Frequency dependent and surface characterization of DNA immobilization and hybridization

Hang, Tin Christopher; Guiseppi‐Elie, Anthony

doi:10.1016/j.bios.2003.12.014

Cited by 70 publications

(29 citation statements)

References 62 publications

(54 reference statements)

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“…Laureyn et al characterized IDEs with nanoscale fingers and reported an impedance change during probe immobilization but did not report on target binding [213]. Hang et al described changes in impedance between Pt IDEs upon DNA hybridization [214]. Very large DNA concentrations were used.…”

Section: Interdigitated Electrodesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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Section: Interdigitated Electrodesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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“…[9][10][11][12][13][14][15][16] The catalytic behaviour of enzymes can be determined as a function of time and potential. [17] Certain adhering proteins [5] can be identified and the thickness of biofilm layers can be estimated.…”

Section: Application Of a Biomaterials Requires Profound Knowledge Abomentioning

confidence: 99%

“…[4] The impedance increases due to a change of the degree of freedom of translation and rotation, which occur due to adsorption reactions, network formation and a decrease of polarizability of functional groups. [11] Electrostatic van-der-Waals interactions exist between the molecules at the interface. The energy is a function of the distance, there are energy minima.…”

Section: Protein Adsorption At Biomaterialsmentioning

confidence: 99%

“…Diffusion behaviour of redox probes change with antibody adsorption. Hybridization reactions of ssDNA modified electrodes lead to an increase of the CTR [10,11,63] and the capacitance [63] due to covalent binding. Water is released during hybridization reactions and the impedance decreases due to a lower ADVANCED ENGINEERING MATERIALS 2008, 10, No.…”

Section: Electrochemical Impedance To Sense Antigens and Dnamentioning

confidence: 99%

“…10 density of water molecules and solvated ions. [11] The Nyquist plot of the stepwise formation of alternating DNA and PDDA multilayers depicts increasing semicircles. The charge transfer rate of the redox probe Fe(CN) 6 4-/3-decreases due to an insulating effect of the formed layers.…”

Section: Electrochemical Impedance To Sense Antigens and Dnamentioning

confidence: 99%

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Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

2008

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Electrochemical impedance spectroscopyElectrochemical impedance spectroscopy is an easy applicable sensitive technique. It is used to get information about the adsorption behaviour and the interactions of biomolecules with the electrode surface in a short measurement duration. Electrochemical processes can be modelled by an equivalent circuit and various parameters reflecting certain properties of the interface can be determined. The electrochemical properties of the interface are influenced by proteins and cells adsorbing at the surface. Different modified surfaces can be analyzed before, during, and after cell contact, respectively. Applying bias potentials also changes the behaviour of attaching cells and proteins. Changes in the medium composition have an influence on the electrochemically detectable surface properties.Biosensors are developed consecutively to detect biomolecules. Enzymes, lipids and membranes can be analyzed and the adsorption behaviour of DNA and antigens at modified electrode surfaces can be determined. Different technologies using microchips were constructed to allow a fast screening of electrochemical properties and discrimination of biological molecules or cells. ApplicationElectrochemical impedance spectroscopy serves not only for determination of the capacity of new battery materials or corrosion behaviour and long term stability of diverse alloys and oxide layers, it can also be used to gain information about biomolecules. The adsorption behaviour of cells [1][2][3] and proteins, [4] the amount of adsorbed proteins, [4][5][6][7] surface charge densities [4][5][6] and adsorption coefficients [5] can be determined. Interactions of implant surfaces with biological devices, changes in the polarizability of functional groups, the transport of ions or dipoles through polymer layers and mineralization processes, for instance of bone tissue can be assessed. The change of the Gibbs free energy and the entropy due to protein binding to the surface can be calculated [4,6,8] and the affinity of biomolecules to the surface can be estimated. [4] Electrochemical techniques are introduced as biosensors. Antigens, ssDNA or enzymes are immobilized at the surface and according antibodies, DNA and distinct proteins can be ADVANCED ENGINEERING MATERIALS 2008, 10, No. 10

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Electrical‐Impedance‐Based Sensors

Bănică

2012

Chemical Sensors and Biosensors

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Frequency dependent and surface characterization of DNA immobilization and hybridization

Cited by 70 publications

References 62 publications

Label‐Free Impedance Biosensors: Opportunities and Challenges

Label‐Free Impedance Biosensors: Opportunities and Challenges

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

Electrical‐Impedance‐Based Sensors

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