Frequency of HER2/neu overexpression in adenocarcinoma of the gastrointestinal system

Farzand, Sadaf; Siddique, Tahir; Saba, Kanwal; Bukhari, Mulazim Hussain

doi:10.3748/wjg.v20.i19.5889

Cited by 18 publications

(19 citation statements)

References 26 publications

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“…In our series we found 26.6 % positivity for membranous Her-2/ neu staining. On contrary to this, two studies from the same region observed higher percentage of membranous expression of Her-2/ neu in colorectal carcinomas, 74.1 % ( n = 31) and 42 % ( n = 100) respectively [13, 22, 23]. The reason for the difference in the rate might be due to different scoring protocols where they took into account only the membranous expression of Her-2 /neu staining and did not consider the cytoplasmic expression.…”

Section: Discussionmentioning

confidence: 85%

Frequency of Her2/neu expression in colorectal adenocarcinoma: a study from developing South Asian Country

et al. 2016

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BackgroundHuman Epidermal Growth Factor (Her-2/neu) has strong therapeutic implications in certain cancers like breast and gastric cancer. Literature on its frequency in colorectal cancer is scarce. In this study, we have investigated the frequency of Her-2/neu expression in colorectal adenocarcinomas and its association with various clinicopathological variables.MethodsA total of 95 patients who underwent colonoscopic biopsy or colectomy were studied after Institutional Ethical Approval. Hematoxylin & eosin (H&E) staining was performed on all the tissue sections. Expression of Her-2/neu was investigated by immunohistochemistry using α-Her-2 antibody. In order to quantify Her-2/neu expression, three criterias were applied that includes the pattern of staining, intensity of staining and percentage of tumor cells stained. Furthermore, its association was seen with various clinicopathological variables including age, gender, histopathological type, grade and stage of the tumor. Data was entered and analyzed using SPSS version 21. A p-value of < 0.05 was considered as significant.ResultsFrom the total of 95 cases, 75 (78.9 %) cases showed Her-2/neu expression. Pattern of Her-2/neu staining was significantly associated with the grade of colorectal cancer depicting cytoplasmic Her-2/neu expression higher in low grade (50 %) while membranous Her-2/neu expression more in high grade colorectal cancer (45 %) (P-value = 0.030). Pattern of Her-2/neu staining was also significantly associated with the type of colorectal cancer representing membranous Her-2/neu expression to be more common in mucinous type (38.5 %) while cytoplasmic Her-2/neu expression to be more frequent in non mucinous type (42.7 %) of colorectal cancer (p-value = 0.024).We observed a significant association between percentage of cells stained & tumor type, with score 3+ maximum in non mucinous type of colorectal cancer (p-value = 0.006).ConclusionHer2/neu is considerably expressed in colorectal adenocarcinoma in Pakistani population. Our findings indicate a significant strong association of cytoplasmic Her-2/neu expression with low grades and membranous Her-2/neu expression with high grades of colorectal cancer. These findings add to the body of information & may help in conducting clinical trials in future to explore its therapeutic significance as well.

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Section: Discussionmentioning

confidence: 85%

Frequency of Her2/neu expression in colorectal adenocarcinoma: a study from developing South Asian Country

et al. 2016

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“…Moreover, a very few studies have been published from this region regarding Her-2/neu expression in colorectal adenocarcinoma [13][14][15]. Therefore, in this study we attempted to know the expression of Her-2/neu in colorectal tumors in our population which might prove in future a useful prognostic factor and worthy therapeutic target for colorectal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Frequency of Her2/neu expression in colorectal adenocarcinoma: A study from developing South Asian country

