Freeze-thaw stability of aluminum oxide nanoparticles

Trenkenschuh, Eduard; Frieß, Wolfgang

doi:10.1016/j.ijpharm.2021.120932

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…Therefore, ‘Z’ causes the electrostatic repulsion between the adjacent particles in a dispersion medium (39); higher magnitude of ‘Z’ (negative or positive) corresponds to higher magnitude of repulsive forces between the particles and thereby higher stability of the particles preventing aggregation/flocculation. Particles having zeta potentials > ± 30 mV are normally considered stable (40) and so the ENPs can be claimed to have moderate stability.…”

Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

Basu¹,

Ghosh²,

Sett³

et al. 2022

Preprint

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This study dealt with nanonization of eugenol, a major phytochemical present in basil leaf, which has pharmacological potential as an anti-bacterial agent. Eugenol nanoparticle (ENP) was synthesized by simple ultrasonic cavitation method through emulsification of hydrophobic eugenol into hydrophilic gelatin. Thus, the nanonization process made the water-insoluble eugenol to water-soluble nano-eugenol, making the nano-form bioavailable. The average size of the ENPs was 20-30 nm. Entrapment efficiency of eugenol within gelatin cap was about 80% of the eugenol, that was used as precursor in the nanonization reaction. In vitro release of eugenol from gelatin cap was slow and sustained over a period of five days. The ENP had higher anti-biofilm potency than eugenol for both formation and eradication of biofilm, formed by clinically relevant pathogen Pseudomonas aeruginosa. Minimal biofilm inhibitory concentration and minimal biofilm eradication concentration of ENPs were 2.0 and 4.0 mM respectively. In addition, the measurement of P. aeruginosa biofilm biomass, biofilm pellicle formation, biofilm thickness, amount of biofilm-forming extra-polymeric substance, cell surface hydrophobicity, cell swarming and twitching efficiencies, cellular morphology and biofilm formation in catheter demonstrated that the anti-biofilm efficacy of nano-eugenol was 30-40% higher than that of bulk eugenol. Thus, ENP can be used as a potential drug against pneumonia, a chronic infection in lung caused by P. aeruginosa, which is difficult to treat with antibiotics, due to natural intrinsic resistance of biofilm-formed cells to most antibiotics. The overall actions of ENP have been presented in the figure 1.

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Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

Basu¹,

Ghosh²,

Sett³

et al. 2022

Preprint

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“…Generally, particles of nanometer size implied a tremendous surface space and surface energy, which could cause both physical and chemical instability across diverse pH, thermal variations or gravitational conditions. 39 , 40 Therefore, it is crucial to evaluate the stability of STC@BBR-SANPs. Being an essential stability parameter, ZP is the electric potential of the slip/shear surface of a nanoparticle that is moving with an electric field.…”

Section: Discussionmentioning

confidence: 99%

Self-Assembled nanoparticles Combining Berberine and Sodium Taurocholate for Enhanced Anti-Hyperuricemia Effect

Qin¹,

Li²,

Cheng³

et al. 2023

IJN

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Propose Berberine (BBR) is extensively studied as an outstanding anti-hyperuricemia drug. However, the clinical application of BBR was limited due to its poor absorption and low bioavailability. Therefore, there is an urgent necessity to find a novel drug formulation to address the issues of BBR in clinical application. Methods Herein, we conducted the solubility, characterization experiments to verify whether BBR and sodium taurocholate (STC) self-assembled nanoparticles (STC@BBR-SANPs) could form. Furthermore, we proceeded the release experiment in vitro and in vivo to investigate the drug release effect. Finally, we explored the therapeutic effect of STC@BBR-SANPs on hyperuricemia (HUA) through morphological observation of organs and measurement of related indicators. Results The solubility, particle size, scanning electron microscopy (SEM), and stability studies showed that the stable STC@BBR-SANPs could be formed in the BBR-STC system at ratio of 1:4. Meanwhile, the tissue distribution experiments revealed that the STC@BBR-SANPs could accelerate the absorption and distribution of BBR. In addition, the pharmacology study demonstrated that both BBR and STC@BBR-SANPs exhibited favorable anti-HUA effects and nephroprotective effects, while STC@BBR-SANPs showed better therapeutic action than that of BBR. Conclusion This work indicated that STC@BBR-SANPs can be self-assembly formed, and exerts excellent uric acid-lowering effect. STC@BBR-SANPs can help to solve the problems of poor solubility and low absorption rate of BBR in clinical use, and provide a new perspective for the future development of BBR.

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“…Freezing is the critical stress for products stored as frozen liquids or lyophilized substances [ 97 ]. The F/T method has been used to evaluate the stability of different nanosystems.…”

Section: Pharmaceutical Applications Of Freeze-thawmentioning

confidence: 99%

“…In the case of aluminum oxide nanoparticles, the addition of potassium phosphate, sodium citrate buffers, or stabilizers such as PVA or gelatin A/B reduces the increased nanoparticle size after being subjected to 3 cycles of F/T, frozen at -1 ºC/min to -50 ºC and thawed at 1 ºC/min to 10 ºC with 90 min in both stages. Therefore, the authors identified that the fundamental formulation principles to preserve inorganic nanoparticles upon freezing are maintaining the pH during freezing and adding a suitable stabilizer [ 97 ].…”

Section: Pharmaceutical Applications Of Freeze-thawmentioning

confidence: 99%

Enhancing chemical and physical stability of pharmaceuticals using freeze-thaw method: challenges and opportunities for process optimization through quality by design approach

et al. 2023

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The freeze-thaw (F/T) method is commonly employed during the processing and handling of drug substances to enhance their chemical and physical stability and obtain pharmaceutical applications such as hydrogels, emulsions, and nanosystems (e.g., supramolecular complexes of cyclodextrins and liposomes). Using F/T in manufacturing hydrogels successfully prevents the need for toxic cross-linking agents; moreover, their use promotes a concentrated product and better stability in emulsions. However, the use of F/T in these applications is limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading, and drug release capacity), which depend on the optimization of process conditions and the kind and ratio of polymers, temperature, time, and the number of cycles that involve high physical stress that could change properties associated to quality attributes. Therefore, is necessary the optimization of F/T conditions and variables. The current research regarding F/T is focused on enhancing the formulations, the process, and the use of this method in pharmaceutical, clinical, and biological areas. The present review aims to discuss different studies related to the impact and effects of the F/T process on the physical, mechanical, and chemical properties (porosity, swelling capacity) of diverse pharmaceutical applications with an emphasis on their formulation properties, the method and variables used, as well as challenges and opportunities in developing. Finally, we review the experimental approach for choosing the standard variables studied in the F/T method applying the systematic methodology of quality by design.

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Freeze-thaw stability of aluminum oxide nanoparticles

Cited by 10 publications

References 44 publications

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

Anti-Biofilm Potential of Nanonized Eugenol againstPseudomonas aeruginosa

Self-Assembled nanoparticles Combining Berberine and Sodium Taurocholate for Enhanced Anti-Hyperuricemia Effect

Enhancing chemical and physical stability of pharmaceuticals using freeze-thaw method: challenges and opportunities for process optimization through quality by design approach

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