Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002

Alfieri, Amauri Alcindo; Parazzi, M. E.; Takiuchi, Elisabete; Médici, Kerlei Cristina

doi:10.1007/s11250-006-4349-9

Cited by 159 publications

(141 citation statements)

References 24 publications

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“…All urine samples were processed for RNA extraction (Alfieri et al, 2006) and then used in RT-PCR assays designed to amplify the 287-base pair (bp) fragment of the CDV nucleoprotein gene . Positive control consisted of viral RNA from a previous case ; nuclease-free water (Invitrogen Corporation, Carlsbad, CA, USA) served as negative controls in all RT-PCR assays.…”

Section: Methodsmentioning

confidence: 99%

Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais

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Santos

Bodnar

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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This study investigated the occurrence of canine distemper virus (CDV) by evaluating the presence of viral RNA within urine samples of dogs from Uberlândia, MG, with clinical manifestations suggestive of infection by CDV by targeting the CDV N gene. Of the clinical samples collected (n=33), CDV viruria was detected in 45.5%. Five dogs died spontaneously; all had characteristic CDV-associated histopathological alterations and demonstrated CDV viruria. Statistical analyses revealed that the age, gender, breed, or the organ system of the dog affected had no influence on the occurrence of canine distemper. Myoclonus and motor incoordination were the most significant neurological manifestations observed. A direct association was observed between keratoconjunctivitis and dogs with CDV viruria. These findings suggest that CDV viruria in symptomatic dogs might not be age related, and that symptomatic dogs can demonstrate clinical manifestations attributed to CDV without viruria identified by RT-PCR. Additionally, the results of the sequence identities analysed have suggested that all Brazilian wild-type strains of CDV currently identified are closely related and probably originated from the same lineage of CDV. Nevertheless, phylogenetic analyses suggest that there are different clusters of wild-type strains of CDV circulating within urban canine populations in Brazil.Keywords: canine distemper, viruria, phylogeny, epidemiology RESUMO A presença do ácido nucleico (RNA) do vírus da cinomose canina (CDV) foi avaliada por meio da amplificação parcial do gene N pela técnica RT-PCR realizada em urina de cães provenientes de

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Section: Methodsmentioning

confidence: 99%

Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais

Headley

Santos

Bodnar

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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“…Viral dsRNA was obtained from the fecal samples using a combination of phenol/chloroform/isoamyl alcohol (25:24:1) and silica/guanidinium isothiocyanate nucleic acid extraction methods, modified as described by Alfieri et al (2006). The OSU strain amplified in MA104 cells was used as the positive control for PoRVA and two porcine fecal samples with electropherotype characteristics of group B or C that were confirmed by nucleotide sequence analysis were used as positive controls for PoRVB and PoRVC, respectively (accession numbers: EF577257 and EU002783) (Médici et al 2010a,b).…”

