Freezing of embryonic neural tissue and its transplantation in the rat brain

Houlé, John D.; Das, Gopal D.

doi:10.1016/0006-8993(80)90909-9

Cited by 45 publications

(3 citation statements)

References 6 publications

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Section: Introductionmentioning

confidence: 99%

“…duration of storage seems to have no effect on cell survival (3,14,28). However, the freezing and thawing procedures appear to result in a variable reduction of cell The increasing interest for intracerebral transplantation of fetal neural allo-and xenografts as a potential survival (3,4,14). This variability in cell survival seems not only to be related to variations in the freezing and therapy for Parkinson's disease (PD) and other neurodegenerative diseases has created a demand for a reliable thawing procedures but also to factors such as gestational age and species of the animal used.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of Porcine Fetal Ventral Mesencephalic Tissue for Intrastriatal Transplantation in Parkinson's Disease

Koopmans¹,

HogenEsch²,

Copray

et al. 2001

Cell Transplant

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In this study we examined the efficacy of cryopreserving porcine fetal mesencephalic tissue. After microscopical dissection of the ventral mesencephalon (VM) from E28 pig fetuses, the collection of explants was randomly divided into two equal parts. One part was directly prepared as cell suspension. The other part was stored in hibernation medium for less than 2 days and then cryopreserved as tissue fragments and stored in liquid nitrogen. After 2 weeks up to 1 year, these tissue fragments were thawed and processed as cell suspensions. After cell counting and assessment of viability, these cell suspensions were used to examine survival, morphology, and neurite formation of the dopaminergic neurons in cell culture as well as after intrastriatal implantation in 6-OHDA-lesioned rats. Comparison of cryopreserved with fresh VM cell suspensions showed no significant difference with respect to cell viability and the average number of living cells per VM explant. The morphology of cultured dopaminergic neurons after cryopreservation was identical to that of fresh cells. After intrastriatal implantation, survival and outgrowth of cryopreserved dopaminergic neurons as well as functional effects did not differ from those of fresh cells. In conclusion, the cryopreservation technique we used proves to be a reliably effective method for storing porcine fetal VM tissue.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Porcine Fetal Ventral Mesencephalic Tissue for Intrastriatal Transplantation in Parkinson's Disease

Koopmans¹,

HogenEsch²,

Copray

et al. 2001

Cell Transplant

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“…Finally, can cultured cells serve as donor material? It is kown that well-characterized cell lines possess characteristics of both cholin ergic and catecholaminergic cells [6], They synthesize, store and release primarily dopamine. Under some circumstances, they can also store, synthesize and release acetylcholine.…”

Section: Brain Graftsmentioning

confidence: 99%

Missing Pieces in the Epilepsy Puzzle

Ward¹

1985

Stereotact Funct Neurosurg

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Research regarding the basic mechanisms of the epilepsies has been limited and has generated few clues that have led to dramatic improvements in the surgical therapy of epilepsy. It is now known that the epileptic focus is characterized by a population of pacemaker neurons that fire autonomously in bursts. When this high frequency discharge recruits bursting activity in neighboring neurons, a propagating seizure occurs. Such data as well as other recent data in neurobiology indicate that new forms of surgical therapy may be forthcoming including brain transplantation as well as local application of transmitter compounds which may significantly improve the therapy of epilepsy.

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Embryonic neuronal transplantation

Fine

1984

BioEssays

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Freezing of embryonic neural tissue and its transplantation in the rat brain

Cited by 45 publications

References 6 publications

Cryopreservation of Porcine Fetal Ventral Mesencephalic Tissue for Intrastriatal Transplantation in Parkinson's Disease

Cryopreservation of Porcine Fetal Ventral Mesencephalic Tissue for Intrastriatal Transplantation in Parkinson's Disease

Missing Pieces in the Epilepsy Puzzle

Embryonic neuronal transplantation

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