Freeze-drying and analysis of plant and other biological material

Sumere, C. van; Geiger, Hans; Bral, D.; Fockenier, G.; Casteele, Karel Vande; Martens, Miet; Hanselaer, R.; Gevaert, L.

doi:10.1016/0003-2697(83)90210-5

Cited by 12 publications

(2 citation statements)

References 9 publications

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“…However, freezing, as well as conventional freeze-drying of prefrozen plant tissue, has been reported to induce dramatic changes in total and relative concentrations of Salix and Populus phenolic glycosides Lindroth and Pajutee, 1987;Julkunen-Tiitto and Tahvanainen, 1989). Furthermore, compounds of low or intermediate molecular weight may be partly removed from plant material during freezedrying by high vacuum (Van Sumere et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Effect of Sample Preparation Method on Birch (Betula pendula Roth) Leaf Phenolics

Keinänen

Julkunen‐Tiitto

1996

J. Agric. Food Chem.

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Sample preparation methods for high-performance liquid chromatographic analysis of birch leaf phenolics were compared. The methods tested were: (1) air-drying at ambient temperature, (2) oven-drying at 40 °C, (3) oven-drying at 80 °C, (4) freeze-drying, prefreezing with liquid N2, (5) freeze-drying, prefreezing at −18 °C, (6) freeze-drying without prefreezing, (7) storing frozen for 12 days without drying, and (8) immediate extraction of fresh samples. Although there were significant differences among methods in absolute concentrations of phenolics, none of the methods altered considerably the phenolic profile of birch leaves. For quantitative analysis, the samples should be analyzed immediately. Alternatively, the samples may be stored fresh frozen, at least for a short time. Among the drying methods, the highest concentrations of phenolics were obtained by freeze-drying of slowly frozen leaves (method 5). Freeze-drying without prefreezing resulted in significantly lower concentrations of flavonoid glycosides than method 5. Oven-drying did not prove to be a good alternative to conventional freeze-drying, nor did it show any advantage over simple air-drying. Keywords: Betula pendula; sample preparation; drying methods; flavonoid glycosides; phenolic compounds

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Section: Introductionmentioning

confidence: 99%

Effect of Sample Preparation Method on Birch (Betula pendula Roth) Leaf Phenolics

Keinänen

Julkunen‐Tiitto

1996

J. Agric. Food Chem.

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“…In this respect, van Sumere et al [183] concluded that the freeze-drying of biological material, which is to be quantitatively analysed (micro-amount level) for compounds of low or intermediate molecular weight, should either be omitted or handled under strict control. This is because such compounds as amino acids, sugars, flavonids, glycosides, coenzymes, peptides, etc., might be removed from concentrates and/or the ground biological material by the high vacuum [183].…”

Section: Organic Compoundsmentioning

confidence: 99%