Frequency of colonization with “hypervirulent” Klebsiella pneumoniae strains of newborns and infants with community-acquired and nosocomial klebsiella infection

Семенова, Д. Р.; Nikolaeva, I. V.; Fialkina, S V; Хаертынов, Х. С.; Анохин, В. А.; Валиуллина, И. Р.

doi:10.21508/1027-4065-2020-65-5-158-163

Cited by 6 publications

(1 citation statement)

References 18 publications

(23 reference statements)

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“…Внебольничные случаи кишечного клебсиеллеза у детей разного возраста предполагают возможность контактно-бытового и пищевого путей инфицирования [51,52]. Следует, однако, отметить, что в сравнительном аспекте клинико-эпидемиологическая характеристика клебсиеллезной инфекции внебольничного и внутрибольничного происхождения у детей изучена недостаточно [53].…”

Section: Klebsiella Pneumoniae как возбудитель внутрибольничной и вне...unclassified

Colonization Resistance and Intestinal Microbiota as Factors of Counteraction to the Development of Intestinal Infections (Review)

Гончар,

Скрипченко,

Коперсак

2023

Children's Medicine of the North-West

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При сохраняющейся тенденции роста заболеваемости детей острыми кишечными инфекциями в раннем детском возрасте в последние годы сохраняется значение бактериальных возбудителей условно-патогенной природы. Вопросы этиологической и эпидемиологической значимости условно-патогенных энтеробактерий у детей, не имеющих признаков иммунодефицита, остаются нерешенными. Существует множество уровней защиты организма человека от патогенов, которые реализуются за счет прямых механизмов взаимодействия между микробами и косвенных механизмов, опосредованных стимуляцией иммунной системы слизистой оболочки индигенными представителями микробиоты. Бактерио цины комменсальных бактерий могут ингибировать патогенные и условно-патогенные микроорганизмы, участвуя в формировании структуры микробиоты желудочно-кишечного тракта. Колонизационная резистентность слизистых оболочек кишечника и колонизационная активность микробов представляют собой прямо противоположные, но взаимосвязанные процессы. Условно-патогенные энтеробактерии приобретают свойства патогенности и становятся опасными возбудителями инфекционных диарей при определенных условиях, которые создаются при изменении свойств среды. Микробиота кишечника в зависимости от своего состояния активно участвует в предотвращении, но иногда и в провоцировании диарейных заболеваний. В настоящее время среди условно-патогенных возбудителей кишечных инфекций внебольничного происхождения у детей первых трех лет жизни лидирующая роль принадлежит Klebsiella pneumoniae. With the continuing trend of an increase in the incidence of acute intestinal infections in children in early childhood in recent years, the importance of bacterial pathogens of a conditionally pathogenic nature has remained. The issues of etiological and epidemiological signifi cance of conditionally pathogenic enterobacteria in children without signs of immunodefi ciency remain unresolved. There are many levels of protection of the human body from pathogens, which are realized through direct mechanisms of interaction between microbes, and indirect mechanisms mediated by stimulation of the immune system of the mucous membrane by indigenous representatives of the microbiota. Bacteriocins of commensal bacteria can inhibit pathogenic and opportunistic microorganisms, participating in the formation of the structure of the microbiota of the gastrointestinal tract. Colonization resistance of intestinal mucous membranes and colonization activity of microbes are directly opposite, but interrelated processes. Conditionally pathogenic enterobacteria acquire pathogenicity properties and become dangerous pathogens of infectious diarrhea under certain conditions that are created when the properties of the environment change. The intestinal microbiota, depending on its condition, is actively involved in the prevention, but sometimes also in provoking diarrheal diseases. Currently, Klebsiella pneumoniae plays a leading role among the opportunistic pathogens of intestinal infections of community-acquired origin in children of the fi rst three years of life.

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Section: Klebsiella Pneumoniae как возбудитель внутрибольничной и вне...unclassified

Colonization Resistance and Intestinal Microbiota as Factors of Counteraction to the Development of Intestinal Infections (Review)

Гончар,

Скрипченко,

Коперсак

2023

Children's Medicine of the North-West

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ESCHERICHIA COLI phenotypic characteristics and antagonistic activity in opisthorchiasis invasion

