Frequency and structure of macrophages and abnormal sperm cells in guinea fowl semen

Hess, Rex A.; Hughes, B. L.; Thurston, R. J.

doi:10.1051/rnd:19860104

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…This makes it difficult to improve the genetic quality of farmed poultry efficiently, in which birds subjected to intense breeding conditions have exhibited a rapid decrease in genetic diversity 9. Several studies have detailed the morphological features of both normal and defective spermatozoa in the chicken1011 and Guinea fowl;121314 however, the basic reproductive biology related to sperm morphometry has received little attention.…”

Section: Introductionmentioning

confidence: 99%

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

García-Herreros

2016

Asian J Androl

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The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris) as model avian species. Seventy-two ejaculates (36 per species studied) were obtained manually, following a training period involving gently combined dorso-abdominal and lumbo-sacral massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry (CASA-Morph) methods, with sperm morphometric descriptors evaluated by Principal Component Analysis (PCA) and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration (P < 0.001). Irrespective of species, PCA revealed two Principal Components (PCs) explaining more than 80% of the variance. In addition, the number of subpopulations differed with species (three and five subpopulations for rooster and Guinea fowl, respectively). Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

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Section: Introductionmentioning

confidence: 99%

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

García-Herreros

2016

Asian J Androl

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“…In avian species, spermiophages have been identified in the semen of guinea fowl (Hess et al. ) or in the rete testis of normal cockerels (Aire and Malmqvist ). Aire () reported active spermiophagy by the non‐ciliated cells in the epithelial lining of the epididymal efferent ducts of the normal chicken.…”

Section: Introductionmentioning

confidence: 99%

“…Transmission electron microscope was used to identify semen macrophages of guinea fowl (Hess et al. ), turkey (Thurston et al. ) or man (Pelliccione et al.…”

Section: Introductionmentioning

confidence: 99%

Influence of Macrophages on the Rooster Spermatozoa Quality

Kuželová

Vašíček

Chrenek

2015

Reprod Domestic Animals

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The goal of this study was to evaluate the occurrence of macrophages in rooster semen and to investigate their impact on the spermatozoa quality. Ross 308 breeder males (n = 30) with no evidence of genital tract infections were used to determine the concentration of macrophages using fluorescently conjugated acetylated low-density lipoprotein (AcLDL). Subsequently, the roosters were divided into two groups on the basis of semen macrophage concentration, and semen quality was compared in two heterospermic samples. We applied computer-assisted semen analysis (CASA) system to determine motility parameters. Fluorescence microscopy and flow cytometry were used to evaluate occurrence of apoptotic and dead spermatozoa. Spermatozoa fertility potential was examined after intravaginal artificial insemination of hens. Eighteen roosters (control group) contained 0.2-3% of macrophages within spermatozoa population and ten roosters (macrophage group) had 10-15% of macrophages. Males from macrophage group had lower (p < 0.05) motility parameters (total and progressive movement, velocity curved line) and increased concentration of dead spermatozoa detected by flow cytometry and fluorescence microscopy (p < 0.001 and p ˂ 0.05, respectively). Differences (p < 0.05) between fluorescent microscopy and flow cytometry in results on spermatozoa apoptosis and viability were observed. No significant difference was found between groups in fertility of spermatozoa. In conclusion, the higher presence of macrophages in rooster semen may have a negative effect on some parameters of rooster spermatozoa evaluated in vitro. Furthermore, our study suggests that flow cytometry allows more precise examination of spermatozoa viability and apoptosis in a very short time compared with the fluorescent microscopy.

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“…Although some ultrastructural descriptions of abnormal avian sperm have been provided by scanning [1][2][3][4] and transmission electron microscopy [2,[5][6][7], most information on this topic stems from light microscopic observations. The paucity of corroborative ultrastructural data and the resultant lack of critical appraisal of avian sperm defects have led to the use of disparate and often confusing terminology for their classification.…”

Section: Introductionmentioning

confidence: 99%

“…Some authors have adopted a generalized approach and simply refer to head, midpiece and tail (flagellum) bends [3,7,8], while others qualify the degree of bending, recognizing 90° and 180°h ead and tail bends as well as midpiece bending [9][10][11]. Other descriptions of bending are more specific and define the precise area where the bend occurs.…”

Section: Introductionmentioning

confidence: 99%

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

Plessis

Soley

2011

Theriogenology

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The accurate assessment of avian sperm abnormalities is hampered by a lack of descriptive data and by the confusing terminology currently in use. Critical appraisal of semen samples from the distal ductus deferens of the emu revealed that two closely related yet separate (distinct) defects previously collectively referred to as "bent sperm" or "crooked-necked sperm" could be identified by light and electron microscopy. Head-base bending typically involved a 180° bend at the base of the nucleus which placed the head and midpiece into close apposition and parallel to each other. No part of the neck or midpiece was involved and bending was restricted exclusively to the base of the nucleus. Incomplete chromatin condensation was always associated with the bend. Disjointed sperm, which superficially resembled "bent" sperm, showed complete separation of the neck from 2 the head-base at the level of the connecting piece. All structural elements of the neck region appeared normal. In both defects the region of contact between the head (nucleus) and the neck/midpiece was enclosed as a unit by the plasmalemma. Both defects were observed to originate in the testis; however, their subsequent expression in the ductus deferens cannot be ruled out. These results confirm that head-base bending of emu sperm represents a head defect, whereas disjointed sperm should be classified as a tail (neck/midpiece) defect.

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Frequency and structure of macrophages and abnormal sperm cells in guinea fowl semen

Abstract: Introduction.

Cited by 13 publications

References 20 publications

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

Influence of Macrophages on the Rooster Spermatozoa Quality

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

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