Frequency and Clinical Association of Panton-Valentine Leukocidin-Positive &lt;b&gt;&lt;i&gt;Staphylococcus aureus&lt;/i&gt;&lt;/b&gt; Isolates: A Study from Kuwait

Al-Haddad, A; Udo, Edet E.; Mathew, Bindu; Dhar, Rita

doi:10.1159/000343906

Cited by 15 publications

(17 citation statements)

References 28 publications

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“…The study also revealed that 44.3% of the isolates carried genes for PVL. This was higher than the 14.6% PVL gene-positive MRSA reported recently in a Kuwait hospital 28 but lower than the 54.2% positive rate obtained in a Saudi Arabian hospital 15 , indicating the diversity of MRSA bearing PVL genes in the GCC countries. PVL gene-positive S. aureus have been associated with necrotic skin lesions and community-acquired necrotic pneumonia 24 .…”

Section: Discussioncontrasting

confidence: 72%

Genotyping of methicillin-resistant Staphylococcus aureus in the Sultan Qaboos University Hospital, Oman reveals the dominance of Panton–Valentine leucocidin-negative ST6-IV/t304 clone

Udo

Al-Lawati

Al-Muharmi

et al. 2014

New Microbes and New Infections

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The objective of this study was to determine the prevalence and distribution of methicillin-resistant Staphylococcus aureus (MRSA) genotypes circulating at a tertiary hospital in the Sultanate of Oman. A total of 79 MRSA isolates were obtained from different clinical samples and investigated using antibiogram, pulsed-field gel electrophoresis (PFGE), staphylococcal chromosome cassette mec (SCCmec), Spa typing and multilocus sequence typing (MLST). The isolates were susceptible to linezolid, vancomycin, teicoplanin, tigecycline and mupirocin but were resistant to tetracycline (30.4%), erythromycin (26.6%), clindamycin (24.1%), trimethoprim (19.0%), ciprofloxacin (17.7%), fusidic acid (15.2%) and gentamicin (12.7%). Molecular typing revealed 19 PFGE patterns, 26 Spa types and 21 sequence types. SCCmec-IV (86.0%) was the dominant SCCmec type, followed by SCCmec-V (10.1%). SCCmec-III (2.5%) and SCCmec-II (1.3%) were less common. ST6-IV/t304 (n = 30) and ST1295-IV/t690 (n = 12) were the dominant genotypes followed by ST772-V/t657 (n = 5), ST30-IV/t019/t021 (n = 5), ST22-IV/t852 (n = 4), ST80-IV/t044 (n = 3) and 18 single genotypes that were isolated sporadically. On the basis of SCCmec typing and MLST, 91.2% of the isolates were classified as community-associated MRSA and 8.8% of the isolates (consisting of four ST22-IV/t852, one ST239-III/t632, one ST5-III/t311 and one ST5-II/t003) were classified as healthcare-associated MRSA. The study has revealed the dominance of a Panton–Valentine leucocidin-negative ST6-IV/t304 clone and provided insights into the distribution of antibiotic resistance in MRSA at the tertiary hospital in Oman. It also highlights the importance of surveillance in detecting the emergence of new MRSA clones in a healthcare facility.

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Section: Discussioncontrasting

confidence: 72%

Genotyping of methicillin-resistant Staphylococcus aureus in the Sultan Qaboos University Hospital, Oman reveals the dominance of Panton–Valentine leucocidin-negative ST6-IV/t304 clone

Udo

Al-Lawati

Al-Muharmi

et al. 2014

New Microbes and New Infections

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“…According to AlFouzan et al. [ 25 ], out of 291 S. aureus isolates, 30.6% were MRSA. Genes for PVL were detected in 14.6% and 12.0% of the MRSA and MSSA isolates, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Both MRSA types carried SCCmec type III, IV, IVc and V genetic elements. [ 25 ] In another meta-analysis including 76 studies from 31 countries, PVL strains were strongly associated with skin and soft-tissue infections, while comparatively rarely with pneumonia, musculoskeletal infections, bacteremias and colonizing strains. [ 26 ]…”

Section: Resultsmentioning

confidence: 99%

Panton-Valentine leukocidin in community and hospital-acquiredStaphylococcus aureusstrains

