Frequency and determinants of Hepatitis B and C virusin general population of Farash Town, Islamabad

Asad, Muhammad; Ahmed, Farah; Zafar, Humaira; Farman, Sabir

doi:10.12669/pjms.316.7047

Cited by 21 publications

(12 citation statements)

References 12 publications

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“…[ 16 ] Another risk factor that was identified in our study was the history of surgery which is also in agreement with other studies conducted in Pakistan. [ 17 ] Family history with HCV is one of the significant factors on our side which is similar to another study which concludes that there are 2.5 times greater chances of being positive if you have a positive family history as compared to the general population. [ 18 ] Considering this, it is recommended that active screening of the families of HCV infected patients be done routinely.…”

Section: Discussionsupporting

confidence: 74%

Predictive factors for acquiring HCV infection in the population residing in high endemic, resource-limited settings

Khan

Shah

Ashraf

2021

J Family Med Prim Care

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Background: In a country like Pakistan, a high prevalence of HCV persists due to a lack of awareness among the masses and the absence of adequate medical facilities in less privileged areas. Therefore, this study aimed to develop a risk-based screening tool based on the identification of predictive factors for HCV in the adult population in Karachi, Pakistan, which can later be validated for implementation. Methodology: A case-control study design was adopted and data was collected through an interview-based questionnaire from among 284 patients visiting the Family Medicine Department at The Indus Hospital, Karachi on whom the anti-HCV antibody test was conducted. Received data was then entered and analyzed using Statistical Package for the Social Sciences (SPSS) version 21.0. Result: Analysis indicated that in the entire cohort, marital status, employment status, history of being operated on in the past, family history of HCV infection, and body piercing were the factors significantly associated with positive HCV antibody. Results thus achieved show that the anti-HCV-positive rate was higher in ever married, employed, having had surgery, and family history of HCV infection (aOR: 2.42, 3.5, 2.04 and 2.5, P = 0.043, 0.002, 0.011 and 0.005, respectively). Conclusions: It is concluded that future research may be conducted enrolling the heterogeneous population to further probe the HCV burden and incidence in our society to initiate educational purposes. This goal can be achieved through commercial advertisements and free public lectures for disease prevention and better health awareness among the masses and the curers.

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Section: Discussionsupporting

confidence: 74%

Predictive factors for acquiring HCV infection in the population residing in high endemic, resource-limited settings

Khan

Shah

Ashraf

2021

J Family Med Prim Care

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“…Several studies and investigators reported a lower prevalence of HBV infections than our observation. In a previous study, it was reported that the prevalence of HBsAg seropositivity among a population of 345 tested cases was relatively lower [13]. In the current study, blood transfusion was considered as an important were higher among males (62.26%) when compared with females (37.74%).…”

Section: Discussionmentioning

confidence: 43%

Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq

Abdulla

Hama

2020

UHD J SCI TECH

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Hepatitis B virus infection is caused by the hepatitis B virus, a major global health problem. This infection can lead to chronic conditions, followed by cirrhosis and hepatocellular carcinoma (HCC). The current study was aimed to detect HBV using serological and molecular techniques. During 2019, 300 blood samples were collected from Kurdistan Center for Hepatology and Gastroenterology in Sulaimani city. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) techniques were used for the detection of HBsAg and HBV DNA, respectively. Obtained results were revealed that 92 out of 300 tested patients (30.66%) seropositive for HBsAg. Among 92 seropositive patients, 53 were shown positive results for HBV DNA by RT-PCR. Dental clinic visiting and dialysis were among the important risk factors for HBV transmission. The vast majority of positive results were among males. Smokers showed relatively high rates of positive results. One-third of the referred patients who had liver complaints were positive for HBsAg. More than half of the seropositive patients showed RT-PCR positive results. It was concluded that the molecular method (RT-PCR) is more sensitive and gives a more accurate result than serology (ELISA). Therefore, it can be used as a diagnostic tool for HBV detection.

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“…HIV, HBV and HCV infections are a serious health burden in Pakistan. [11][12][13][14] Diagnosis and point of care strategies require thorough understanding of infection's severity in the Pakistani population. Elevated CF mt-DNA levels have been observed in different pathophysiological conditions.…”

Section: Discussionmentioning

confidence: 99%

Assessment of cell free mitochondrial DNA as a biomarker of disease severity in different viral infections

Ali

Waseem²,

Anis³

et al. 2020

Pak J Med Sci

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Objective: Cell Free mitochondrial DNA (CF mt-DNA) has emerged as a novel biomarker to investigate disease pathophysiology of different infections. The present study was designed to elucidate the association between CF mt-DNA, IL-6 and viral load in HIV, HBV and HCV infections and predict its role as a potential biomarker to assess the disease severity in viral infections. Methods: Total 120 blood samples were collected from January 2018 to December 2018 of HIV, HBV and HCV patients and healthy controls (30 samples in each group). DNA and RNA were extracted from the serum to determine the levels of CF mt-DNA and viral load, respectively. IL-6 from the serum of infected individuals was quantified with ELISA. Results: HCV patients showed the highest levels of CF mt-DNA, IL-6 and viral load, followed by HBV and HIV. Significant correlation was found between CF mt-DNA and IL-6 among the HBV patients (p=0.017). However, no significant correlation of CF mt-DNA was observed with IL-6 in HIV and HCV or with the viral load in any of the three infections. Conclusion: Elevated CF mt-DNA indicates its role in severity of viral infections. Independence of CF mt-DNA expression from viral load and IL-6 in case of HIV and HCV suggests involvement of other inflammatory pathways regulating CF mt-DNA elevation. doi: https://doi.org/10.12669/pjms.36.5.2476 How to cite this:Ali Z, Waseem S, Anis RA, Anees M. Assessment of cell free mitochondrial DNA as a biomarker of disease severity in different viral infections. Pak J Med Sci. 2020;36(5):---------. doi: https://doi.org/10.12669/pjms.36.5.2476 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Frequency and determinants of Hepatitis B and C virusin general population of Farash Town, Islamabad

Cited by 21 publications

References 12 publications

Predictive factors for acquiring HCV infection in the population residing in high endemic, resource-limited settings

Predictive factors for acquiring HCV infection in the population residing in high endemic, resource-limited settings

Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq

Assessment of cell free mitochondrial DNA as a biomarker of disease severity in different viral infections

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