Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure

Kishi, Kanako

doi:10.7860/jcdr/2015/10332.6811

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…Nosrati et al (2014) reported the improvement of human spermatozoa selected by a microfluidic device composed of 500 parallel microchannels in terms of reducing more than 80% DNA fragmentation. Similar results were reported by Kishi et al (2015), showing a significant reduction of DNA fragmentation in spermatozoa selected by a commercially available device based on microfluidics and flow dynamics (Sperm Sorter Qualis ® , Menicon, Kasugai, Japan) when compared to SU or unselected spermatozoa. Using the same device, Shirota et al (2016) also reported the selection of spermatozoa with lower DNA fragmentation compared to spermatozoa selected by DGC followed by SU in healthy donors.…”

Section: Sperm Selection Based On Sperm Motility (Microfluidics)supporting

confidence: 85%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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Almost 50% of the infertility cases are due to male factors. Assisted reproductive technologies (ARTs) allow to overcome the incapacity of these patients' spermatozoa to fertilize the oocyte and produce a viable and healthy offspring, but the efficiency of the different techniques has still the potential to improve. According to the latest reports of the European Society of Human Reproduction and Embryology (ESHRE) and the Centers for Disease Control and Prevention of the United States (CDC), the percentages of deliveries per ART cycle in 2014 and 2016 were 21 and 22%, respectively. Among the reasons for this relatively low efficiency, the quality of the spermatozoa has been pointed out as critical, and the presence of high percentages of DNA-damaged spermatozoa in patients' ejaculates is possibly one of the main factors reducing the ARTs outcomes. Thus, one of the main challenges in reproductive medicine is to ensure the highest quality of the spermatozoa used in ARTs, and specifically, in terms of genetic integrity. The latest techniques for the preparation and selection of human spermatozoa are herein discussed focusing on those proven to improve one or several of the following parameters: sperm genetic integrity, fertilization capacity, embryo production, and in vitro survival, as well as pregnancy and delivery rates following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). In addition, we discuss the potential of techniques developed in non-human mammals that could be further transferred to the clinic.

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Section: Sperm Selection Based On Sperm Motility (Microfluidics)supporting

confidence: 85%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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“…No clinical ART data was reported. Other studies revealed that the use of microfluidic devices resulted in the selection of sperm with reduced DNA damage in comparison to the swim-up technique ( Kishi et al ., 2015 ; Shirota et al ., 2016 ). The group of men studied, however, was small, heterogeneous, and included both fertile and infertile individuals, thus preventing solid conclusions.…”

Section: Resultsmentioning

confidence: 99%

Sperm selection in IVF: the long and winding road from bench to bedside

Pedrosa¹,

Furtado

Ferreira³

et al. 2020

JBRA Assisted Reproduction

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Spermatozoa wage battle to conquer fertilization but the traits needed to succeed remain elusive. The natural advantageous qualities that enable only a few selected sperm cells to reach the site of fertilization remain unknown. Although in vitro fertilization (IVF) facilitates the job of spermatozoa, a universally acceptable means of sperm selection is yet to be developed. No objective or reliable sperm quality indicators have been established and sperm selection is, to a great extent, based on subjective qualitative evaluation. The best method for sperm selection in IVF presents several challenges: intrinsic sperm qualities cannot be evaluated and the ideal endpoint for these studies is debatable. An ideal method for sperm selection in ART should be noninvasive and cost-effective, and allow the identification of high-quality spermatozoa and yield better outcomes in terms of pregnancy and live birth rates. This narrative review included 85 papers and focused on the new available methods and technologies that might shed some light on sperm selection in IVF. It discusses the available data on microfluidic devices, omics profiling, micronuclei studies, sperm plasma membrane markers, and other techniques, such as Magnetic Activated Cell Sorting (MACS), Raman micro-spectroscopy, and artificial intelligence systems. The new techniques herein reviewed offer fresh approaches to an old problem, for which a definite solution has yet to cross the bridge from bench to IVF clinics around the world, since clinical usefulness and application remain unproven.

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“…Newer technologies, such as microfluidics, electrophoresis, motile sperm organelle morphology examination (MSOME), and birefringence have been advocated for processing human semen [54]. One example is that the microfluidic device was used to select spermatozoa with better chromatin fragmentation, as evaluated by the DNA dispersion test, from oligospermic human samples [55]. However, such methods are too expensive and impractical to be considered for processing animal semen.…”

Section: Additional Selection Techniquesmentioning

confidence: 99%

Colloid Centrifugation of Semen: Applications in Assisted Reproduction

Morrell¹,

Rodriguez‐Martinez²

2016

AJAC

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Colloid centrifugation can be used to separate heterogeneous populations of cells particularly semen samples, which typically contain mixtures of spermatozoa at different stage of maturity together with dead and dying spermatozoa and also non-sperm cells. Colloids can be used to enrich the population of spermatozoa that are viable and functional; these are the spermatozoa that are needed for fertilization in Assisted Reproduction. Sperm samples obtained after colloid centrifugation may be enriched for motile, morphologically normal spermatozoa with intact chromatin. Insemination of mares with stallion sperm samples prepared by colloid centrifugation resulted in a higher pregnancy rate per cycle than controls, confirming that the good sperm attributes observed in the laboratory were indicative of functional spermatozoa. The method does not simply enrich for viable spermatozoa by inhibiting dead or dying spermatozoa from passing through the colloid since emerging evidence shows that colloid centrifugation can select for spermatozoa with certain properties such as metabolic activity. Other reproductive cells, such as spermatids or progenitor cells, may also be purified by colloid centrifugation. This review describes the history of colloid centrifugation for selecting spermatozoa, compares colloid centrifugation with alternative techniques, and finally describes some of the applications of the technique within the animal breeding industry.

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Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure

Cited by 12 publications

References 23 publications

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Sperm selection in IVF: the long and winding road from bench to bedside

Colloid Centrifugation of Semen: Applications in Assisted Reproduction

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