Shabbir¹,

Mirza²,

Khalid³

et al. 2016

J Gastrointest Dig Syst

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Background: Human Epidermal Growth Factor (Her-2/neu) has strong therapeutic implications in certain cancers like breast and gastric cancer. Literature on its frequency in colorectal cancer is scarce. In this study, we have investigated the frequency of Her-2/neu expression in colorectal adenocarcinomas and its association with various clinicopathological variables. Methods: A total of 95 patients who underwent colonoscopic biopsy or colectomy were studied after Institutional Ethical Approval. Hematoxylin & eosin (H&E) staining was performed on all the tissue sections. Expression of Her-2/ neu was investigated by immunohistochemistry using α-Her-2 antibody. In order to quantify Her-2/neu expression, three criterias were applied that includes the pattern of staining, intensity of staining and percentage of tumor cells stained. Furthermore, its association was seen with various clinicopathological variables including age, gender, histopathological type, grade and stage of the tumor. Data was entered and analyzed using SPSS version 21. A pvalue of < 0.05 was considered as significant. Results: From the total of 95 cases, 75 (78.9 %) cases showed Her-2/neu expression. Pattern of Her-2/neu staining was significantly associated with the grade of colorectal cancer depicting cytoplasmic Her-2/neu expression higher in low grade (50 %) while membranous Her-2/neu expression more in high grade colorectal cancer (45 %) (P-value = 0.030). Pattern of Her-2/neu staining was also significantly associated with the type of colorectal cancer representing membranous Her-2/neu expression to be more common in mucinous type (38.5 %) while cytoplasmic Her-2/neu expression to be more frequent in non mucinous type (42.7 %) of colorectal cancer (p-value = 0.024). We observed a significant association between percentage of cells stained & tumor type, with score 3+ maximum in non mucinous type of colorectal cancer (p-value = 0.006). Conclusion: Her2/neu is considerably expressed in colorectal adenocarcinoma in Pakistani population. Our findings indicate a significant strong association of cytoplasmic Her-2/neu expression with low grades and membranous Her-2/ neu expression with high grades of colorectal cancer. These findings add to the body of information & may help in conducting clinical trials in future to explore its therapeutic significance as well.

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“…Although cytoplasmic expression of Her/2 has been stated in many other tumors as colon adenocarcinoma [23] and Ewing's sarcoma [24], possible correlation of this expression with patient endurance and response to therapy has not been evaluated. Wilms' tumor is a curable disease and more than 90% of patients live 4 years after diagnosis [25] [26].…”

Section: Discussionmentioning

confidence: 99%

Prognostic Impact of HER/2 Expression on Survival of Preoperatively Treated Children with Wilms Tumor at South Egypt

Sayed¹,

Refaiy²,

Salem³

2017

JCT

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Aim: Wilms tumor (WT) is the most frequent type of pediatric renal tumors. Her/2 is an oncoprotein, its over-expression revealed to play a very vital role in the progress and improvement of certain tumors. This study evaluates the possible role of Her/2 as a prognostic indicator in formerly treated WT. Method: Immunohistochemical expression of Her/2 was studied in paraffin material of 40 WT patients followed SIOP 9 protocol. Patients' medical records reviewed for clinical, pathological and outcome data and correlated with HER2 expression. Additional 15 samples of normal surrounding kidney tissue specimens were included. Results: Her/2 was often expressed in normal kidney tissue (renal tubules but not glomeruli) and at variable levels in the three elements of WT. At a median of 84 months, 70% of patients are living and under follow-up, surgical stage and pathologic subtypes were the only two factors significantly affect the outcome of our patients (p = 0.000, p = 0.007 & p = 0.004, p = 0.005 for OS (Overall survival) and DFS (Disease Free survival) respectively). Her/2 expression was associated with epithelial differentiation (p < 0.001). There was non-significant effect of Her/2 expression on OS or DFS of studied group. Conclusion: while the major progress in studying biology of WT, stage and pathological subtype continues the only predictive factors of significant value affecting the outcome of patients with WT. There was important association between Her/2 expression and histological differentiation in formerly treated Wilms tumor. Non-conclusive results regarding influence of Her/2 expression on the result of WT patients were found.

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Frequency of HER2/neu overexpression in adenocarcinoma of the gastrointestinal system

Abstract: HER2/neu protein is credibly overexpressed in colon and gastric adenocarcinomas in immunohistochemistry. There is significant association between grade of tumor and HER2/neu protein overexpression.

Cited by 18 publications

References 26 publications

Frequency of Her2/neu expression in colorectal adenocarcinoma: a study from developing South Asian Country

Frequency of Her2/neu expression in colorectal adenocarcinoma: a study from developing South Asian Country

Frequency of Her2/neu expression in colorectal adenocarcinoma: A study from developing South Asian country

Prognostic Impact of HER/2 Expression on Survival of Preoperatively Treated Children with Wilms Tumor at South Egypt

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