Freeze‐etch observations of rat lung

Belton, John C.; Branton, Daniel; Thomas, Heriberto V.; Mueller, Peter

doi:10.1002/ar.1091700408

Cited by 21 publications

(7 citation statements)

References 24 publications

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“…3). Similar lamellar structures have been demonstrated in lipid-water preparation (DEAMER et al, 1970), in phospholipid granules in the lung (BELTON et al, 1971), and in lecithin-water system (FLUCK et al, 1969) when prepared by freeze-etching methods.…”

Section: Methodssupporting

confidence: 74%

The Appearance of Lipid Cells in Freeze-Etched Preparations

Daikoku

Shinohara

Watanabe

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Methodssupporting

confidence: 74%

The Appearance of Lipid Cells in Freeze-Etched Preparations

Daikoku

Shinohara

Watanabe

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Such particles, believed to be protein incorporated into the lamellae, may play an important role in the maintenance of the structure of the concentric lamellae of type A MS. We believe that the very smooth concave depressions or convex bulges in type A MS correspond to type B MS; however, we were not able to observe cross-fractured surfaces of these areas. Freeze-fracture images of type C MS showed slightly particulate surfaces, which were similar to those of lamellar bodies of type I1 alveolar epithelial cells (Belton et al, 1971;Smith et al, 1972;Kikkawa and Manabe, 1978;Schulz et al, 1980). Some parts of type D MS showed rows of particles along their longitudinal axes.…”

Section: Freeze-fracture Evaluation Of Msmentioning

confidence: 78%

Ultrastructural, histochemical, and freeze‐fracture evaluation of multilamellated structures in human pulmonary alveolar proteinosis

et al. 1987

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Ultrastructural, histochemical, and freeze-fracture studies of material recovered by bronchoalveolar lavage from patients with pulmonary alveolar proteinosis revealed four types (A, B, C, and D) of multilamellated structures (MS). Type A, the major component, consisted of concentric, trilaminar structures which were composed of two electron-dense layers and a central lucent layer (5.7-7.5 nm in overall width) alternating with wider (25-30 nm) electron-lucent intervening layers. Type B MS were formed by concentric lamellae with a 5-5.3-nm periodicity. Type C MS were composed of wavy, electron-dense lamellae with a 4-4.5-nm periodicity. Type D MS were conglomerated masses of intricately arranged double or triple electron-dense layers (7.5-13.5 nm wide) alternating with wider (30-40-nm) electron-lucent layers. The electron-dense lamellae of type A, type C, and type D MS were stained with ruthenium red, the Thiéry method, and concanavalin A, indicating the presence of carbohydrate components. Freeze-fracture studies revealed smooth inner and outer surfaces in type A MS, with the fracture planes passing through the central parts of the trilaminar structures; the intervening layers contained 10-nm particles, which probably are proteins. Type B MS had smooth surfaces, and type C MS had slightly particulate surfaces; while type D MS showed tubular or polygonal structures, 350 nm wide, with rows of particles 7-8 nm in diameter. It is concluded that type A and type D MS contain proteins and carbohydrates, probably in the form of glycoproteins, as well as phospholipids, and are related to tubular myelin. Type B and type C MS are considered to contain mainly phospholipids; type C MS are also considered to contain carbohydrates and to be related to lamellar bodies of type II alveolar epithelial cells.

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“…This finding, however, is not in agreement with the previous reports by Kobayashi (1981aKobayashi ( ,b, 1982. According to him, lipid droplets in the Ito cells display concave or convex multilayered structures similar to those seen in the lamellar bodies of type I1 alveolar cells in the lungs (Belton et al, 1971). Kobayashi also concluded that these multilayered structures corresponded to myelinlike structures found in lipid droplets viewed in ultrathin sections.…”

Section: Discussionmentioning

confidence: 99%

Freeze‐replica study of lipid droplets in the ito cells of rat liver

Yamamoto

Ogawa

1984

Anat. Rec.

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The structure of lipid droplets in the Ito cells was studied by the freeze-replica method in vitamin A-treated rats. Most lipid droplets were fractured in planes through their lipid contents. Some of them were surrounded by a membrane structure that had intramembranous particles (IMP), which were more numerous on the protoplasmic face than the exoplasmic face. Those with the membrane containing IMP correspond to the membrane-bounded lipid droplets (lipid droplets surrounded by lysosomes) seen in the electron microscopic observation of conventional thin section preparations. There were no lipid droplets showing concave or convex multilayered structures in the Ito cells from properly fixed livers. Since these multilayered structures were observed only in the materials fixed by immersion with glutaraldehyde or fixed 40 minutes after the death of animals, it is likely that they represent an artifact, showing an autolytic change, caused by poor fixation or postmortem change.

show abstract

Freeze‐etch observations of rat lung

Cited by 21 publications

References 24 publications

The Appearance of Lipid Cells in Freeze-Etched Preparations

The Appearance of Lipid Cells in Freeze-Etched Preparations

Ultrastructural, histochemical, and freeze‐fracture evaluation of multilamellated structures in human pulmonary alveolar proteinosis

Freeze‐replica study of lipid droplets in the ito cells of rat liver

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