Frequency of anti-hsp60, -65 and -70 antibodies in sera of patients with juvenile idiopathic arthritis

Zlacka, Denisa; Vavrincová, P; Nguyen, Thị Thu Hien; Hromadníková, Ilona

doi:10.1016/j.jaut.2006.06.006

Cited by 39 publications

(25 citation statements)

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“…The above correlations support suggestion that Hsp40s may participate in the pathomechanisms of RA in humans. A correlation between the level of humoral response and Steinbrocker's stages was demonstrated previously for another class of Hsps, the Hsp70 (Zlacka et al 2006).…”

Section: Discussionsupporting

confidence: 68%

“…Recently, a number of studies have investigated a role of the heat shock proteins (Hsps) in T-cell regulation in chronic inflammatory diseases and their outcome indicates that T-cell responses to Hsps have disease-suppressive activities through production of anti-inflammatory cytokines (van Eden et al 2005;Zlacka et al 2006;Wieten et al 2007;van Eden et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Hsp40 proteins modulate humoral and cellular immune response in rheumatoid arthritis patients

Tukaj

Kotlarz

Jóźwik

et al. 2010

Cell Stress and Chaperones

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Recent research on the heat shock proteins (Hsps) in chronic inflammatory diseases indicates that Hsps may have disease-suppressive activities. Our aim was to characterize immune response directed to bacterial (DnaJ) and human Hsp40s in patients with rheumatoid arthritis (RA). We found elevated levels of anti-DnaJ, anti-Hdj2, and anti-Hdj3 (but not ant-Hdj1) serum antibodies in the RA patients (P < or = 0.001) compared to healthy controls. In peripheral blood mononuclear cells (PBMCs) culture, all tested Hsp40 proteins significantly inhibited the divisions of CD4+ and CD8+ T cells of the RA patients but not those of the controls. Both DnaJ and Hdj2 stimulated secretion of the main anti-inflammatory cytokine IL-10 by PBMCs of the RA patients (P < 0.05), and of IL-6 by PBMCs of the RA (P < 0.001) and control (P < 0.01) groups. DnaJ reduced TNFalpha secretion (P < 0.05) by both groups of PBMCs. Our results show for the first time that the RA patients have an increased humoral response to human Hsp40 proteins Hdj2 and Hdj3. This is also the first description of immunomodulatory effect of human Hsp40s on T cells and cytokine secretion in RA, suggesting that Hsp40s act as natural anti-inflammatory agents in RA.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Hsp40 proteins modulate humoral and cellular immune response in rheumatoid arthritis patients

Tukaj

Kotlarz

Jóźwik

et al. 2010

Cell Stress and Chaperones

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“…Antibodies targeting HSP-70 have been detected in sera from patients with sensory neuronal hearing loss [7], systemic lupus erythematosus [8], and juvenile idiopathic arthritis [9]. When applying HSP-70 antibodies together with an agent inducing allergic dermatitis, mice did not develop the disease but gained systemic antigen-specific tolerance, which could also be adoptively transferred [10].…”

Section: Heat-shock Proteinsmentioning

confidence: 99%

Heat-Shock Proteins in Clinical Neurology

2011

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Heat-shock proteins (HSPs) are antigen-presenting protein-aggregation-preventing chaperones, induced by cellular stress in eukaryotic cells. In this review, we focus on recent HSP advances in neurological disorders. In myasthenia gravis, patients responding to immunosuppressive therapy have reduced serum HSP-71 antibodies. Generalized and ocular myasthenia gravis patients have elevated serum HSP-70 antibodies, indicating common pathogenic mechanisms. In Guillain-Barré syndrome, HSP-70 antibodies are elevated in serum and cerebrospinal fluid, and serum levels are higher than in myasthenia gravis and multiple sclerosis. In multiple sclerosis, serum HSP-27 antibodies are elevated during relapses providing disease activation marker, while α,β-crystallin expression in brain lesions indicates remission phase initiation. In acute stroke, serum HSP-27 antibodies are elevated irrespective of stroke type and duration. In epilepsy, HSP-27 is induced in patients’ astrocytes and cerebral blood vessel walls, and α,β-crystallin is expressed in epileptic foci. In neurodegenerative disorders such as Alzheimer dementia and Parkinson’s disease, HSPs are upregulated in brain tissue, and α,β-crystallin modulates superoxide dismutase-1 (SOD-1) tissue accumulation in familial amyotrophic lateral sclerosis. HSPs play an important role in antigen-presentation and tolerance development. Antibody-mediated interference with their function alters immune responses causing neuropathology. The role of HSPs in clinical neurology should be the subject of future investigation.

