Freeze Drying (Lyophilization) of Red Blood Cells

Arav, Amir; Natan, Dity

doi:10.1097/ta.0b013e31821a6083

Cited by 16 publications

(6 citation statements)

References 14 publications

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“…50 Use of lyophilized blood products may represent an opportunity in this area. 51 Freeze dried plasma has received renewed interest in the military setting and is already undergoing successful PH use in austere conditions by the Israeli armed forces. 52 Lessons learned from ongoing work in this area will be vital.…”

Section: Discussionmentioning

confidence: 99%

Pretrauma Center Red Blood Cell Transfusion Is Associated With Reduced Mortality and Coagulopathy in Severely Injured Patients With Blunt Trauma

Brown¹,

Cohen

Minei

et al. 2015

Annals of Surgery

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Objective To evaluate the association of pretrauma center (PTC) red blood cell (RBC) transfusion with outcomes in severely injured patients. Background Hemorrhage remains a major driver of mortality. Little evidence exists supporting PTC interventions to mitigate this. Methods Blunt injured patients in shock arriving at a trauma center within 2 hours of injury were included from the Glue Grant database. Subjects were dichotomized by PTC RBC transfusion. Outcomes included 24-hour mortality, 30-day mortality, and trauma-induced coagulopathy [(TIC), admission international normalized ratio >1.5]. Cox regression and logistic regression determined the association of PTC RBC transfusion with outcomes. To address baseline differences, propensity score matching was used. Results Of 1415 subjects, 50 received PTC RBC transfusion. Demographics and injury severity score were similar. The PTC RBC group received 1.3 units of RBCs (median), and 52% were scene transports. PTC RBC transfusion was associated with a 95% reduction in odds of 24-hour mortality [odds ratio (OR) = 0.05; 95% confidence interval (CI), 0.01–0.48; P < 0.01], 64% reduction in the risk of 30-day mortality [hazard ratio = 0.36; 95% CI, 0.15–0.83; P = 0.02], and 88% reduction in odds of TIC (OR = 0.12; 95% CI, 0.02–0.79; P = 0.03). The matched cohort included 113 subjects (31% PTC RBC group). Baseline characteristics were similar. PTC RBC transfusion was associated with a 98% reduction in odds of 24-hour mortality (OR = 0.02; 95% CI, 0.01–0.69; P = 0.04), 88% reduction in the risk of 30-day mortality (hazard ratio = 0.12; 95% CI, 0.03–0.61; P = 0.01), and 99% reduction in odds of TIC (OR = 0.01; 95% CI, 0.01–0.95; P = 0.05). Conclusions PTC RBC administration was associated with a lower risk of 24-hour mortality, 30-day mortality, and TIC in severely injured patients with blunt trauma, warranting further prospective study.

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Section: Discussionmentioning

confidence: 99%

Pretrauma Center Red Blood Cell Transfusion Is Associated With Reduced Mortality and Coagulopathy in Severely Injured Patients With Blunt Trauma

Brown¹,

Cohen

Minei

et al. 2015

Annals of Surgery

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show abstract

“…Lyophilization of red blood cells has been hampered by damage to the cells without cryoprotectants such as glycerol; however, significant progress has been made using novel processes that allow for small volumes of freeze-dried red blood cells with acceptable functional rehydration of cells [126]. Technology exists to freezedry platelets as well; however, the limiting factor has been safety concerns.…”

Section: Freeze-dried Productsmentioning

confidence: 99%

Prehospital Resuscitation

Deeb

Brown

2020

Trauma Induced Coagulopathy

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“…Initial attempts took one of two approaches. Some researchers opted for drying cells that lack nuclear DNA in them, such as red blood cells and platelets [101][102][103][104][105][106]. Following this approach, the aim was to maintain the integrity of the membranes and cellular organeles and proteins, and thus the biological activity of these cells.…”

Section: Drying Other Cell Typesmentioning

confidence: 99%

Exploring dry storage as an alternative biobanking strategy inspired by Nature

Saragusty

Loi

2019

Theriogenology

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Close to 25% of species in the animal kingdom face the risk of extinction. A variety of stresses, largely anthropogenic in nature, and lack of sufficient resources and political support to counteract this biodiversity crisis, suggest that we will witness the extinction of many thousands of species in the coming years. Unless we act now to, at least, preserve samples from each and every threatened species, these extinct species will be lost forever. To preserve record of the world's biodiversity, establishment of genome resource banks is thus desirable. Presently, biobanking is done almost exclusively by cryopreservation, followed by maintenance of the samples under liquid nitrogen. Cryopreservation has satisfactory efficiency but it comes with a host of problems. The process is also highly species-specific. Like in many other walks of life, we can search Nature for better alternatives. When longterm preservation is desired, Nature opted for controlled drying rather than in water preservation via freezing. Nature also created an assortment of materials that protect organisms from damages during the drying and rehydration processes. Once dry, these organisms can survive extended periods of time and be resistant to extreme environmental stressors. Over the past 70 years researchers attempted applying this idea to preserved desiccation-sensitive mammalian cells in the dry form. Desiccation is experiencing increased interest in recent years with some exciting developments. Presented here an overview of dry preservation and its possible applications towards establishment of dry biobanks as part of our endeavour to preserve the world's biodiversity.

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Freeze Drying (Lyophilization) of Red Blood Cells

Cited by 16 publications

References 14 publications

Pretrauma Center Red Blood Cell Transfusion Is Associated With Reduced Mortality and Coagulopathy in Severely Injured Patients With Blunt Trauma

Pretrauma Center Red Blood Cell Transfusion Is Associated With Reduced Mortality and Coagulopathy in Severely Injured Patients With Blunt Trauma

Prehospital Resuscitation

Exploring dry storage as an alternative biobanking strategy inspired by Nature

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