Frequency and clinical impact of preoperative circulating tumor cells in resectable non-metastatic lung adenocarcinomas

Dandachi, Nadia; Tiran, Verena; Lindenmann, Joerg; Brčić, Luka; Fink-Neuboeck, Nicole; Kashofer, Karl; Absenger, Gudrun; Bezan, Angelika; Côté, Richard J.; Datar, Ram H.; Balić, Marija

doi:10.1016/j.lungcan.2017.10.003

Cited by 12 publications

(18 citation statements)

References 24 publications

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“…We did not identify any clinicopathological factors associated with the number of CTCs other than smoking index. However, our results on the association between CTC counts and smoking history are inconsistent with the data presented by Dandachi et al (6). We speculate that our prospectively recruited series was adenocarcinoma-dominant (84% of all cases), which would confound any correlation with the smoking index.…”

Section: Discussioncontrasting

confidence: 99%

“…Although CTC counts at each clinical stage did not show any significant differences, our data show that CTCs were detected in patients with non-metastatic lung cancer, which indicates the possible utility of this device in early stage lung cancer. CTC studies targeting non-metastatic lung cancer are limited and the CTC detection rate (positive rate) varies (6,7,(11)(12)(13). As we did not confirm whether the cells captured on our device are cancer cells using other methods such as morphological examination under white light in all cases, we did not define the number of CTCs as positive in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Blood has been targeted as a sample for capturing circulating tumor cells (CTC) or DNA for molecular testing in a less invasive manner (5). Moreover, some investigators reported that CTCs are detectable in patients with non-metastatic lung cancer, and that the detected CTCs correlate with clinical outcome (6,7). However, it is unknown whether CTCs detected in patients with metastatic and non-metastatic disease have the same biological characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Detection of circulating tumor cells in patients with lung cancer using metallic micro‑cavity array filter: A pilot study

et al. 2020

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We have developed a metallic micro-cavity array filter and an automated detection system for capturing circulating tumor cells (CTCs). In this single institutional pilot study, we assessed the ability of this device to detect CTCs in patients with lung cancer at each stage. Patients diagnosed with lung cancer, undergoing planned surgery for lung cancer, or suspected of having lung cancer were recruited (40 recruited and 2 excluded). Blood samples were obtained from the patients and 3 ml whole blood was applied to the device without any preparation. The captured cells were stained to differentiate the nucleus, and determine cytokeratin and CD45 expression. Subsequently, two operators blinded to clinical information counted the number of CTCs. Sample collection was performed at the time of recruitment, before treatment and ~3 months after initial blood collection. CTC counts at recruitment were 1.4±0.4, 1.8±1.2, 1.3±0.6 and 7.4±5.1 (mean ± SE) in clinical stages I, II, III and IV, respectively. No significant difference was observed among the stages. These data indicated the ability of this device to detect CTCs at early or non-metastatic stages of lung cancer. Further research on a larger scale is needed for a more accurate assessment of the device, and research on the utility of captured cells remains a future challenge.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of circulating tumor cells in patients with lung cancer using metallic micro‑cavity array filter: A pilot study

et al. 2020

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“…That epithelial cells are misidentified as CTCs by CellSearch when measured in the PV during surgery would explain previous findings summarized in Table 3. The CTC presence during or shortly after surgery in the peripheral circulation is strongly associated with disease recurrence and recurrence-free survival (7,9,10,(24)(25)(26)(27)(28). Suspected CTCs identified in the PV were also shown to be associated with survival, but the association is less well defined (16,(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…CTCs have been shown an independent prognostic marker of survival for patients with non-small cell lung cancer (NSCLC) (1)(2)(3)(4)(5)(6). In early stage NSCLC, the presence of CTCs detected in the peripheral blood after surgery has been associated with a shorter time to recurrence (7)(8)(9)(10)(11). Of note, in advanced disease, CTCs are only observed in around 30% of patients with NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Released Circulating Tumor Cells During Surgery for Non-Small Cell Lung Cancer

Tamminga

Wit

Wauwer

et al. 2020

Clinical Cancer Research

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Tumor cells from patients with lung cancer are expelled from the primary tumor into the blood, but difficult to detect in the peripheral circulation. We studied the release of circulating tumor cells (CTCs) during surgery to test the hypothesis that CTC counts are influenced by hemodynamic changes (caused by surgical approach) and manipulation.Experimental Design: Patients undergoing video-assisted thoracic surgery (VATS) or open surgery for (suspected) primary lung cancer were included. Blood samples were taken before surgery (T0) from the radial artery (RA), from both the RA and pulmonary vein (PV) when the PV was located (T1) and when either the pulmonary artery (T2 open) or the PV (T2 VATS) was dissected. The CTCs were enumerated using the CellSearch system. Single-cell whole-genome sequencing was performed on isolated CTCs for aneuploidy.Results: CTCs were detected in 58 of 138 samples (42%) of 31 patients. CTCs were more often detected in the PV (70%) compared with the RA (22%, P < 0.01) and in higher counts (P < 0.01). After surgery, the RA but not the PV showed less often CTCs (P ¼ 0.02). Type of surgery did not influence CTC release. Only six of 496 isolated CTCs showed aneuploidy, despite matched primary tumor tissue being aneuploid. Euploid so-called CTCs had a different morphology than aneuploid.Conclusions: CTCs defined by CellSearch were identified more often and in higher numbers in the PV compared with the RA, suggesting central clearance. The majority of cells in the PV were normal epithelial cells and outnumbered CTCs. Release of CTCs was not influenced by surgical approach.

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Neue Diagnostik für das Lungenkarzinom

et al. 2018

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Frequency and clinical impact of preoperative circulating tumor cells in resectable non-metastatic lung adenocarcinomas

Cited by 12 publications

References 24 publications

Detection of circulating tumor cells in patients with lung cancer using metallic micro‑cavity array filter: A pilot study

Detection of circulating tumor cells in patients with lung cancer using metallic micro‑cavity array filter: A pilot study

Analysis of Released Circulating Tumor Cells During Surgery for Non-Small Cell Lung Cancer

Neue Diagnostik für das Lungenkarzinom

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