2 infection. All CTL clones were HSV-2 type specific and only one to three unique clonotypes were identified from any single biopsy specimen. The TCRBV genes utilized by these clonotypes were sequenced, and clonotype-specific probes were used to longitudinally track these clonotypes in PBMC and genital lesions. CTL clonotypes were consistently detected in PBMC and lesions for at least 2 and up to 7 years, and identical clonotypes infiltrated herpes lesions spaced as long as 7.5 years apart. Moreover, these clones were functionally lytic in vivo over these time periods. Additionally, CTL clones killed target cells infected with autologous viral isolates obtained 6.5 years after CTL clones were established, suggesting that selective pressure by these CTL did not result in the mutation of CTL epitopes. Thus, HSV recurs in the face of persistent CD8+ CTL with no evidence of clonal exhaustion or mutation of CTL epitopes as mechanisms of viral persistence.

show abstract

“…Су-прессивная терапия ациклическими нуклеозидами снижает количество рецидивов и эпизодов вирусо-выделения (80-95%), в том числе и в дискордант-ных парах [16,17].…”

Section: ключевые слова: генитальный герпес рецидивирующее течение unclassified

Systemic therapy of patients with recurrent genital herpes

Кузнецова¹,

Kuznetsova²,

Кунгуров³

et al. 2015

Klin. dermatol. venerol.

View full text Add to dashboard Cite

Социальная значимость герпесвирусной инфек-ции (ГВИ) обусловлена прежде всего достаточно широким ее распространением. Каждый пятый взрослый серопозитивен по отношению к вирусу простого герпеса (ВПГ) 2-го типа, являясь чаще все-го асимптомным носителем. В США только 20% па-циентов, обратившихся с симптомами генитальной ГВИ, установлен правильный диагноз [1]. Более 50 млн жителей США инфицированы ВПГ [2]. Еже-годно регистрируют до 1,5 млн новых случаев [3]. В Швеции 70% жителей инфицированы ВПГ 1-го типа (ВПГ-1) и 20% -ВПГ 2-го типа (ВПГ-2) [4]. Учитывая, что данную инфекцию характеризует по-жизненная персистенция возбудителя, первичная заболеваемость выше среди молодых, а общая уве-личивается с возрастом [5,6]. Женщины чаще, чем

show abstract

Frequent genital herpes simplex virus 2 shedding in immunocompetent women. Effect of acyclovir treatment.

Cited by 353 publications

References 34 publications

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Long Term Persistence of Herpes Simplex Virus-Specific CD8+ CTL in Persons with Frequently Recurring Genital Herpes

Systemic therapy of patients with recurrent genital herpes

Contact Info

Product

Resources

About