Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution

Fuchinoue, Kohei; Kyono, Koichi; Fukunaga, Noritaka; Nakajo, Yukiko; Yagi, Akiko; Sasaki, Sachiko

doi:10.1016/s0015-0282(03)01271-8

Cited by 8 publications

(12 citation statements)

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“…Taxol has been used in cryopreservation of embryos (Dobrinsky et al 2000), and it has been found that Taxol pretreatment can improve developmental competence of vitrified mouse and human (Fuchinoue et al 2004) oocytes. However, to our knowledge, no study has reported the effect of Taxol treatment of porcine oocytes during vitrification.…”

Section: Introductionmentioning

confidence: 99%

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Shi¹,

Zhu

Zhang³

et al. 2006

Reproduction

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This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by an open pulled straw (OPS) method. In the first experiment, the effect of Taxol pretreatment and fluorescein diacetate (FDA) staining on parthenogenetic development of oocytes was evaluated. In the second experiment, viability, microtubule organization and embryo development of oocytes were assessed after oocytes were exposed to vitrification/warming solutions or after vitrification with or without Taxol pretreatment. The results showed that Taxol pretreatment and/or FDA staining did not negatively influence the oocyte's developmental competence after parthenogenetic activation. After being exposed to vitrification/warming solutions, the survival rate (83.3%) of the oocytes was significantly (P < 0.05) reduced as compared with that in the control (100%). Vitrification/warming procedures further reduced the survival rates of oocytes regardless of oocytes being treated with (62.1%) or without (53.8%) Taxol. The proportions of oocytes with normal spindle configuration were significantly reduced after the oocytes were exposed to vitrification/warming solutions (38.5%) or after vitrification with (10.3%) or without (4.1%) Taxol pretreatment as compared with that in control (76.8%). The rates of two-cell-stage (5.6-53.2%) embryos at 48 h and blastocysts (0 -3.8%) at 144 h after activation were significantly reduced after exposure to vitrification/warming solutions or after vitrification as compared with control (90.9% and 26.6% respectively). However, the proportion of vitrified oocytes developed to two-cell stage was significantly higher when oocytes were pretreated with (24.3%) than without (5.6%) Taxol. These results indicate that pretreatment of oocytes with Taxol before vitrification helps to reduce the damage induced by vitrification and is a potential way to improve the development of vitrified porcine oocytes. Reproduction (2006) 131 795-804

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Section: Introductionmentioning

confidence: 99%

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Shi¹,

Zhu

Zhang³

et al. 2006

Reproduction

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“…Caution must be used when interpreting the data presented in Table 2, since some of the studies included were of particularly small sample sizes. This was notably the case for all reports with the highest success rates: for slow-freezing, the 62% and 43% maturation and fertilization rates were obtained from a total of only 21 oocytes (Chen et al, 2004) and for vitrification, the 77%, 56%, and 47% maturation, fertilization, and cleavage rates were from a total of 30 oocytes (Fuchinoue et al, 2004). Also with vitrification, the rather high 12% blastocyst formation rate was obtained from a mere 17 starting oocytes (Chung et al, 2000).…”

Section: Percent Ranges Of Laboratory Outcomes With the Cryopreservatmentioning

confidence: 70%

“…In this way, the oocytes may be conditioned or prepared to resist cryoin-juries. A pilot study examined the effect of the drug taxol to help stabilize the microtubule cytoskeleton prior to cryopreservation (Fuchinoue et al, 2004). Results indicated some improvements, as previously suggested in animal models.…”

Section: Other Potential Opportunities For Improvementsmentioning

confidence: 80%

“…Unless noted otherwise, all studies discussed herein are in human. Table 2 summarizes the ranges of values obtained from a total of 23 studies that cryopreserved human oocytes at the GV stage, 14 and 12 of which used slow-freezing or vitrification protocols, respectively (Mandelbaum et al, 1988;Toth et al, 1994a;Toth et al, 1994b;Baka et al, 1995;Son et al, 1996;Park et al, 1997;Chung et al, 2000;Goud et al, 2000;Wu et al, 2001;Boiso et al, 2002;Chen et al, 2004;Fuchinoue et al, 2004;Isachenko et al, 2006;Cao et al, 2009;Fasano et al, 2010;Al-Khtib et al, 2011;Combelles et al, 2011;Criado et al, 2011;Liu et al, 2011;Versieren et al, 2011;Zhang et al, 2011;Fasano et al, 2012;Wang et al, 2012). Seventy percent (16/23) of studies evaluated the in vitro developmental competence of cryopreserved GV oocytes based on fertilization, cleavage, and in some instances even blastocyst formation.…”

Section: Advantages and Drawbacks Of Cryopreserving Immature Oocytes mentioning

confidence: 99%

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The use of immature oocytes in the fertility preservation of cancer patients: current promises and challenges

Combelles¹,

Chateau²

2012

Int. J. Dev. Biol.

