Frequency of horizontal and vertical transmission for Sarcocystis cruzi and Neospora caninum in dairy cattle

Moré, Gastón; Bacigalupe, D.; Basso, Walter; Rambeaud, Magdalena; Beltrame, F.; Ramirez, Brett C.; Venturini, Marı́a Cecilia; Venturini, L.

doi:10.1016/j.vetpar.2008.10.081

Cited by 49 publications

(36 citation statements)

References 14 publications

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“…Fluctuations in the results similar to these have previously been described (McINNES et al, 2006;DIJKSTRA et al, 2008;MORÉ et al, 2009;YAO et al, 2009;MARQUES et al, 2011). McInnes et al (2006) reported that there was no correlation between the serological tests on dams and the PCR on their fetuses, and a high percentage of infected fetuses had serologically negative dams.…”

Section: Discussionsupporting

confidence: 72%

“…McInnes et al (2006) reported that there was no correlation between the serological tests on dams and the PCR on their fetuses, and a high percentage of infected fetuses had serologically negative dams. Moré et al (2009) showed that transplacental infection in dairy cattle may occur when antibody titers are low in adult cows. Cabral et al (2009) used histology, immunohistochemistry and nested-PCR to evaluate 105 aborted fetuses that were sent to the Biological Institute of São Paulo, Brazil, and found a neosporosis rate of 24.8%.…”

Section: Discussionmentioning

confidence: 99%

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Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Macedo

Cardim

et al. 2013

Rev. Bras. Parasitol. Vet.

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Neospora caninum is a worldwide parasite recognized as one of the main parasites responsible for abortion in cattle. The objective of this study was to evaluate vertical transmission of N. caninum in dairy cows (Bos taurus) that were slaughtered at an abattoir in the state of Santa Catarina, southern Brazil. Blood samples (with and without EDTA) from 60 pregnant dairy cows and blood and tissue samples (brain, lung, heart and liver) from their fetuses were collected and used for PCR and serological evaluation. Blood samples from 60 non-pregnant cows were collected and used to detect antibodies. Anti-N. caninum antibodies were detected by indirect ELISA. Antibodies against N. caninum were observed in 41.6% (25/60) of the pregnant cows and in 43.3% (26/60) of the non-pregnant cows. Antibodies against the parasite were detected in sera from three fetuses (5.5%). PCR analysis revealed that 3.3% (2/60) of the cows and 6.6% (4/60) of the fetuses evaluated were positive for specific N. caninum primers. These positive fetuses were between 4-6 months of age. Therefore, considering PCR and serological tests to be indicative of vertical transmission in fetuses, 11.6% (7/60) of the fetuses were infected by N. caninum during gestation.Keywords: Neospora caninum, dairy cattle, vertical transmission, Bos taurus. ResumoNeospora caninum é um parasita de distribuição mundial reconhecido como um dentre os principais parasitas, responsável por abortamento em bovinos. O objetivo deste estudo foi avaliar a transmissão vertical de N. caninum em vacas leiteiras (Bos taurus) que foram submetidas ao abate em matadouro no Estado de Santa Catarina, sul do Brasil. Sangue (com e sem EDTA) de 60 vacas leiteiras prenhas e amostras de sangue e tecidos (cérebro, pulmão, coração e fígado) de seus fetos foram coletados e utilizados para PCR e avaliação sorológica. Amostras de sangue de 60 vacas não-gestantes foram obtidas e utilizadas para detecção de anticorpos. A detecção de anticorpos séricos anti-N. caninum foi avaliada pelo ELISA-teste indireto. Anticorpos anti-N. caninum foram observados em 41,6% (25/60) das vacas prenhas e em 43,3% (26/60) das vacas não-gestantes. Três fetos (5,5%) foram soros positivos para N. caninum. Análise pela PCR revelou que 3,3% (2/60) das vacas e 6,6% (4/60) dos fetos avaliados foram positivos para N. caninum. As idades dos fetos positivos eram de 4 a 6 meses. Portanto, considerando a PCR e a sorologia como indicativo de transmissão vertical em fetos, 11,6% (7/60) dos fetos foram infectados por N. caninum durante a gestação.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Macedo

Cardim

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…The frequency of vertical transmission on farms A and B was lower than what was reported in Argentina (37.1%) (MORÉ et al, 2009), and this can be partly explained by the size of the sample and the methodology used. To estimate the vertical transmission, the proportion of positive female calves born from mothers that were positive for N. caninum was calculated.…”

Section: Introductioncontrasting

confidence: 63%

Quantification of vertical transmission of Neospora caninum in dairy cows in Minas Gerais, Brazil

Santos

Rocha

Gonçalves

et al. 2012

Rev. Bras. Parasitol. Vet.

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The objective of this cross-sectional study was to estimate the rate of vertical transmission and to investigate horizontal transmission of Neospora caninum and occurrences of reproductive abnormalities in seropositive dairy cows on two farms in the state of Minas Gerais, Brazil. The frequency of cows seropositive for N. caninum according to the indirect fluorescent antibody test (IFAT) was 39.4% (93/236) for Farm A and 31.4% (32/102) for the Farm B (p > 0.05). The mean vertical transmission rates for N. caninum were 29% and 9% for the herds of Farms A and B, respectively. No negative effects (p > 0.05) from infection by N. caninum were observed regarding milk production and occurrences of reproductive abnormalities in herds A and B.

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“…Entretanto, taxa de transmissão vertical menor que 73% foram relatadas em estudos transversais, que não usaram amostras de sangue pré-colostrais (MAINAR-JAIME et al 1999;DYER et al, 2000;BERGERON et al, 2000;ATKINSON et al, 2000;DIJKSTRA et al, 2003;FRANKENA, 2003;PAN et al, 2004;BARTELS et al, 2007;CHANLUN et al, 2007;DIJKSTRA et al, 2008;MORÉ et al, 2009MORÉ et al, , 2010. A mais baixa taxa de transmissão vertical nestes estudos pode ser resultado da ocorrência de transmissão horizontal na vaca ou de seu bezerro após o parto ou declínio do título de anticorpos contra N. caninum abaixo dos níveis de detecção do teste empregado na data do sorodiagnóstico.…”

Section: Transmissão Verticalunclassified

“…Taxas de conversão de animais soropositivos para soronegativos acima de 19% foram relatadas (SAGER et al, 2001;WALDNER et al, 2001a;PAN et al, 2004;CHANLUN et al, 2007;DIJKSTRA et al, 2008;MORÉ et al, 2009). Dijkstra et al (2003) (HO et al, 1997;DAVISON et al, 2001).…”

Section: Transmissão Horizontalunclassified

Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

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Frequency of horizontal and vertical transmission for Sarcocystis cruzi and Neospora caninum in dairy cattle

Cited by 49 publications

References 14 publications

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Quantification of vertical transmission of Neospora caninum in dairy cows in Minas Gerais, Brazil

Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

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