Frequency of a mutant allele of cytochrome b conferring resistance to Ool fungicides in the Czech population of Ramularia collo-cygni

Matušinsky, Pavel; Svobodova-Leisova, Leona; Mariks, Pavel; Tvarůžek, L.; Stemberková, L.; Hanusová, Martina; Minarikova, V.; Vysohlidova, Marketa; Spitzer, T.

doi:10.1007/bf03356369

Cited by 21 publications

(15 citation statements)

References 10 publications

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“…Mutant and wild alleles of cytochrome b in the Czech population of Rcc were detected at an equal rate (Matusinsky et al. ). Based on this finding, it can be assumed that the rate of resistant Rcc populations will increase with the use of strobilurin fungicides, potentially increasing the incidence of the allele to nearly 100%.…”

Section: Discussionmentioning

confidence: 99%

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Variability of the Ramularia collo‐cygni Population in Central Europe

Leišová‐Svobodová

Matušinsky

Kučera

2012

Journal of Phytopathology

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The genetic structure of the fungal barley pathogen Ramularia collo-cygni (Rcc) population in Central Europe involving the isolates from the Czech Republic, the Slovak Republic, Germany and Swiss was determined using amplified fragment length polymorphism (AFLP) analysis. One hundred and eighty-four markers were chosen to determine genetic and genotypic diversity and to test the hypothesis of random mating and population differentiation of Rcc isolates. Among the 337 isolates collected, the overall gene diversity was moderate (ĥ = 0.216). The level of multilocus genotypic diversity was higher within populations than among them. All individuals had unique multilocus genotypes. Genetic differentiation was significant among populations in localities, but at a moderate level (h = 0.12; P < 0.001), suggesting that gene flow is occurring among populations. The isolates from all twelve clusters produced by Structure were found in all local populations, although at different frequencies. Therefore, the inferred clusters did not represent geographical populations. Although the null hypothesis of random mating in Rcc populations was rejected, the high level of genotypic diversity suggests that the Rcc population structure appears to be generated by a mixed reproductive system including both asexual and sexual reproduction, along with a rather high migration rate.

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Section: Discussionmentioning

confidence: 99%

“…The 406‐bp sequence of the Rcc cytochrome b gene was amplified by PCR (Matusinsky et al. ). The reactions were carried out in 20 μl volumes, containing 10 ng of fungal DNA.…”

Section: Methodsmentioning

confidence: 99%

Variability of the Ramularia collo‐cygni Population in Central Europe

Leišová‐Svobodová

Matušinsky

Kučera

2012

Journal of Phytopathology

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“…The first products to be registered were based on prothioconazole (demethylation-inhibitor: DMI) and chlorothalonil (Ctl, multi-site), followed by boscalid Table 2) has given barley growers a significant boost in their options to control pathogens (Russell, 2009) However there are concerns surrounding stewardship of SDHIs and asRcc is capable of relatively rapid resistance build up (Fountaine and Fraaije 2009;Matusinsky et al 2010) guidelines for their use have been produced (FRAC 2014).…”

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confidence: 99%

Ramularia collo-cygni—An Emerging Pathogen of Barley Crops

et al. 2015

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Ramularia collo-cygni is the biotic factor responsible for the disease Ramularia leaf spot (RLS) of barley (Hordeum vulgare). Despite having been described over 100 years ago and being considered a minor disease in some countries, the fungus is attracting interest in the scientific community as a result of the increasing number of recorded economically damaging disease epidemics. New reports of disease spread and fungal identification using molecular diagnostics have helped redefine RLS as a global disease. This review describes recent developments in our understanding of the biology and epidemiology of the fungus, outlines advances made in the field of the genetics of both the fungus and host, and summarizes the control strategies currently available.

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“…There were no significant differences in RLS control between the two application timings, although the splitting and late application increased RLS control marginally compared to an early application. Fungicide applications between GS 37 and GS 55 are considered optimal for disease control (Matusinsky et al 2010). McCabe (2009) also reported GS 49 or later as optimal fungicide timing for ensuring yield, late season fungicide applications are considered the most effective tool to protect leaves and ears from the fungus (Havis et al 2014;Heß et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Fungicide strategies and resistance of Ramularia collo-cygni to demethylation and succinate dehydrogenase inhibitors in Austrian winter barley (Hordeum vulgare)

Assinger¹,

Torriani

Accardo

et al. 2021

J Plant Dis Prot

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Ramularia collo-cygni B. Sutton and J.M. Waller is a major disease in Austrian barley-growing regions. To date, fungicide application is the most effective method to manage the disease; however, fungicide resistance to demethylation and succinate dehydrogenase inhibitors has developed over the last few years. In the growing seasons 2016/2017 and 2017/2018, field trials were carried out to analyze the efficiency of fungicide strategies based on different fungicide classes. Disease development, growth parameters and monitoring of CYP51 and sdh mutations were determined. Fungicide treatments resulted in higher disease control, green leaf area and grain yield. In Austrian R. collo-cygni field populations, the frequency of the mutations CYP51-I325T and CYP51-I328L was low to moderate. Frequency of mutations sdhC-H146R and sdhC-H153R was low. Frequencies of CYP51-I325T and -I328L were similar and increased following DMI application. Frequency of sdhC-H146R was higher compared to sdhC-H153R. The SDHI benzovindiflupyr showed a higher selection rate for sdh mutations compared to bixafen. These sdh mutations were not selected if chlorothalonil was used as mixing partner, leading to a stable composition of sdh resistance alleles over the last two years. Chlorothalonil was proven to be an effective tool for anti-resistance strategies. Currently, SDHIs and DMIs are the backbone of Ramularia leaf spot control in Austria; however, the level of resistance is likely to increase in absence of suitable anti-resistance strategies and following the ban of chlorothalonil.

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Frequency of a mutant allele of cytochrome b conferring resistance to Ool fungicides in the Czech population of Ramularia collo-cygni

Cited by 21 publications

References 10 publications

Variability of the Ramularia collo‐cygni Population in Central Europe

Variability of the Ramularia collo‐cygni Population in Central Europe

Ramularia collo-cygni—An Emerging Pathogen of Barley Crops

Fungicide strategies and resistance of Ramularia collo-cygni to demethylation and succinate dehydrogenase inhibitors in Austrian winter barley (Hordeum vulgare)

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