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Improved oncological treatments permit increased survival rates, although cancer patients remain at risk of losing ovarian function. An attractive option for fertility preservation includes the use of immature oocytes, a strategy which can occur on a rapid timeline and without hormonal stimulation. As a result, cancer therapy can proceed promptly even in patients with hormone-sensitive tumors. Following retrieval, immature oocytes can be cryopreserved at either the immature germinal vesicle or the mature metaphase-II stage, i.e. either before or after in vitro maturation (IVM). We present a critical review of previous human studies on the cryopreservation of immature oocytes. Evaluations include in vitro developmental competence upon thawing/warming, or organization of the spindle and chromosomes. Reported successes vary, perhaps in relation to the source of the oocytes and protocols for cryopreservation and IVM. Weaknesses exist with the experimental designs implemented to date, so caution must be exercised before considering the use of immature oocytes to be a safe and reliable practice in the fertility preservation of cancer patients. To date, results indicate that with current protocols, it may be best to cryopreserve immature oocytes after IVM at the metaphase-II stage. Nonetheless, efficacy remains very low. Future efforts should tailor and optimize not only cryopreservation, but also IVM protocols for use in either germinal vesicle or metaphase-II oocytes, together with a comprehensive assessment of oocyte function and developmental competence to term. Despite current challenges, the burgeoning field of immature oocyte cryopreservation constitutes a promising option for cancer patients with impaired ovarian function. KEY WORDS: immature oocyte, fertility preservation, cryopreservation, cancer, in vitro maturation Why target immature oocytes in cancer patients?In the last few decades, the incidence of cancer and the likelihood of survival have risen across the globe. A little over 1 in 3 women in North America are diagnosed with cancer every year. This constitutes an overwhelming number of young women of reproductive age undergoing chemotherapy, radiotherapy, and other oncologic treatments; an estimated 9% of cancer survivors are 20-39 years old in the United States. Invasive cancers are treated aggressively through chemotherapy, radiotherapy and surgeries to remove cancerous cells and masses. Unfortunately such treatments create irreversible damage to the gonads and possible sterility (reviewed by Anchan and Ginsburg, 2010). Depending on the type and dose, oncological therapies may result in damage and/or loss of not only germ cells but also the supporting somatic compartments of the ovarian follicle. Due to the women's finite number of oocytes and the irreplaceable nature of the pool Int. J. Dev. Biol. 56: 919-929 (2012)

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“…However, it is well known that the vitrification procedure exerts a detrimental effect(s) on oocytes; for example, the most serious damage after vitrification is disorganization of spindles potentially caused by microtubule disassembly in vitrified oocytes [40]. Recently, paclitaxel treatment prior to vitrification has been shown to improve the developmental ability of mouse [41], human [42], bovine [43] and porcine [44] oocytes. Paclitaxel is a chemical that promotes formation of highly stable microtubules that resist depolymerization, thus preventing normal cell division and arresting the cell cycle at the M-phase in somatic cells [45,46].…”

Section: Discussionmentioning

confidence: 99%

Ethylene Glycol-supplemented Calcium-free Media Improve Zona Penetration of Vitrified Rat Oocytes by Sperm Cells

et al. 2010

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Abstract. Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for matured rat oocytes. To determine the optimal equilibration time, oocytes were equilibrated in 7.5% ethylene glycol (EG) + 7.5% dimethyl sulfoxide (DMSO) + 20% fetal calf serum (FCS) for 1, 4, 7 or 10 min at 24 C and then 15% EG + 15% DMSO + 0.5 M sucrose + 20% FCS for 1 min at 24 C before being plunged into liquid nitrogen on Cryotops. Oocytes exposed to equilibration medium for 4 min showed higher survival and cleavage rates after artificial activation than those of oocytes exposed for 1, 7 or 10 min. The survival and cleavage rates of vitrified oocytes after activation were 98.3 and 78.4%, respectively. However, the perivitelline spaces of most of the vitrified/warmed oocytes (6/168, 3.6%) could not be penetrated by sperm after in vitro fertilization, and cortical granule exocytosis (CGE) was observed in the oocytes. Therefore, the inhibitory effect of calcium and cryoprotectants in vitrification medium on CGE was examined. In most of the oocytes vitrified in calcium-free media, CGE was strongly suppressed independent of cryoprotectants. Oocytes vitrified in EG-supplemented calcium-free media showed high survival rates after warming (79.4%). After artificial activation, the cleavage and blastocyst formation rates of the oocytes were also high (72.8 and 23.1%, respectively). The zona penetration rate of vitrified/ warmed oocytes was dramatically improved by using EG-supplemented calcium-free media after in vitro fertilization (111/155, 63.9%). Thus, our data suggest that EG-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells. Key words: Cryopreservation, Oocytes, Rat, Vitrification (J. Reprod. Dev. 56: [169][170][171][172][173][174][175] 2010) ryopreservation of germ cells and reproductive organs is a useful and important technology for efficient production and maintenance of transgenic, mutant, knock-out and other animals. In particular, embryo and sperm cryopreservation has been routinely used for not only efficient production of experimental animals but also clinical medicine. On the other hand, the most desired germ cells for freezing or vitrification are matured oocytes because successfully cryopreserved oocytes can be used for in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) or somatic-cell nuclear transfer (SCNT) [1,2]. Although successful freezing or vitrification of oocytes has been reported in several mammalian species [3][4][5][6], the survival is generally low. Especially in rats, a high rate of successful vitrification of early-stage embryos has not been obtained until recent years, possibly because rat germ cells seem to be sens...

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Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution

Cited by 8 publications

References 0 publications

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

The use of immature oocytes in the fertility preservation of cancer patients: current promises and challenges

Ethylene Glycol-supplemented Calcium-free Media Improve Zona Penetration of Vitrified Rat Oocytes by Sperm Cells

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