Freezing Diluted Bovine Serum Albumin Standards Does Not Significantly Affect Standard Curves
Rachelle Sheets,
Bhavik Rajaboina,
Caitlin E. Bromberg
et al.
Abstract:Total protein isolation followed by quantitation is a common protocol in many laboratories. Quantitation is often done using a colorimetric assay such as the bicinchoninic acid (BCA) assay in which a change in the color of the BCA reagent is related to protein concentration. Extracted protein samples are compared to a standard curve made with dilutions of a protein standard such as bovine serum albumin (BSA) to determine their concentrations. A series of experiments was designed to determine the most reproduci… Show more
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