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Section: Introductionmentioning

confidence: 99%

“…Notably, anti-HSP70 autoantibodies have been detected in 94% of patients with esophageal cancer but less commonly in other cancers (11% and 20% for gastric and colon) [25]. Anti-HSP70 antibodies have been detected in subgroups of patients with juvenile idiopathic arthritis [26], multiple sclerosis [27], Graves's disease [28], Behçet's disease [29], and sudden sensorineural hearing loss [30] but the significance of this in these diseases has yet to be determined.In this study we determined that RFs (preferentially IgA) were associated with chronic smoke-induced LD in mice and in two cohorts of smokers, none of whom had an underlying autoimmune disease. In a cohort of patients with an autoimmune disease, namely systemic sclerosis (SSc), we show that whereas lung inflammation in the absence of smoke exposure can lead to an IgM anti-HSP70 autoimmune response but no or very limited RF response, the switch to IgG anti-HSP70 is observed primarily in smokers with LD.…”

mentioning

confidence: 99%

Chronic smoke exposure induces rheumatoid factor and anti‐heat shock protein 70 autoantibodies in susceptible mice and humans with lung disease

et al. 2012

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The impact of cigarette smoke (CS), a risk factor for rheumatoid arthritis (RA), on autoantibody production was studied in humans and mice with and without chronic lung disease (LD). Rheumatoid factor (RF), anti-cyclic citrullinated peptides (CCPs), and anti-HSP70 autoantibodies were measured in several mouse strains and in cohorts of smokers and nonsmokers with and without autoimmune disease. Chronic smoking-induced RFs in AKR/J mice, which are most susceptible to LD. RFs were identified in human smokers, preferentially in those with LD. Anti-HSP70 autoantibodies were identified in CS-exposed AKR/J mice but not in ambient air exposed AKR/J controls. Whereas inflammation could induce anti-HSP70 IgM, smoke exposure promoted the switch to anti-HSP70 IgG autoantibodies. Elevated anti-CCP autoantibodies were not detected in CS-exposed mice or smokers. AKR/J splenocytes stimulated in vitro by immune complexes (ICs) of HSP70/anti-HSP70 antibodies produced RFs. The CD91 scavenger pathway was required as anti-CD91 blocked the HSP70-IC-induced RF response. Blocking Toll-like receptors did not influence the HSP70-IC-induced RFs. These studies identify both anti-HSP70 and RFs as serological markers of smoke-related LD in humans and mice. Identification of these autoantibodies could suggest a common environmental insult, namely CS, in a number of different disease settings.Keywords: Autoantibodies r Heat shock protein 70 r Lung disease r Rheumatoid factor IntroductionSmoking is a very strong environmental risk factor that is linked to rheumatoid arthritis (RA) [1,2] and other autoimmune disCorrespondence: Dr. Marianna M. Newkirk e-mail: Marianna.newkirk@mcgill.ca eases [3][4][5]. Furthermore, there is little information on the association between the most common smoke-induced lung disease (LD), chronic obstructive pulmonary disease (COPD), and serological markers associated with autoimmunity.In North American Native (NAN) populations the prevalence of RA can be three times that of Caucasians [6]. Whereas smoking rates have decreased in the past decade in Caucasians, with 18% of Canadians >15 years of age smoking, 60-70% C 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu1052 Marianna M. Newkirk et al. Eur. J. Immunol. 2012. 42: 1051-1061 of NANs (residing mostly on reservations) smoke (Health Canada). Although smoking cessation can lower RA risk and disease burden, the risk continues 10 years after cessation [1] and similarly, prolonged smoking cessation is critical for the reduction of lung cancer [7]. Lung inflammation, oxidative stress, and protease burden remain elevated for several months after smoking cessation [8]. Early diagnosis of those at risk of smoking-related diseases ideally by the use of informative biomarkers is critical with sustained cessation an important goal. Rheumatoid factor (RF), used in the diagnosis of RA [9], is an autoantibody of any isotype that binds IgG Fc. Epidemiological studies have shown that RF-positive RA patients are most associated with an increased risk conferred f...

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Frequency of anti-hsp60, -65 and -70 antibodies in sera of patients with juvenile idiopathic arthritis

Cited by 39 publications

References 42 publications

Hsp40 proteins modulate humoral and cellular immune response in rheumatoid arthritis patients

Hsp40 proteins modulate humoral and cellular immune response in rheumatoid arthritis patients

Heat-Shock Proteins in Clinical Neurology

Chronic smoke exposure induces rheumatoid factor and anti‐heat shock protein 70 autoantibodies in susceptible mice and humans with lung disease

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