Frequencies of the PRNP Gene Polymorphisms in Binglangjiang Buffalo (Bubalus bubalis) for Comparing Potential Susceptibility to BSE

Abstract

The bovine spongiform encephalopathy (BSE), is a neurodegenerative disorder initiated by miss folded prion protein affecting in cattle. It is associated with a 23 bp indel polymorphism in the putative promoter and a 12 bp indel in intron 1 of the PRNP gene. There is no prior record of investigating for these indels polymorphism from Binglangjiang (BLJ) buffalo. The results of collected 100 samples from BLJ buffalo demonstrated that high insertion of genotype frequencies in 23++ (0.98) and 12++ (0.93) with the allelic frequencies also high in 23 bp (0.980) and 12 bp (0.965) while deletion is considerably low or absent in the allelic as well as in genotype frequencies. Haplotypes data showed different indel polymorphism with absent deletion (0) in both regions of this PRNP gene. A comparative analysis of BLJ buffalo with healthy and BSE affected German and Swiss cattle breeds from European countries was done with the results signifying a difference (P<0.001) in healthy and affected cattle. Significant outcomes were observed after comparison with previous studies on BLJ buffalo. Result demonstrated no genetic vulnerability to in BJL buffalo. Thus, BLJ buffalo can therefore prove to be the most likely model for genetic, selection, breeding and production. To best of our knowledge, this was first study describing indels polymorphism 23 bp & 12 bp in BLJ buffalo (river buffalo) of China. Frequencies of the PRNP gene polymorphisms in Binglangjiang buffalo (Bubalus bubalis) for comparing potential susceptibility to BSE. Pak Vet J, 38(3): 256-260. http://dx.

Comparative Analysis of PRNP Gene Indel Polymorphism and Expression among Zhongdian Yellow Cattle, Zhongdian Yak, and Their Hybrids

“…BSE is a fatal neurodegenerative disorder caused by abnormal metabolism of prion protein (PrP) [30][31][32][33][34][35]. It has been reported that these cellular prion proteins (PrP C ) are encoded by prion proteins whose expressions show a vital role in prion disease susceptibility [36].…”

Compare analysis of the PRNP gene indels polymorphism and expression among Yellow cattle, Zhongdian Yak and their hybrid

“…BSE is a fatal neurodegenerative disorder caused by abnormal metabolism of prion protein (PrP) [31][32][33][34][35] . It has been reported that these cellular prion proteins (PrP C ) are encoded by prion protein whose expression shows a vital role in prion disease susceptibility 36 .…”