Freezing capacity of sperm on various type of superior bulls

Sukmawati, Eros; Arifiantini, Raden Iis; Purwantara, Bambang

doi:10.14334/jitv.v19i3.1079

Cited by 18 publications

(24 citation statements)

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“…Sorongbe et al (2019) reported that the MDA content of frozen buck semen with TEY diluent was 1.25±0.17 nmol/10 8 . Frozen semen of Limousin bull diluted using SMEY also showed low MDA levels (Sukmawati et al, 2014). MDA levels in the frozen semen of FH bull diluted using TEY were higher than those in this study (Asadpour et al, 2012).…”

Section: Tasj-36996contrasting

confidence: 56%

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The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Arif¹,

Maulana²,

Kaiin³

et al. 2022

Trop. Anim. Sci. J.

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Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris-egg yolk (TEY), Tris-egg yolk-skimmed milk (TEYSM), or Andromed ® . Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4 h. Following equilibration, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed ® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed ® diluent semen showed the lowest values compared with SMEY semen (p<0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed ® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.

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Section: Tasj-36996contrasting

confidence: 56%

“…MDA levels were tested using the thiobarbituric acid (TBA) sperm method (Anghel & Stela, 2010;Sukmawati et al, 2014). One milliliter of thawed semen was centrifuged at 1000 ×g for 10 min (Kitman-T24, Tommy, Japan).…”

Section: Malondialdehyde (Mda) Levels Evaluationmentioning

confidence: 99%

The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Arif¹,

Maulana²,

Kaiin³

et al. 2022

Trop. Anim. Sci. J.

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“…This study showed a decrease in the values of sperm viability that normally range between 18% and 23%. Sperm viability after freezing decreased around 20-35% [ 13 ]. The percentage of sperm viability in each dilution was good – sperm viability after thawing was >50%.…”

Section: Resultsmentioning

confidence: 99%

“…The MDA levels in Limousin bulls’ frozen semen with SMEY extender have been reported [ 13 ] as 0.52±0.25 nmol/10 8 . The researchers used Limousin bulls aged 4-8 years from different AI Centers.…”

Section: Resultsmentioning

confidence: 99%

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Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen

et al. 2020

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Background and Aim: Indonesia has two National Artificial Insemination centers and 17 Regional Artificial Insemination Centers. The frozen semen production techniques differed between the centers, including the type of diluent and semen dilution technique. The aim of the research was to compare the quality of frozen Limousin bull semen diluted using different techniques. Materials and Methods: Semen was collected from three sexually mature Limousin bulls using an artificial vagina. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen that had >70% motile sperm and <20% sperm abnormality was divided into three tubes and diluted with skim milk-egg yolk (SMEY) using three different dilution techniques: One-step dilution (100% SMEY with 8% glycerol) at room temperature ([RT] 20°C until 25°C) two-step dilution (50% SMEY without glycerol at RT, stored at 5°C; and 50% SMEY with 16% glycerol after 1 h stored at 5°C); and three-step dilution (50% SMEY without glycerol at RT, stored at 5°C; and 50% SMEY with 16% glycerol added twice at 1 h and 1.5 h after being stored at 5°C). The diluted semen was loaded into 0.25 mL mini straws, equilibrated, and frozen using a freezing machine. Sperm motility, viability, membranes, DNA integrity, and concentrations of malondialdehyde (MDA) and aspartate aminotransferase (AST) enzymes were evaluated after thawing. Results: The results showed that there were no significant differences in sperm motility and DNA integrity between dilutions (p>0.05). However, sperm viability and membrane intactness of one-step dilutions were higher than those of three-step dilutions. The concentrations of MDA and AST enzymes of sperm in one-step dilutions were lower than those of three-step dilutions (p<0.05). Conclusion: It was concluded that the one-step-dilution technique was better than three-step dilution for cryopreservation of Limousin bull semen.

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