Frequency and susceptibility pattern of uropathogenic Enterobacteriaceae isolated from patients in Algiers, Algeria

Abstract

Introduction: The frequency of Enterobacteriaceae involved in urinary tract infections (UTI) has increased significantly since the early 1990s, particularly in at-risk facilities such as resuscitation, surgery, urology and nephrology. The objective of this study was to evaluate the antimicrobial susceptibility of Enterobacteriaceae causing urinary tract infections (UTIs)at the University Hospital Centre of Benimessous in Algiers.
 Methodology: The study was designed as a retrospective study (between January 1st 2010 and December 31st 2012) and a prospective study (between January 1standApril 30th 2013) on 13,611 urine samples. Antimicrobial resistance phenotyping was conducted on the bacterial isolates using disk-diffusion method.
 Results: On 13,611 urine samples analysed, 1,790 (13.15%) fulfilled the criteria for urinary tract infection. Enterobacteriaceae were identified in 1,561 analysed samples (87%). Escherichia coli was the dominant uropathogen (66,15%) in both hospitalized and non-hospitalized patients. The other main detected Enterobacteriaceae members were Klebsiella pneumoniae (11,96%) and Proteus mirabilis (5,42%). Analysis of results showed also that women were more prone to UTI than men with sex ratio of 3.76(W/M).
 The susceptibilities of isolated Enterobacteriaceae to antibiotics revealed that they had acquired resistance to several classes, particularly toward β-lactams. Resistance frequencies were relatively high to ampicillin and sulfomethoxasole, while being very low to aminoglycosides and furans. Results obtained revealed also that 7% of isolates where resistant to third generation cephalosporins by production of extended spectrum β-lactamases (ESBL).
 Conclusions: The continuous monitoring of antibiotic resistance of uropathogenic Escherichia coli is crucial to guide the clinician to choose the best empiric treatment.

Frequency and Antibiotic Resistance of Enterobacteriaceae Isolated in Community Urinary Tract Infections at Tebessa Region

“…The second commonest isolate was Klebsiella pneumoniae which accounted for 64 (33%) followed by Pseudomonas aeruginosa (6%), Proteus mirabilis (5%), Acinetobacter bumannii (4%) and Providencia stuartii (1%) (Figure 1). These findings are in agreement with results found in Constantine hospital (Eastern Algeria) [7] and those reported at the University Hospital Centre of Benimessous in Algiers [8]. In contrast to our results Thirtyeight Enterobacteriaceae isolates including seventeen Klebsiella pneumonia, thirteen Escherichia coli and eight Enterobacter cloacae were recovered from community-acquired urinary-tract infections at four private-laboratories in Bejaia, Algeria, between March 2007 and May 2009 [9].…”

Antibiotic Resistance Pattern of Enterobacteriaceae Strains Isolated from Community Urinary Tract Infections in Algiers, Algeria

“…There were 116 strains (87.22%) of Enterobacteriaceae (Figure 1), among which 76 (65.51%) were identified as Escherichia coli. These findings are in agreement with results found in some regions of Algeria [5,14] and other countries [15,16]. The second most common species were Klebsiella pneumoniae and Proteus mirabilis, each of which was represented by 13 strains (11.20%).…”

“…UTIs are on the rise globally and estimated to account for around 8.6 million healthcare visits annually [2]. Moreover, antibiotic resistance has risen to dangerously high levels globally, and by 2050, it is estimated that 10 million deaths will be due to antimicrobial resistance and that $100 trillion of the global economy will be lost if consistent efforts are not made to contain this threat [4,5]. In Algeria, the incidence of UTIs has increased significantly since the early 1990s, especially in vulnerable healthcare settings such as intensive care units and surgeries [5].…”

“…Moreover, antibiotic resistance has risen to dangerously high levels globally, and by 2050, it is estimated that 10 million deaths will be due to antimicrobial resistance and that $100 trillion of the global economy will be lost if consistent efforts are not made to contain this threat [4,5]. In Algeria, the incidence of UTIs has increased significantly since the early 1990s, especially in vulnerable healthcare settings such as intensive care units and surgeries [5]. Furthermore, several published works reported the alarming appearance of new multi-resistant bacterial strains in Algeria [6].…”