Frequencies of human neutrophil antigen-4 and human neutrophil antigen-5 among Thai blood donors

Abstract

Context:Antibodies against human neutrophil antigens (HNAs) are implicated in immune-mediated neutropenia, transfusion-related acute lung injury and febrile transfusion reactions.Aims:This study aimed to determine HNA gene frequencies of the HNA-4 and HNA-5 systems among Thai populations and compare these frequencies with those previously reported for other populations.Materials and Methods:800 DNA samples obtained from 500 unrelated healthy blood donors from Bangkok and 300 samples from Chiang Mai, Thailand were included. Samples were typed for each HNA allele including HNA-4a, HNA-4b, HNA-5a, and HNA-5b using an in-house polymerase chain reaction with sequence-specific primer technique.Results:The frequencies of HNA-4a and HNA-4b alleles in central Thais were 0.975 and 0.025, respectively and for Northern Thais, their frequencies were 0.965 and 0.035, respectively. For HNA-5a and HNA-5b alleles, their frequencies were 0.771 and 0.229; 0.748, and 0.252 in central and Northern Thais, respectively. The frequencies of HNA-4 and HNA-5 systems in central Thais are closely related to those in Northern Thais (P > 0.05). However, their frequencies were different from other populations (P < 0.001), except HNA-5a and HNA-5b gene frequencies in Thais were similar to Caucasians (P > 0.05).Conclusion:This study could contribute to predict the risk of alloimmunization to HNA-4 and HNA-5 systems, especially in feto-maternal incompatibility in Thais.

Integrin Subunit Alpha M, ITGAM Nonsynonymous SNP Is Associated with Knee Osteoarthritis among Thais: A Case-Control Study

“…Among the 117 symptomatic knee OA cases in this study, the prevalence of unilateral and bilateral KL ≥ 2 was 3.0 and 16.5%, respectively (Table 6), whereas a high prevalence was found of 12.5 and 34.1% in Beijing, and 15.2 and 19.7% in Framingham studies [38]. The high prevalence of bilateral knee OA may indicate that environmental or ethnic (genetic) factors are among the essential factors in the development of knee OA; however, we did not find an association between knee OA severity (by KL grade) and significant variables (female, ≥57 years old, and ITGAM*01*01 genotype) in this study.…”

“…Having the ITGAM*01*02 genotype could also mean a reduced risk for knee OA and delayed disease progression. However, the occurrence of the ITGAM*02*02 genotype is very rare in Thailand and other populations [17,18,38], thus the uninformative nature of this genotyping result cannot confirm or indicate whether an individual either has a reduced risk for or is protected from developing knee OA.…”