Section: Methodsmentioning

confidence: 99%

Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections

et al. 2014

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Porcine group A rotavirus (PoRVA) is a major cause of neonatal diarrhea in suckling and recently weaned piglets worldwide. The involvement of non-group A rotavirus in cases of neonatal diarrhea in piglets are sporadic. In Brazil there are no reports of the porcine rotavirus group C (PoRVC) as etiologic agent of the diarrhea outbreaks in piglets. The aim of this study was to describe the identification of rotavirus group C in single and in mixed infection with rotavirus groups A and B in three neonatal diarrhea outbreaks in suckling (≤21-day-old) piglets, with 70% to 80% and 20% to 25% of morbidity and lethality rates, respectively, in three pig herds located in the state of Santa Catarina, Brazil. The diagnosis of PoRV in the diarrheic fecal samples was performed using polyacrylamide gel electrophoresis (PAGE) to identify the presence of porcine rotavirus groups A, B (PoRVB), and C, and by RT-PCR (PoRVA and PoRVC) and semi-nested (SN)-PCR (PoRVB) to partially amplify the VP4 (VP8*)-VP7, NSP2, and VP6 genes of PoRVA, PoRVB, and PoRVC, respectively. One RT-PCR (PoRVA and PoRVC) and SN-PCR (PoRVB) product of each group of rotavirus of each diarrhea outbreak was submitted to nucleotide (nt) sequence analysis. Based on the PAGE technique, 4 (25%) and 1 (6.25%) of the 16 diarrheic fecal samples evaluated in the first outbreak presented PoRVA and PoRVC electropherotype, respectively, and 11 (68.75%) were negative. In the second outbreak, 3 (42.85%) of the 7 fecal samples evaluated presented PoRVA electropherotype, and in 3 (42.85%) and in 1 (14.3%) fecal samples were detected inconclusive and negative results, respectively. Three (30%) of the 10 fecal samples of the third outbreak presented PoRVC electropherotype; 5 (50%) and 2 (20%) samples showed negative and inconclusive results, respectively. Based on the RT-PCR and SN-PCR assays in the first neonatal diarrhea outbreak, PoRVC was detected in 13 (81.2%) of the 16 diarrheic fecal samples evaluated. PoRVC single infection was identified in 4 (25%) of these samples and mixed infections with PoRVA and PoRVB in 9 (56.2%) fecal samples. All of the seven diarrheic fecal samples evaluated from the second neonatal diarrhea outbreak were positive for PoRVC, whereas its mixed infection with other PoRV groups was detected in 4 (57.2%) samples. In the third outbreak, PoRVC in single infection was detected in all of the 10 diarrheic fecal samples analyzed. In the nt sequence analysis, the PoRVA strains of the first and second outbreaks demonstrated higher nt identity with G4P[6] and G9P [23] genotypes, respectively. The PoRVB strains (first and second outbreaks) and the PoRVC

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“…Selected fecal samples (n=12) were also screened for the presence of rotavirus groups A, B and C, and enteric calicivirus (Norovirus and Sapovirus genera) by reverse transcription polymerase chain reaction (RT-PCR) assay. The viral nucleic acid was extracted from 10% to 20% (w/v) fecal suspensions in PBS according to Alfieri et al (2006) followed by RT-PCR with specific primers for G and P genotypes of group A (Gouvea et al 1990, Gentsch et al 1992; group B, which also included a second round of amplification (semi-nested PCR) (Gouvea et al 1991); and group C (Alfieri et al 1999). The RT-PCR for enteric calicivirus was performed in accordance to Barry et al (2008) with primers described by Jiang et al (1999) that detect norovirus and sapovirus strains.…”

Section: Methodsmentioning

confidence: 99%

Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome

et al. 2008

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RESUMO.-[Lesões entéricas em suínos afetados por síndrome multissistêmica do definhamento dos suínos.]Amostras de linfonodos mesentéricos e intestinos de 79 leitões desmamados refugos, entre 3 e 5 meses de idade e confirmados como naturalmente afetados pela síndrome multissistêmica do definhamento foram estudadas. Os suí-nos eram oriundos de 12 criações no sul do país e foram selecionados em função dos sinais clínicos e/ou lesões macroscópicas sugestivos de doença entérica. Infiltrados linfoistiocíticos de intensidades variáveis foram associados com marcação positiva anti-circovirus suíno tipo 2 (anti-PCV2) em amostras de intestinos e linfonodos mesentéri-cos de todos os 79 animais. Embora a maioria dos achados fossem semelhantes aos descritos em enterite associada com PCV2, marcação imuno-histoquímica anti-PCV2 foi associada com depleção de células caliciformes (24 suínos), atrofia e fusão de vilosidades do íleo (18 suínos) e dilatação de vasos linfáticos (11 suínos) combinada ou não com linfangite. Antígenos de PCV2 foram demonstrados por imuno-histoquímica no citoplasma e núcleo de células epiteliais intralesionais, histiócitos e células tipo endotelial Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3-to 5-monthold postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.

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Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002

Cited by 159 publications

References 24 publications

Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais

Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais

Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections

Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome

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