Freezing tolerance, protein composition, and abscisic acid localization and content of pea epicotyl, shoot, and root tissue in response to temperature and water stress

Welbaum, Gregory E.; Bian, Desong; Hill, D.R.; Grayson, R. L.; Gunatilaka, M.K.

doi:10.1093/jxb/48.3.643

Cited by 32 publications

(25 citation statements)

References 29 publications

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“…During freeze-thaw cycles, exogenous glycine betaine prevented changes in relative proportion and antenna sizes of PSII in pea (Busheva and Apostolova, 1997). Exogenous ABA treatment improved freezing tolerance, but did not replace cold hardening in peas (Welbaum et al, 1997).…”

Section: Physiological and Biochemical Testsmentioning

confidence: 90%

Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes

et al. 2006

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The adaptability and productivity of cool-season food legumes (chickpea, faba bean, lentil, pea) are limited by major abiotic stresses including drought, heat, frost, chilling, waterlogging, salinity and mineral toxicities. The severity of these stresses is unpredictable in field experiments, so field trials are increasingly supplemented with controlledenvironment testing and physiological screening. For drought testing, irrigation is used in dry fields and rain-out shelters in damp ones. Carbon isotope discrimination ( 13 C) is a well-established screen for drought tolerance in C3 cereal crops which is now being validated for use in grain legumes, but it is relatively expensive per sample and more economical methods include stomatal conductance and canopy temperature. Chickpea lines ICC4958 and FLIP87-59C and faba bean line ILB938 have demonstrated good drought tolerance parameters in different experiments. For frost tolerance, an efficient controlled-environment procedure involves exposing hardened pot-grown plants to subzero temperatures. Faba beans Cote d'Or and BPL4628 as well as lentil ILL5865 have demonstrated good freezing tolerance in such tests. Chilling-tolerance tests are more commonly conducted in the field and lentil line ILL1878 as well as derivatives of interspecific crosses between chickpea and its wild relatives have repeatedly shown good results. The timing of chilling is particularly important as temperatures which are not lethal to the plant can greatly disrupt fertilization of flowers. Salinity response can be determined using hydroponic methods with a sand or gravel substrate and rapid, efficient scoring is based on leaf symptoms. Many lines of chickpea, faba bean and lentil have shown good salinity tolerance in a single article but none has become a benchmark. Waterlogging tolerance can be evaluated using paired hydroponic systems, one oxygenated and the other de-oxygenated. The development of lysigenous cavities or aerenchyma in roots, common in warm-season legumes, is reported in pea and lentil but is not well established in chickpea or faba bean. Many stresses are associated with oxidative damage leading to changes in chlorophyll fluorescence, membrane stability and peroxidase levels. An additional factor relevant to the legumes is the response of the symbiotic nitrogen-fixing bacteria to the stress.

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Section: Physiological and Biochemical Testsmentioning

confidence: 90%

Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes

et al. 2006

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“…Endogenous ABA level increases under stress conditions, most notably under drought and salinity (Munns and Cramer 1996;Zdunek and Lips 2001;Yang and Guo 2007). Application of exogenous ABA has similar effects on plants as stress treatments and increases plant tolerance to environmental stresses (Fedina et al 1994;Welbaum et al 1997). Elucidation of the ABA biosynthetic pathway, as well as related genes and enzymes, is important for the control and regulation of ABA-mediated responses in plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

et al. 2010

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An aldehyde oxidase gene, designated as aldehyde oxidase2 (AhAO2), was cloned from Arachis hypogaea L. The gene contained a 4,050-bp open reading frame that encoded a putative protein of 1,350 amino acids. The deduced amino acid sequence showed high identity with other plant AOs. The organ-specific expression pattern of AhAO2 has been examined, which indicates its dominant expression in leaves of peanut. The AhAO2 transcript level in leaves was greatly increased under exogenous ABA treatment for 1 h. Over-expression of AhAO2 in Arabidopsis led to an increase in ABA level and enhanced drought tolerance after drought treatment. These results indicated the possible involvement of AhAO2 in ABA synthesis and drought tolerance.

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“…Glycine betaine plays an important role as a compatible solute in plants, which varies among species and accumulates at higher levels in the chloroplasts (Sakamoto and Murata 2002). Stoddard et al (2006) suggested the further analysis of its role as a screening marker for frost tolerance, whereas the treatment of ABA can improve the freezing tolerance but cannot induce cold hardening (Welbaum et al 1997). …”

Section: Discussionmentioning

confidence: 99%

“…Bourion et al (2003) found a relationship between sugar concentration in the leaves and freezing tolerance. An exo-glycine betaine-like proline prevents changes in antenna sizes of photosystem II (not explained before) in peas (Busheva and Apostolova 1997), and exo-ABA can increase the freezing tolerance (Welbaum et al 1997).…”

Section: Field Peamentioning

confidence: 95%

Radiant frost tolerance in pulse crops—a review

2009

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Radiant frost is a major abiotic stress, and one of the principal limiting factors for agricultural production worldwide, including Australia. Legumes, including field pea, faba bean, lentil and chickpea, are very sensitive to chilling and freezing temperatures, particularly at the flowering, early pod formation and seed filling stages. Radiant frost events occur when plants and soil absorb the sunlight during the day time and radiate heat during the night when the sky is clear and the air is still. Dense chilled air settles into the lowest areas of the canopy, where the most serious frost damage occurs. The cold air causes nucleation of the intracellular fluid in plant tissues and the subsequent rupturing of the plasma membrane. Among the cool season grain legume crops, chickpea, lentil and faba bean and field pea are the most susceptible to radiant frost injury during the reproductive stages. The more sensitive stages are flowering and podding. Frost at the reproductive stage results in flower abortion, poor pod set and impaired pod filling, leading to a drastic reduction in yield and quality. In contrast, in the UK and European countries, frost stress is related to the vegetative stages and, in particular, the effects of frost have been studied on cotyledon, uni/tri-foliolate leaf and seedling stages during the first few weeks of growth. Few winter genotypes have been identified as frost tolerant at vegetative stages. Vegetative frost tolerance is not related to reproductive frost tolerance, and hybrids from the vegetative frost-tolerant genotypes may not necessarily be tolerant at the reproductive stage. Tolerance to radiant frost has an inverse relationship with plant age. In the field, frost tolerance decreases from the vegetative stage to reproductive stage. Unlike wheat and barley, it is difficult to analyse and score frost damage in grain legume crops due to the presence of various phenophases on one plant at the reproductive stage. The extent of frost damage depends on the specific phenophases on a particular plant. However, current studies on genetic transformation of cold tolerant gene(s), membrane modifications, anti-freeze substances and ice nucleating or inhibiting agents provide useful information to improve our current understanding on frost damage and related mechanisms. The effects of frost damage on yield and grain quality illustrate the significance of this area of research. This review discusses the problem of A. Maqbool Pulses and Oilseeds, South radiant frost damage to cool season legumes in Australia and the associated research that has been carried out to combat this problem locally and worldwide. The available literature varies between species, specific climatic conditions and origin.

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Freezing tolerance, protein composition, and abscisic acid localization and content of pea epicotyl, shoot, and root tissue in response to temperature and water stress

Cited by 32 publications

References 29 publications

Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes

Screening techniques and sources of resistance to abiotic stresses in cool-season food legumes

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

Radiant frost tolerance in pulse crops—a review

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