Frequency of different genotypes of Giardia duodenalis in slaughtered sheep and goat in east of iran

Faridi, Ashkan; Kareshk, Amir Tavakoli; Sadooghian, Saeed; Firouzeh, Nima

doi:10.1007/s12639-020-01237-1

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Giardia duodenalis has been widely reported in animals worldwide, with prevalence of 1.6-91.33% [17,19,20,24,25,68]. In this study, a total of 19 rabbit fecal samples were identified as positive for G. duodenalis infection by nested-PCR targeting the bg gene, for a total prevalence of 3.54% (19/537).…”

Section: Discussionmentioning

confidence: 88%

Prevalence and multi-locus genotyping of Giardia duodenalis in rabbits from Shaanxi province in northwestern China

Tang

Kang

et al. 2021

Parasite

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Giardia duodenalis is an important parasite with veterinary and public health significance worldwide. The presence and zoonotic assemblages of G. duodenalis have previously been reported in rabbits. In this study, to understand the infection status of G. duodenalis in rabbits from Shaanxi province, a total of 537 fecal samples were collected from two breeds of rabbits in four age groups (<30 days, 31–90 days, 91–200 days and >200 days) from four geographical origins (Fengxiang, Yangling, Tongchuan, and Shanyang). The presence of G. duodenalis in these samples was assessed using molecular assays based on beta-giardin (bg). The glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) loci were then amplified in the bg-positive samples for multi-locus genotype (MLG) analysis. The total prevalence of G. duodenalis in these rabbits was 3.54% (19/537). Giardia duodenalis infection was found in both breeds of rabbits, and in all farms and age groups, but with no statistically significant differences related to these factors (p > 0.05). Two assemblages, including B and E, were identified, with the former the predominant assemblage detected in both breeds, and in all age groups and farms. Sequence analysis revealed 2 (named as rbg1-2), 1 (named as rtpi1), and 2 (named as rgdh1-2) haplotypes at the gene loci of bg, tpi, and gdh, respectively, forming a multilocus genotype (MLG) of assemblage B (rbg1, rtpi1, and rgdh1). These findings reveal the significant zoonotic potential and genetic diversity of G. duodenalis in rabbits in Shaanxi Province, PR China.

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Section: Discussionmentioning

confidence: 88%

Prevalence and multi-locus genotyping of Giardia duodenalis in rabbits from Shaanxi province in northwestern China

Tang

Kang

et al. 2021

Parasite

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“…If no samples were successfully genotyped based on the originally extracted DNA further isolation of cysts/oocysts was attempted using a sucrose gradient protocol modified from Faridi et al. ( 2020 ) and Lebbad et al. ( 2008 ) and subsequent subtyping.…”

Section: Methodsmentioning

confidence: 99%

“…no samples were successfully genotyped based on the originally extracted DNA further isolation of cysts/oocysts was attempted using a sucrose gradient protocol modified fromFaridi et al (2020) andLebbad et al (2008) and subsequent subtyping. In short, 3 g of faeces were homogenised with 8 ml PBS, and the solution was filtered through a double layer of gauze into a 50-ml tube.…”

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confidence: 99%

Zoonotic pathogens in wild muskoxen (Ovibos moschatus) and domestic sheep (Ovis aries) from Greenland

Berg

Stensvold

Jokelainen

et al. 2021

Veterinary Medicine & Sci

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The present study aimed to estimate the prevalence of zoonotic pathogens Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Erysipelothrix in muskoxen (Ovibos moschatus) and sheep (Ovis aries) from Greenland. In 2017 and 2018, faecal samples were collected from wild muskoxen from three distinct populations (Zackenberg, Kangerlussuaq, and Ivittuut) and from domestic sheep from southwest Greenland.Blood samples were collected from muskoxen from Kangerlussuaq and Ivittuut and from sheep. Faecal samples were tested for specific DNA of G. duodenalis and Cryptosporidium spp., and blood samples were tested for antibodies against T. gondii and Erysipelothrix. The estimated prevalence of G. duodenalis was 0% (0/58), 17% (7/41) and 0% (0/55) in muskoxen from Zackenberg, Kangerlussuaq and Ivittuut, respectively, and 37% (16/43) in sheep. The estimated prevalence of Cryptosporidium was 0% (0/58), 2%(1/41), 7% (4/55) in muskoxen from Zackenberg, Kangerlussuaq, Ivittuut, respectively, and 2% (1/43) in sheep. Neither Giardia nor Cryptosporidium were detected in winter samples (0/78). Of the positive samples, Giardia from one muskox sample only was successfully typed as G. duodenalis assemblage A, and Cryptosporidium from two muskoxen was successfully typed as C. parvum, subtype IIdA20G1e. The estimated T. gondii seroprevalence was 2% (1/44) and 0% (0/8) in muskoxen from Kangerlussuaq and Ivittuut, respectively, and 1% (1/155) in sheep. The estimated Erysipelothrix seroprevalence was 2% (1/45) and 13% (1/8) in muskoxen from Kangerlussuaq and Ivittuut, respectively, and 7% (10/150) in sheep. The results of this study add to the scarce knowledge on zoonotic pathogens in the Arctic.

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“…The results of the studies on Giardia duodenalis positive isolates revealed genotypes A (AI, AII), C, and E were present in sheep and goats. [29][30][31] Also, the study on infected household dogs and cats revealed the presence of genotypes A (AI, AII), C, D, and F. 32…”

Section: Genotypes Of Giardia In Animalsmentioning

confidence: 98%

A review of giardiasis and its parasite genotypes in Iran

Khamesipour¹,

Dehkordi²,

Afzal³

et al. 2021

Infect Dis Herbal Med

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Giardia lamblia is one of the most prevailing intestinal protozoa in many vertebrates like humans, domestic and wild animals. Molecular studies show that Giardia lamblia is a complex parasite. Currently, giardiasis disease is one of the main problems of social and personal health in different countries around the world. Giardia is known as a zoonotic parasite that is divided into eight genetic assemblages (A to H). In this review study, by referring to the molecular epidemiology of Giardia lamblia and emphasizing its zoonotic factors, the background of Giardia and its genotypes in Iran have been studied. The study population consisted of indexed articles in reputable databases such as Scopus, Magiran, SID, Science Direct, PubMed, and Google Scholar in Iran from 1990 to 2020. Based on limited studies in Iran, assemblage BIII and AII are the most common types of Giardia assemblages. Therefore, it is necessary to conduct comprehensive studies on various human and animal isolates in different areas of the country, especially places where no researches have been done.

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Frequency of different genotypes of Giardia duodenalis in slaughtered sheep and goat in east of iran

Cited by 7 publications

References 17 publications

Prevalence and multi-locus genotyping of Giardia duodenalis in rabbits from Shaanxi province in northwestern China

Prevalence and multi-locus genotyping of Giardia duodenalis in rabbits from Shaanxi province in northwestern China

Zoonotic pathogens in wild muskoxen (Ovibos moschatus) and domestic sheep (Ovis aries) from Greenland

A review of giardiasis and its parasite genotypes in Iran

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