Frequency of transferable multiple antibiotic resistance amongst coliform bacteria isolated from a treated sewage effluent in Antofagasta, Chile

Silva, Juan; Castillo, G. F. Torres del; Callejas, Lorena; Lopez, Hector Sanchez; Olmos, Janet

doi:10.2225/vol9-issue5-fulltext-7

Cited by 59 publications

(38 citation statements)

References 14 publications

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“…The disposal of treated sewage into rivers, lakes or elsewhere may or may not influence environmental bacterial populations (Sloan et al, 2014). Some studies have found that wastewater treatment can raise or lower the proportions of antibiotic resistant bacteria which carry antibiotic resistance plasmids (Silva et al, 2006). The observation of increased resistance frequency to ampicillin, tetracycline, streptomycin and chloramphenicol after wastewater treatment has previously been reported by Reinthaler et al (2013).…”

Section: Discussionmentioning

confidence: 81%

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Akhter¹,

Imran²,

Akhter³

2014

Afr. J. Microbiol. Res.

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The antibiotic resistance profiles of Escherichia coli isolated from three different sampling sites of the Gomti River at Lucknow city was evaluated. Water samples were collected and then analyzed for the presence of E. coli, using standard methods. Antibiotic susceptibility testing was performed by the disc diffusion method. Of the 77 E. coli isolates tested, marked antibiotic resistances (over 70%) were observed for amoxicillin, nitrofurozone, chloramphenicol, polymixin B, methicilin, ampicillin, nalidixic acid, cefpodoxime, erythromycin, penicillin, rifampicin and ofloxacin depending upon the sampling sites. All E. coli isolates also showed multiple resistance patterns in different combination of antibiotics. The MAR index ranges were found very high indicating the high risk of environmental contamination. The findings indicated that pollution of aquatic environments from different sources of the city may have a potential impact on the dissemination and survival of E. coli, as well as other pathogenic bacteria in the Gomti River water for public and animal health. This may result to a negative effect on antibiotic therapy for infectious diseases.

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Section: Discussionmentioning

confidence: 81%

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Akhter¹,

Imran²,

Akhter³

2014

Afr. J. Microbiol. Res.

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“…The same tendency was observed in case of erythromycin resistant bacteria, with the highest number in winter. The increase of the number of resistant bacteria could be caused by the higher usage of this antibiotic to treat bacteria diseases (Figure 1b), because as it was previously stated, the exposition to the drug can cause the increase of bacterial resistance (Silva et al 2006, Soufi et al 2010. Because erythromycin is known to possess wider bactericidal spectrum and it is used more often than sulfamethoxazole/trimethoprim, it is possible that its usage increased during winter.…”

Section: Discussionmentioning

confidence: 89%

Detection of antibiotic resistance genes in wastewater treatment plant – molecular and classical approach

Ziembińska-Buczyńska

Felis

Folkert

et al. 2015

Archives of Environmental Protection

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Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confi rmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L , respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confi rmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary.

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“…Little is currently known about the dynamics of antibiotic-resistant bacteria and genes encoding antibiotic resistance in wastewater treatment plants (WWTPs). It has been speculated that the wastewater treatment process may increase the proportion of resistant bacteria in outlet (Silva et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant

et al. 2009

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The spread of methicillin-resistant Staphylococcus aureus (MRSA), in which the mecA gene mediates resistance, threatens the treatment of staphylococcal diseases. The aims were to determine the effect of wastewater treatment processes on mecA gene concentrations, and the prevalence of S. aureus and MRSA over time. To achieve this a municipal wastewater treatment plant was investigated for the mecA gene, S. aureus and MRSA, using real-time PCR assays. Water samples were collected monthly for one year, at eight sites in the plant, reflecting different aspects of the treatment process. The mecA gene and S. aureus could be detected throughout the year at all sampling sites. MRSA could also be detected, but mainly in the early treatment steps. The presence of MRSA was verified through cultivation from inlet water. The concentration of the mecA gene varied between months and sampling sites, but no obvious seasonal variation could be determined. The wastewater treatment process reduced the mecA gene concentration in most months. Taken together our results show that the mecA gene, S. aureus and MRSA occur over the year at all sites investigated.

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Frequency of transferable multiple antibiotic resistance amongst coliform bacteria isolated from a treated sewage effluent in Antofagasta, Chile

Cited by 59 publications

References 14 publications

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Detection of antibiotic resistance genes in wastewater treatment plant – molecular and classical approach

A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant

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