Freeze-drying of nanostructure lipid carriers by different carbohydrate polymers used as cryoprotectants

Varshosaz, Jaleh; Eskandari, Sharareh; Tabbakhian, Majid

doi:10.1016/j.carbpol.2012.01.051

Cited by 96 publications

(41 citation statements)

References 27 publications

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“…In view of this, nanostructured lipid carrier (NLC) appear to be an attractive approach for the delivery of highly lipophilic drugs such as ART as NLCs have advantages over all other colloidal systems -SLNs, SLMs, liposomes, nanoemulsions, microemulsions [15][16][17][18][19][20]. This is because the majority of drugs have higher solubility in liquid lipids (oils) rather than solid lipids.…”

Section: Introductionmentioning

confidence: 99%

Development of artemether-loaded nanostructured lipid carrier (NLC) formulation for topical application

Nnamani

Hansen

Windbergs

et al. 2014

International Journal of Pharmaceutics

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NLC topical formulation as an alternative to oral and parenteral (IM) delivery of artemether (ART), a poorly water-soluble drug was designed. A Phospholipon 85G-modified Gelucire 43/01 based NLC formulation containing 75 % Transcutol was chosen from DSC studies and loaded with gradient concentration of ART (100-750 mg). ART-loaded NLCs were stable (-22 to -40 mV), polydispersed (0.4 to 0.7) with d90 size distribution range of 247 to 530 nm without microparticles up to one month of storage. The encapsulation efficiency (EE %) for ART in the NLC was concentration independent as 250 mg of ART loading achieved ~61%. DSC confirmed molecular dispersion of ART due to low matrix crystallinity (0.028 J/g). Ex vivo study showed detectable ART amounts after 20 h which gradually increased over 48 h achieving ~26 % cumulative amount permeated irrespective of the applied dose. This proves that ART permeates excised human epidermis, where the current formulation served as a reservoir to gradually control drug release over an extended period of time. Full thickness skin study therefore may confirm if this is a positive signal to hope for a topical delivery system of ART.

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Section: Introductionmentioning

confidence: 99%

Development of artemether-loaded nanostructured lipid carrier (NLC) formulation for topical application

Nnamani

Hansen

Windbergs

et al. 2014

International Journal of Pharmaceutics

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“…Tsai et al (2012) have reported sizes below 100 nm. Lipid nanoparticles of sizes between 100 and 200 nm have been reported by a few researchers (Gupta and Vyas 2012;Noack et al 2012;Priano et al 2011;Varshosaz et al 2012). Others have reported SLNs with sizes varying from 200 to above 500 nm (de Souza et al 2012;Jia et al 2012;Xie et al 2011;Yang et al 2013).…”

Section: Photon Correlation Spectroscopymentioning

confidence: 92%

“…Sterilization and freeze drying of SLNs can reduce the zeta potential. The presence of cryoprotectant can also influence the zeta potential of SLNs (Cavalli et al 1997;Schwarz and Mehnert 1997;Soares et al 2013;Varshosaz et al 2012). Formulation parameters such as surfactants and the lipid matrix also affect zeta potential determinations (Kovacevic et al 2014(Kovacevic et al , 2011.…”

Section: Surface Chargementioning

confidence: 97%

Characterization

Shah¹,

Eldridge²,

Palombo³

et al. 2014

Lipid Nanoparticles: Production, Characterization and Stability

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Lipid nanoparticles are known to present three features-a solid nature, lipid matrix and colloidal (or smaller) particle size. These features are theorized to impart controlled drug release, biocompatibility and improved drug dissolution to the colloidal carriers. Appropriate characterization of lipid nanoparticle formulations is required to allow for the development of dispersions with the desired properties for the intended application. The structural complexity and the colloidal size of the lipid nanoparticle dispersions make characterization a difficult task. The colloidal size of the particles alters physical features (e.g., increasing solubility and the tendency to form supercooled melts). The properties of bulk materials such as polymorphism and crystallinity may drastically change when these materials are dispersed into nanoparticles. Further complicating characterization is the potential co-existence of other colloidal structures such as micelles, vesicles and emulsions in the dispersions. A detailed characterization scheme is required to investigate the structure and behavior of these complex colloidal carriers. This chapter introduces a number of methods commonly applied for characterization of lipid nanoparticle dispersions. The main focus of the chapter is lipid nanoparticles, however the methods are often generic and occasional mention of their use in other colloidal structures is made.

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“…Aliquots were withdrawn at time intervals of 0, 10, 20, 30, 40, 50, 60, 90, 120, 150, 180, 210, 240 min and replaced with equal volume of fresh buffer solution. [11][12][13][14] The aliquots withdrawn were filtered and the absorbance was recorded using UV-visible spectrophotometer (Shimadzu 1800, Japan). The concentration of the drug in the aliquots and % drug permeation were determined by using PCP Disso V2.08 software.…”

Section: In-vitro Drug Diffusion Studymentioning

confidence: 99%

Development and Evaluation of Amlodipine Besylate Nanogel

Kamble¹,

Dange²,

Bhalerao³

et al. 2015

j bionanosci

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The present study aimed at preparing nanostructured lipid carrier gel for intranasal drug delivery system of amlodipine besylate, an antihypertensive drug. The amlodipine besylate loaded nanostructured lipid carriers were prepared by emulsion solvent diffusion and evaporation method followed by ultrasonication. The properties of nanostructured lipid carriers such as particle size, zeta potential and entrapment efficiency were investigated. The nanostructured lipid carrier dispersion was suitably gelled and characterized for drug content, pH, in-vitro and ex-vivo drug diffusion. FTIR and DSC studies showed no chemical interaction between amlodipine besylate and excipients in the formulation. The particle size, zeta potential and entrapment efficiency amlodipine besylate loaded nanostructured lipid carriers were found to be 424.3 nm, −14.2 mV and 52 5 ± 2 31% respectively. The drug content of amlodipine nanostructured lipid carriers gel was found to be 96 5 ± 0 5%. Toxicological study carried out on sheep nasal mucosa showed no evidence of toxicity of nanostructured lipid carrier based gel. Amlodipine nanostructured lipid carriers based gel showed a good potential for prolonged release as compared with pure drug solution and nanostructured lipid carrier dispersion.

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Freeze-drying of nanostructure lipid carriers by different carbohydrate polymers used as cryoprotectants

Cited by 96 publications

References 27 publications

Development of artemether-loaded nanostructured lipid carrier (NLC) formulation for topical application

Development of artemether-loaded nanostructured lipid carrier (NLC) formulation for topical application

Characterization

Development and Evaluation of Amlodipine Besylate Nanogel

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