Introduction: The lack of a Palmaris longus tendon and the absence of a fifth superficial flexor function are normal variations found in the human wrist and hand. The purpose of our study was to assess the incidence of both of these variations and also to answer the question of whether they are evolutionarily conserved muscles. Methods: The study population was volunteers (or informed consent from parents) from preschools, grammar schools, high schools and universities, offices, and nursing homes who were examined for the presence of the Palmaris longus tendon and for variations of the fifth superficial flexor. Results: By the end of the study, 1180 individuals were enrolled and their data were recorded. Of the total 2,360 hands studied, 1,688 had a Palmaris longus tendon (71.4%) and 1,284 had a functional fifth superficial flexor (54.4%). In 886 hands fourth flexor-dependent function was found (37.5%) and ultimately in 190 (8%) the function was absent. Presence, dependent function, or absence of fifth flexor or Palmaris was not related to age, hand side or dominance. Fifth flexor variations were not related to sex, but unilateral absence of Palmaris longus was 1.5 times more common in women. No difference was found between the sexes regarding variations of the superficial flexor. No correlation was found among the variations between one tendon and the other within one hand and/or that individual's other hand. Conclusion: The findings of the present study are similar to those found in some other similar reports related to variations in these tendons. It seems that Palmaris longus and the fifth superficial flexor function are not evolutionarily determined, at least in the studied population.