Frequent contamination of edible freshwater fish with colistin-resistant Escherichia coli harbouring the plasmid-mediated mcr-1 gene

Hoa, Trần Thị Tuyết; Huyền, Hồng Mộng; Nakayama, Tadasi; Minh, Doan Tran Nguyen; Hoang, Oanh Nguyen; Thi, Hien Le; Thanh, Phong Ngo; Hoai, Phuong Hoang; Yamaguchi, Takahiro; Jinnai, Michio; Nguyen, Phuc Do; Van, Chinh Dang; Kumeda, Yuko; Hase, Atsushi

doi:10.1016/j.marpolbul.2022.114108

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Previously, CTX-resistant E. coli has been identified in 61% of pond-reared striped catfish in the Mekong Delta [ 52 ]. Another study found that a 14.6% prevalence of ESBL-resistant E. coli in freshwater fish sold in wet markets in the Mekong Delta [ 53 ]. Further studies should determine possible transfer of the IncA/C and associated resistance genes between E. ictaluri and E. coli .…”

Section: Discussionmentioning

confidence: 99%

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Erickson,

Dung,

Khoi

et al. 2024

Microorganisms

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Edwardsiella ictaluri is responsible for causing bacillary necrosis (BNP) in striped catfish (Pangasianodon hypophthalmus) in Vietnam. This study offers a comprehensive genomic characterization of E. ictaluri to enhance understanding of the molecular epidemiology, virulence, and antimicrobial resistance. E. ictaluri isolates were collected from diseased striped catfish in the Mekong Delta. The species was confirmed through PCR. Antimicrobial susceptibility testing was conducted using minimum inhibitory concentrations for commonly used antimicrobials. Thirty representative isolates were selected for whole genome sequencing to delineate their genomic profiles and phylogeny. All strains belonged to ST-26 and exhibited genetic relatedness, differing by a maximum of 90 single nucleotide polymorphisms. Most isolates carried multiple antimicrobial resistance genes, with the tet(A) gene present in 63% and floR in 77% of the genomes. The ESBL gene, blaCTX-M-15, was identified in 30% of the genomes. Three plasmid replicon types were identified: IncA, p0111, and IncQ1. The genomes clustered into two clades based on their virulence gene profile, one group with the T3SS genes and one without. The genetic similarity among Vietnamese isolates suggests that disease spread occurs within the Mekong region, underscoring the importance of source tracking, reservoir identification, and implementation of necessary biosecurity measures to mitigate spread of BNP.

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Section: Discussionmentioning

confidence: 99%

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Erickson,

Dung,

Khoi

et al. 2024

Microorganisms

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“…ESBL-producing Escherichia coli is frequently detected in humans and food (Hoa et al, 2020;Nakayama et al, 2015). We recently revealed the contamination of food products with plasmid-mediated colistinresistant E. coli (Hoa, 2022). In particular, colistin-resistant E. coli contamination in chicken meat was detected at the same high frequency as ESBL-producing E. coli (Nakayama et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

Nakayama,

Li,

Ngo

et al. 2024

Journal of Food Safety

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Food contamination with plasmid‐mediated antibiotic‐resistant Escherichia coli has not been thoroughly investigated. This study aimed to clarify the prevalence of colistin‐resistant AmpC/extended‐spectrum β‐lactamase (ESBL)‐producing E. coli harbouring mcr (COL‐ESBL‐EC) and to determine antibiotic resistance by comparison with AmpC/ESBL‐producing E. coli (ESBL‐EC) and colistin‐resistant E. coli harbouring mcr (COL‐EC). Sixty chicken meats were tested for COL‐ESBL‐EC contamination. The result showed that 64 COL‐ESBL‐EC were isolated from 66.7% of the samples, and compared with ESBL‐EC and COL‐EC were isolated before. The genotypes of the COL‐ESBL‐EC showed blaCTX‐M‐55/TEM, blaCTX‐M‐55, blaCMY‐2, and blaCTX‐M‐65 were predominant. The COL‐ESBL‐EC results showed a similar trend for blaCTX‐M identification to that of ESBL‐EC. The mcr was investigated, and mcr‐1 was detected in COL‐ESBL‐EC and COL‐EC. Antibiotic susceptibility testing revealed that many strains of COL‐ESBL‐EC and ESBL‐EC were resistant to quinolones, and fosfomycin (FOS). These results suggest that COL‐ESBL‐EC has simultaneously acquired AmpC/ESBL‐related, quinolone, and FOS resistance genes in COL‐EC.

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Frequent contamination of edible freshwater fish with colistin-resistant Escherichia coli harbouring the plasmid-mediated mcr-1 gene

Cited by 2 publications

References 28 publications

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

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Frequent contamination of edible freshwater fish with colistin-resistant Escherichia coli harbouring the plasmid-mediated mcr-1 gene

Cited by 2 publications

References 28 publications

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Genomic Insights into Edwardsiella ictaluri: Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Acquisition of cephalosporin resistance genes blaCTX‐M‐55, blaCTX‐M‐65, and blaCMY‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene

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Acquisition of cephalosporin resistance genes bla_CTX‐M_‐55, bla_CTX‐M_‐65, and bla_CMY_‐2 leads to quinolone resistance in colistin‐resistant Escherichia coli harboring mcr‐1 gene