Frequency of ANA/DFS70 in relatives of patients with rheumatoid arthritis compared to patients with rheumatoid arthritis and a healthy population, and its association with health status

Romero-Álvarez, Verónica; Acero-Molina, Diana Alejandra; Beltrán-Ostos, Adriana; Bello-Gualtero, Juan Manuel; Romero-Sánchez, Consuelo

doi:10.1016/j.reumae.2019.02.008

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…In a recent study conducted in Spain, the anti-DFS70 positivity rate in RA patients was 1.66% when all patients were taken into account, while the positivity rate was 12.9% among only ANA-positive patients [21]. In another study from Japan, the anti-DFS70 positivity rate was found to be 18.1% using Enzyme-linked immunosorbent assay and IIF methods in RA patients [22].…”

Section: Discussionmentioning

confidence: 95%

Anti-dense fine speckled (DFS) antibody: its staining pattern in indirect immunofluorescence and its clinical relevance

Karahan

Emre

Hörmet-Öz

2022

Romanian Journal of Internal Medicine

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Background: Dense fine speckled (DFS) pattern defined by very intense, heterogeneous speckled staining of nucleoplasms of interphase HEp-2 cells and chromosomal areas of metaphase cells. The association of Anti-DFS70 and rheumatologic signs, symptoms, and diagnosis were evaluated. Methods: One-hundred-eight anti-DFS70 positives who consecutively admitted Rheumatology clinic between January-June 2020 were analyzed. The clinical and laboratory findings of positives for anti-DFS70 antibody were compared with those with DFS pattern ANA IFA staining rates. Also, anti-DFS70 positivity rates and their correlation with the DFS staining pattern were analyzed retrospectively in 1016 CTD patients. Results: The most common complaint was joint pain seen in 77(71.3%) and the most common laboratory abnormality was RF-positivity observed in 10/108(9.3%) who had anti-DFS70 positivity. The most common ANA staining pattern was DFS (72/108;66,7%); one-third had other than DFS. No statistical significance was found for the association of any of the rheumatological complaints and laboratory findings with the DFS70 staining pattern. ANA analysis was performed in a total of 964/1016(94.88%) CTD patients and 44 (4.56%) of these positive for anti-DFS70. The correlation coefficient showed good correlations between the DFS pattern staining and anti-DFS70 antibody positivity (r=+0.773, p<0.001). Conclusions: Anti-DFS70-positives have a low rate of CTD. A low anti-DFS70 positivity rate was observed in patients with CTD. As such, it can be considered that anti-DFS70 does not predict CTD or even excludes it.

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Section: Discussionmentioning

confidence: 95%

Anti-dense fine speckled (DFS) antibody: its staining pattern in indirect immunofluorescence and its clinical relevance

Karahan

Emre

Hörmet-Öz

2022

Romanian Journal of Internal Medicine

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“…Comparable to our findings and in the most recent work higher prevalence of ANA was observed in Mexican patients with early RA reaching 25.8% in control individuals. 31 Anti-Ro/SSA and anti-La/SSB autoantibodies recognize different epitopes on small RNAs associated polypeptides. At least two major proteins are identified called Ro/SSA: a 52 kDa (Ro) and a 60 kDa (Ro), while for La/SSB only one protein with 48 kDa.…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies to extractable nuclear antigens (ENAs) pattern in rheumatoid arthritis patients: Relevance and clinical implications

Emad

Ragab

Hammam

et al. 2021

Reumatología Clínica

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“…Circulating autoantibodies to DFS70 may play a protective role against kidney damage in lupus nephritis Greisha L. Ortiz-Hernandez [26] "Monospecific" autoantibodies to DFS70/LEDGF (detectable only by ANA in serum) were not associated with SARD and were found in healthy individuals and some patients with inflammatory conditions not related to SARD Teck Choon Tan [47] 645 Anti-DFS70 was not associated with the absence of SARD Verónica Romero-Álvarez [48] 240…”

Section: Author Number Of Cases Conclusionmentioning

confidence: 99%

The Role of Anti-DFS70 in the Diagnosis of Systemic Autoimmune Rheumatic Diseases

Zotova,

Kotova,

Kuznetsov

2023

Biologics

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The diagnosis of systemic autoimmune rheumatic disease (SARD) or its exclusion is carried out taking into account the results of immunological studies, primarily antinuclear antibodies (ANA) and specific autoantibodies. Often, during ANA analysis via indirect immunofluorescence reaction on cellular and tissue substrates, a dense fine speckled 70 (DFS70) fluorescence pattern is observed. Studies on the diagnostic significance of antibodies to anti-DFS70 allow for optimizing the stepwise diagnosis of SARD. Currently, a two-step strategy for laboratory diagnostic investigation is recommended: in the first step, ANA screening is performed, and in the second step, patients with positive results undergo confirmatory tests to detect specific antibodies against individual nuclear antigens. The detection of anti-DFS70 in ANA-seropositive patients without clinical and/or other specific serological markers characteristic of a particular disease within the SARD group may be considered a negative prognostic marker. Also, in the process of decision making in clinical practice, we should remember that anti-DFS70 can be found in the blood of patients with a different, non-SARD pathology and that most people showing anti-DFS70 are healthy individuals.

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Frequency of ANA/DFS70 in relatives of patients with rheumatoid arthritis compared to patients with rheumatoid arthritis and a healthy population, and its association with health status

Cited by 5 publications

References 31 publications

Anti-dense fine speckled (DFS) antibody: its staining pattern in indirect immunofluorescence and its clinical relevance

Anti-dense fine speckled (DFS) antibody: its staining pattern in indirect immunofluorescence and its clinical relevance

Autoantibodies to extractable nuclear antigens (ENAs) pattern in rheumatoid arthritis patients: Relevance and clinical implications

The Role of Anti-DFS70 in the Diagnosis of Systemic Autoimmune Rheumatic Diseases

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