Dal

Yanık

Özcan

et al. 2014

Biotechnology & Biotechnological Equipment

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Staphylococcus aureus causes serious hospital-acquired (HA) and community-acquired (CA) infections. Skin and soft-tissue infections especially are sometimes caused by strains harbouring Panton-Valentine leukocidin (PVL). PVL belongs to a family of bi-component leukocidal toxins produced by staphylococci. It is a pore-forming toxin encoded by lukF-PV and lukS-PV. A total of 70 S. aureus strains: 38 (54%) methicillin-resistant (MRSA) and 32 (46%) methicillin-susceptible (MSSA), were isolated from patients admitted to Dicle University Hospital (Turkey). Identification of S. aureus and antibiotics-susceptibility testing were performed with PHOENIX 100. PVL genes and mecA genes were detected by polymerase chain reaction. Of the 70 studied strains, 36 ones (51%) were community acquired and 34 ones (49%) were hospital acquired . A total of 38 (54%) strains were positive for mecA (mecA +), of which 32 ones (84%) were HA. Of the mecA − strains, 30 (94%) were CA. Of the 70 studied strains, 12 (17%) strains were PVL+: 8 (22%) of the 36 CA strains and 4 (12%) of the 34 HA strains. Of the 12 PVL+ strains, 4 strains were mecA +. The PVL positivity rate was 25% in MSSA, whereas 10.5% in MRSA. Of the overall PVL+ strains, seven strains were obtained from wounds; four ones from skin abscess; and one from blood culture. Taken together, the obtained results showed a substantial level of PVL genes in the studied region. Although PVL is known as a common virulence factor of CA MRSA, HA MRSA isolates in our study showed a considerable rate of PVL positivity.

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“…Many CA-MRSA strains carry PVL genes, whereas their frequency in MSSA and dominant HA-MRSA clones is much lower [15]. However, a few studies on PVL-positive MSSA have been published [154][155][156][157].…”

Section: Paper IImentioning

confidence: 99%

Epidemiological and molecular biological studies of multi-resistant methicillin-susceptible Staphylococcus aureus

Lindqvist¹

2014

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Antibiotic resistance is increasingly recognised as a major problem and threat. During the last decades Gram-positive bacteria in general, and methicillin-resistant Staphylococcus aureus (MRSA) in particular, have been in focus both concerning matters of antibiotic resistance and as pathogens causing health care-associated (nosocomial) infections. In contrast to MRSA, studies on clonal distribution of methicillin-susceptible S. aureus (MSSA) are scarce.However, interest in MSSA has increased since it was shown that MRSA emerges from susceptible backgrounds by acquisition of a staphylococcal cassette chromosome element, carrying the mecA gene encoding methicillin-resistance (SCCmec).In an outbreak investigation of MRSA in Östergötland County, Sweden, in 2005, a high incidence of MSSA isolates with concomitant resistance to erythromycin, clindamycin and tobramycin (ECT-R) was detected. Analysis showed that 91 % of the investigated isolates were genetically related (clonal). The ECT-R clone was divided into four different but closely related patterns with pulsed-field gel electrophoresis (PFGE), and was designated spa type t002. Whole genome sequencing revealed that the ECT-R clone carried a pseudo-SCC element estimated to be 12 kb in size, showing a resemblance of more than 99 % with the SCCmec type II element of MRSA strain N315 (New York/Japan clone). This suggested a probable derivation from a highly successful MRSA strain, which had partially excised its SCCmec. The clonal outbreak was concentrated in eight hospital departments and two primary care centres, all located in the city of Linköping. Despite a high exchange of patients with the hospitals in the neighbouring counties in southeast Sweden (Jönköping-and Kalmar County), the ECT-R clone seemed to be limited to Östergötland County. However, a tobramycin-resistant clone predominated by isolates of spa type t084 was found in all three counties in southeast Sweden, and in particular among newborns, suggesting inter-hospital transmission.The ECT-R clone has survived as an abundant MSSA clone for a decade in Östergötland County, which indicates an insufficiency in the maintenance of basic hygiene guidelines, and that the clone probably possesses mechanisms of virulence and transmission that are yet to be discovered. Sammanfattning på svenska

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Frequency and Clinical Association of Panton-Valentine Leukocidin-Positive <b><i>Staphylococcus aureus</i></b> Isolates: A Study from Kuwait

Cited by 15 publications

References 28 publications

Genotyping of methicillin-resistant Staphylococcus aureus in the Sultan Qaboos University Hospital, Oman reveals the dominance of Panton–Valentine leucocidin-negative ST6-IV/t304 clone

Genotyping of methicillin-resistant Staphylococcus aureus in the Sultan Qaboos University Hospital, Oman reveals the dominance of Panton–Valentine leucocidin-negative ST6-IV/t304 clone

Panton-Valentine leukocidin in community and hospital-acquiredStaphylococcus aureusstrains

Epidemiological and molecular biological studies of multi-resistant methicillin-susceptible Staphylococcus aureus

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