Kataeva,

Karpukhina,

Vakarina

et al. 2024

Russian Journal of Infection and Immunity

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Opisthorchis felineus invasion in human causes inflammatory and dyskinetic disorders of the gastrointestinal tract accompanied by altered phenotypic characteristics in colon microbiota. The aim of research — study an impact of the Escherichia coli isolate phenotypic characteristics on Klebsiella spp. bacteria, isolated from colonic contents of patients with diagnosed opisthorchiasis as well as E. coli antagonistic activity. Materials and methods. The phenotypic properties of 54 E. coli isolates and 8 genus Klebsiella isolates obtained from colonic contents of patients with diagnosed opisthorchiasis were assessed. Identification of isolates and analysis of proteomic profiles were performed using Maldi BioTyper 3.0 software. 204 co-cultivation datasets were analyzed investigating antagonistic activity of E. coli isolates with varying properties on Klebsiella spp. E. coli and Klebsiella spp. isolates were examined by whole genome sequencing. Results. E. coli bacteria with typical phenotypic characteristics showed significantly more prominent antagonistic activity against Klebsiella spp. A significantly higher level of antagonistic activity against K. oxytoca bacteria vs K. pneumoniae strains. The proteomic bacterial strain profiles were divided into clusters depending on the level of antagonistic activity. E. coli molecular serotyping for O- and H-antigens revealed the genes of enterotoxigenic, enteroinvasive and extraintestinal pathogens in 60.0% of cases. Strains with the highest antagonistic activity index, which are carriers of the genes typical to enterotoxigenic E. coli sequence serotypes O6:H1 and O6:H5, were identified. The genome of such strains consisted of the largest number of virulence gene complexes: adhesins, invasins, toxins, bacteriocins. Multilocus sequence typing and sequence serotyping of E. coli and K. pneumoniae strains established their heterogeneity; K. oxytoca isolates were identified as ST242 and ST176. All strains were characterized by homology of antibiotic resistance markers (oqxA, oqxB, fosA) and a variety of beta-lactam resistance gene variants. Conclusion. It was found that E. coli isolates with typical phenotypic characteristics and carriers of virulence gene complexes exhibited significantly more pronounced antagonistic activity against Klebsiella spp. isolated from colonic contents of patients with diagnosed opisthorchiasis.

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Microbiological and molecular genetic characteristics of Klebsiella pneumoniae isolates, extracted under conditions of cardiac surgery hospital

Kuznetsova,

Sergevnin,

Mikhailovskaya

et al. 2024

Russian Journal of Infection and Immunity

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Klebsiella pneumoniae is one of the main causative agents of healthcare-associated infections (HAI) worldwide. The study was aimed at molecular characterization of beta-lactam antibiotic resistance in K. pneumoniae isolated in the cardiac surgery hospital, identify hypervirulent isolates and assess isolate genetic relatedness. K. pneumoniae isolated from the clinical material of patients (n = 50) at cardiac surgery departments as well as anesthesiology and reanimation department treated at the cardiovascular surgery hospital in Perm from July 2021 to December 2022 were analyzed. Bacteriological studies were performed on an automatic analyzer WalkAway-96Plus (Beckman Coulter, USA). The sensitivity to thirteen antimicrobials, production of extended-spectrum beta-lactamases (ESBL) and multidrug resistance (MDR) phenotype were assessed. The string test was used to screen hypermucoviscous K. pneumoniae. Isolate relatedness (ERIC-PCR), presence of beta-lactamase genes (blaTEM, blaCTX-M, blaSHV, blaOXA, blaKPC, blaVIM-2, blaIMP-1, blaNDM-1), as well as genes associated with hypervirulence (prmpA, iucA, peg-344, wzyK2, magA) were analyzed by PCR. Amplifications were performed in a DNA Engine Dyad Thermal Cycler (Bio-Rad, USA). Gel documentation system Gel-Doc XR (Bio-Rad, USA) was used for band visualization and data documentation. According to the study results, it was found that carbapenem-resistant isolates accounted for almost 76% of all isolates, the ESBL-phenotype had 90%, and MDR-phenotype — 88% of studied isolates. According to the string test results, 8% of K. pneumoniae isolates had a hypermucoviscous phenotype. As for genes associated with hypervirulence, it was shown that the iucA gene was the most common, amplified in 54% of isolates, prmpA was detected in 38% of isolates, 48% of isolates had wzyK2 or magA genes encoding capsular type K1/K2. Almost a third of K. pneumoniae samples contained a combination of the rmpA and iucA genes, and they were isolated more often in the group of patients with active infection. Only carbapenem-sensitive microorganisms had hypermucoviscous phenotype, K1 capsular type, and a combination of prmpA, iucA, peg-344 genes, whereas the K2 capsular type was most common among carbapenem-resistant K. pneumoniae. Based on molecular genetic typing, it was found that 24% of K. pneumoniae isolates were assigned to four genome groups of identical cultures, 76% of the isolates had individual profiles. Closely related isolates were found among patients from different departments that indicates a potential pathogen circulation in the closed circuit “surgery department–department of anesthesiology” and “reanimation–surgery department”.

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Frequency of colonization with “hypervirulent” Klebsiella pneumoniae strains of newborns and infants with community-acquired and nosocomial klebsiella infection

Cited by 6 publications

References 18 publications

Colonization Resistance and Intestinal Microbiota as Factors of Counteraction to the Development of Intestinal Infections (Review)

Colonization Resistance and Intestinal Microbiota as Factors of Counteraction to the Development of Intestinal Infections (Review)

ESCHERICHIA COLI phenotypic characteristics and antagonistic activity in opisthorchiasis invasion

Microbiological and molecular genetic characteristics of Klebsiella pneumoniae isolates, extracted under conditions of cardiac surgery hospital

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