Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra

Maluta, Renato Pariz; Borges, Clarissa Araújo; Beraldo, L. G.; Cardozo, Marita Vedovelli; Voorwald, Fabiana Azevedo; Santana, A. M.; Rigobelo, Everlon Cid; Toniollo, Gilson Hélio; Ávila, Fernando Antônio de

doi:10.1016/j.tvjl.2014.08.016

Cited by 12 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strains of these that are isolated from pyometra are those that normally inhabit canine intestine and those present in urinary tract infections. 28 Escherichia coli isolated from canine pyometra is mainly assigned to phylogenetic group B2 and characterized by a high number of uropathogenic E. coli (UPEC) virulence factor (VF) genes and pathogenicity-associated island markers. 28 Escherichia coli have VF genes encoding for adhesins (including fimH, papC and papGIII), toxins (including hlyA/F, usp and astA), and other factors (e.g.…”

Section: Escherichia Colimentioning

confidence: 99%

“…28 Escherichia coli isolated from canine pyometra is mainly assigned to phylogenetic group B2 and characterized by a high number of uropathogenic E. coli (UPEC) virulence factor (VF) genes and pathogenicity-associated island markers. 28 Escherichia coli have VF genes encoding for adhesins (including fimH, papC and papGIII), toxins (including hlyA/F, usp and astA), and other factors (e.g. those involved in escape from host defences and iron uptake).…”

Section: Escherichia Colimentioning

confidence: 99%

“…those involved in escape from host defences and iron uptake). 28 Frequency of VF genes in E. coli isolates from canine pyometra are summarized in Table 2. Escherichia coli isolates with > 2 adhesin genes imply that a combination of genes confers an advantage.…”

Section: Escherichia Colimentioning

confidence: 99%

“…Escherichia coli isolates with > 2 adhesin genes imply that a combination of genes confers an advantage. 28 Strongly virulent strains of E. coli (e.g. nau-b) contain 7 virulence genes.…”

Section: Escherichia Colimentioning

confidence: 99%

See 3 more Smart Citations

Reproductive microbiome alterations: canine pyometra

Kutzler

2019

View full text Add to dashboard Cite

Pyometra is a life-threatening uterine infection that affects 20 - 25% of reproductively intact bitches. Canine pyometra is characterized by accumulation of leukocytes and exudate within the uterine lumen. Although exact temporal and progressive mechanisms underlying its pathogenesis are not fully understood, a combination of endocrine, structural, inflammatory and bacterial factors are likely involved in most cases. Pyometra develops during luteal phase; progesterone has a key role in establishment of infection with opportunistic bacteria. Cystic endometrial hyperplasia results from proliferation of endometrial glands and is a predisposing factor for development of pyometra. Endometrial proliferation factors exacerbate uterine innate immune responses. Whereas Escherichia coli is the most common organism isolated in up to 90% of cases with a positive culture, genes encoding for adhesins, toxins, and other factors increase virulence of certain strains. A presumptive diagnosis of pyometra is based on clinical signs and laboratory tests, with a confirmatory diagnosis based on B-mode ultrasonography, with or without Doppler ultrasonography, detecting an enlarged, fluid-filled uterus with increased blood flow. Broad-spectrum antibiotics with minimal nephrotoxicity are needed to prevent septicemia, but antibiotics alone will not resolve pyometra. Safest and most effective treatment is surgical removal of infected uterus and ovaries. Medical management of pyometra may be indicated to improve general status of the bitch prior to surgery or treatment in valuable breeding bitches. Protocols using prostaglandin F2α or aglepristone are discussed in detail.

show abstract

Section: Escherichia Colimentioning

confidence: 99%

Section: Escherichia Colimentioning

confidence: 99%

Section: Escherichia Colimentioning

confidence: 99%

“…Escherichia coli isolates with > 2 adhesin genes imply that a combination of genes confers an advantage. 28 Strongly virulent strains of E. coli (e.g. nau-b) contain 7 virulence genes.…”

Section: Escherichia Colimentioning

confidence: 99%

See 2 more Smart Citations

Reproductive microbiome alterations: canine pyometra

Kutzler

2019

View full text Add to dashboard Cite

show abstract

“…In contrast, commensal intestinal strains of E. coli in dogs are mostly classified into phylogenetic groups B1 and A [ 5 , 9 , 12 ]. In addition, E. coli recovered from pyometra have specific virulence factors, such as adhesins, toxins, iron acquisition systems, and protectins [ 7 , 14 , 15 ], which are commonly classified as endometrial pathogenic E. coli (EnPEC), a subgroup of the ExPEC pathotype. These virulence factors may confer a selective advantage over commensal strains [ 16 ], playing a key role in the development of canine pyometra [ 11 , 14 , 17 ] as well as in other extraintestinal infections in humans and animals [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Escherichia coli in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic E. coli (ExPEC)

Xavier

Silva

Trindade

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

Despite its high frequency and clinical relevance, the pathogenesis of canine pyometra remains poorly understood. The most accepted hypothesis is that bacteria involved ascend from the intestinal tract, causing the uterine infection. Extraintestinal pathogenic Escherichia coli (ExPEC) is the most frequent pathogen in canine pyometra, accounting for 57–100% of cases. The aim of the present study was to determine the frequency of phylogenetic groups and virulence factors in E. coli strains isolated from the uterine and rectal swabs of bitches with pyometra (n = 72) and from rectal swabs from healthy bitches fed commercial dry feed (n = 53) or a raw meat-based diet (RMBD; n = 38). A total of 512 strains of E. coli were isolated and divided into five categories according to the origin of the sample: 120 isolates from the uterine content of dogs with E. coli pyometra, 102 from the feces of bitches with E. coli pyometra, 75 from the feces of bitches without E. coli pyometra, 130 feces samples from healthy dogs fed commercial feed, and 85 feces samples from healthy dogs fed a raw meat-based diet. E. coli strains belonging to the B2 phylogroup and positive for virulence factor genes associated with adhesion (fimbriae type P [papC]) and production of toxins (α-hemolysin [hlyA] and uropathogenic specific protein [usp]) predominated in the uterine content and rectal swabs of bitches with E. coli pyometra. Interestingly, a lower growth rate of E. coli from the B2 phylogroup was observed in dogs fed a RMBD than in those fed commercial dry feed. The present study suggests that intestinal colonization by certain types of E. coli could be a risk factor for the occurrence of E. coli pyometra in bitches and that diet can influence intestinal colonization by such strains.

show abstract

Escherichia coli

Fairbrother¹,

Gyles²

2022

Pathogenesis of Bacterial Infections in Animals

View full text Add to dashboard Cite

Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra

Cited by 12 publications

References 10 publications

Reproductive microbiome alterations: canine pyometra

Reproductive microbiome alterations: canine pyometra

Characterization of Escherichia coli in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic E. coli (ExPEC)

Escherichia coli

Contact Info

Product

Resources

About