Frequency of infection with Mycoplasma suis in gestating sows using qPCR on ten commercial French herds, and impact of the infection on clinical, haematological and biochemical parameters

Brissonnier, Mathieu; Normand, Valérie; Lebret, Arnaud; Moalic, Pierre-Yves; Guyomard, Anne-Sophie; Bachy, Véronique; Berton, Pauline; Bouchet, Franck; Boulbria, Gwenaël

doi:10.1186/s40813-020-00152-4

Cited by 11 publications

(11 citation statements)

References 15 publications

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“…(2019) also reported that M. suis -positive farms had significantly more stillborn piglets per litter than negative farms. Similarly, Brissonnier et al. (2020) observed an increase in stillbirths’ rate in gilts positive for M. suis .…”

Section: Discussionmentioning

confidence: 66%

“…Mycoplasma suis is the most common species affecting pigs, which presents itself under the clinical or sub clinical form ( Hoelzle, Zeder, Felder & Hoelzle, 2014 ; Stadler et al., 2014 ). The clinical signs include icterus anemia, fever, and decrease of reproductive performance, leading to increased stillbirth rates and dysgalactia ( Brissonnier et al., 2020 ; Henry, 1979 ; Strait, Hawkins & Wilson, 2012 ). On the other hand, chronic or sub clinically infected animals do not show specific signs, contributing to the spread of the disease and its underdiagnosis ( Ritzmann, Grimm, Heinritzi, Hoelzle & Hoelzle, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of hemotropic Mycoplasmas in fetuses and sows with reproductive failure

Bordin

Gava

Sonálio

et al. 2021

Veterinary and Animal Science

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Investigation of hemotropic Mycoplasmas in fetuses and sows with reproductive failure

Bordin

Gava

Sonálio

et al. 2021

Veterinary and Animal Science

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“…M. haemosuis' was detected in obviously healthy pigs as it was also shown for M. suis in recent studies [13,17,35]. Typically, such chronic HM infections predominate in the pig population causing significant economic loss and welfare concern due to immune dysregulation, higher susceptibility to other infectious agents, extended feeding periods or increased stillbirth rates [4,13,17,35,36]. In addition, chronic HM infections can lead to increased and metaphylactic antibiotic usage contributing to the development of antibiotic resistance [36].…”

Section: Discussionmentioning

confidence: 69%

Occurrence of ‘Candidatus Mycoplasma haemosuis’ in fattening pigs, sows and piglets in Germany using a novel gap-based quantitative real-time PCR assay

et al. 2022

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Background The appearance of the novel porcine haemotrophic mycoplasma (HM) species ‘Candidatus Mycoplasma haemosuis’ was reported in apparently healthy but also in clinically sick animals in China, Korea and in a case report from Germany. Outside of Asia, however, nothing further is known about the frequency of ‘Ca. M. haemosuis’ in pigs to date. To investigate the distribution of this novel HM species in Germany, fattening pigs, sows and pre-suckling piglets were examined using a herein developed quantitative real-time PCR assay (qPCR). Because the piglets were sampled before the first colostrum uptake, additional information on a possible vertical transmission from dams to their offspring was obtained. Results Our novel qPCR assay successfully detected ‘Ca. M. haemosuis’ in all blood samples from the ‘Ca. M. haemosuis’-infected pigs. No cross-reactivity was detected when DNA from non-target Mycoplasma spp. and other bacterial species representing 105 bacteria/reaction were used as a template. The lower limit of detection of the qPCR was thus 10 gap gene copies per reaction and 2.5 x 103 genome equivalents (GE) per mL blood. ‘Candidatus M. haemosuis’ was detected by this qPCR in blood samples from a total out of 6.25% sows (13/208), 4.50% pre-suckling piglets (28/622) and 17.50% fattening pigs (35/200). On farm level, 3 out of 21 piglet producing farms (14.28%) and 9 out of 20 fattening farms (45.00%) were positive for ‘Ca. M. haemosuis’. Co-infections with M. suis were evident in all age groups. Conclusion ‘Candidatus M. haemosuis’ infection is present in German pig farms and the detection of the novel porcine HM species in piglets immediately after birth before colostrum intake indicates vertical transmission. The novel qPCR assay specific for ‘Ca. M. haemosuis’ described herein will be a prerequisite for future studies on the prevalence, epidemiology as well as the clinical and economic impact of ‘Ca. M. haemosuis’ infections.

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“…The PCR negative blood sample results of the 183 investigated boars from 26 different multiplier farms was somehow unexpected, as previous studies revealed a high prevalence of M. suis in sows [25][26][27][28]. However, it might be assumed that multiplier farms have a lower risk of M. suis introduction due the very limited purchase of animals and strict biosecurity measures.…”

Section: Discussionmentioning

confidence: 96%

Update on shedding and transmission routes of porcine haemotrophic mycoplasmas in naturally and experimentally infected pigs

Ade

Ritzmann²,

Wöstmann³

et al. 2021

Porc Health Manag

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Horizontal transmission of Mycoplasma suis via parenteral exposure during standard practices or through bites during fightings have been identified as key epidemiological routes. However, as knowledge gaps on other potential shedding and transmission routes exist, the present study combines both laboratory experiments and field surveys to gain new insights into the epidemiology of porcine haemotrophic mycoplasmas. Splenectomised pigs were orally inoculated with a M. suis field strain and investigated for clinical signs related to infectious anaemia of pigs (IAP) and the presence of M. suis in blood, urine and saliva samples by qPCR. All blood samples were negative for M. suis and animals did not show obvious clinical signs of IAP throughout the entire study period. Additionally, urine, nasal and saliva samples from sows of conventional piglet producing farms and semen samples from a boar stud revealed no detection of M. suis and ‘Candidatus Mycoplasma haemosuis’ by qPCR. Thus, the results indicate that blood-independent transmission routes might be of minor relevance under field conditions.

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Frequency of infection with Mycoplasma suis in gestating sows using qPCR on ten commercial French herds, and impact of the infection on clinical, haematological and biochemical parameters

Cited by 11 publications

References 15 publications

Investigation of hemotropic Mycoplasmas in fetuses and sows with reproductive failure

Investigation of hemotropic Mycoplasmas in fetuses and sows with reproductive failure

Occurrence of ‘Candidatus Mycoplasma haemosuis’ in fattening pigs, sows and piglets in Germany using a novel gap-based quantitative real-time PCR assay

Update on shedding and transmission routes of porcine haemotrophic mycoplasmas in naturally and experimentally infected pigs

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