Freeze-thaw Assessment of Plants Based on Envelope Analysis of Stem Volume Water Content Sequence

Abstract

Background: Frost stress is an abiotic stressor for plant growth that impacts the health and the regional distribution of plants. The freeze-thaw characteristics of plants during the overwintering period help to understand relevant issues in plant physiology, including plant cold resistance and cold acclimation. Therefore, we aimed to develop a non-invasive instrument and method for accurate in situ detection of changes in stem freeze-thaw characteristics during the overwintering period. Results: A sensor was designed based on standing wave ratio method (SWR) to measure stem volume water content (StVWC). We were able to measure stem volume ice content (StVIC) and stem freeze-thaw rate of ice (StFTRI) during the overwintering period. The resolution of the StVWC sensor is less than 0.05 %, the mean absolute error and root mean square error are less than 1 %, and the dynamic response time is 0.296 s. The peak point of the daily change rate of the lower envelope of the StVWC sequence occurs when the plant enters and exits the overwintering period. The peak point can be used to determine the moment of freeze-thaw occurrence, whereas the time point corresponding to the moment of freeze-thaw coincides with the rapid transition between high and low ambient temperatures. In the field, the StVIC and StFTRI of Juniperus virginiana L., Lagerstroemia indica L. and Populus alba L. gradually increased at the beginning, fluctuated steadily during, and then gradually decreased by the end of the overwintering period. The StVIC and StFTRI also showed significant variability due to differences among the tree species and latitude.Conclusions: The StVWC sensor has good resolution, accuracy, stability, and sensitivity. The envelope changes of the StVWC sequence and the correspondence between the freeze-thaw moment and the ambient temperature indicate that the determination of the freeze-thaw moment based on the peak point of the daily change rate of the lower envelope is reliable. The results show that the sensor is able to monitor changes in the freeze-thaw characteristics of plants and effectively characterize freeze-thaw differences and cold resistance of different tree species. Furthermore, this is a cost-effective tool for monitoring freeze-thaw conditions during the overwintering period.