Freeze dried cross linking free biodegradable composites with microstructures for tissue engineering and drug delivery application

Joshy, M.I. Ahymah; Kolanthai, Elayaraja; Sakthivel, N.; Shanthini, G.M.; Kalkura, S. Narayana

doi:10.1016/j.msec.2012.09.016

Cited by 12 publications

(7 citation statements)

References 29 publications

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“…Among these three components, scaffolds play significant roles since they maintain the transplanted cells and lead their functions effectively [ 8 – 11 ]. For the artificial bone transplant, materials or the device must be non-toxic in interaction with body function [ 12 , 13 ]. Hydroxyapatite (HA) (Ca10(PO 4 )6(OH)2) is known as the most biocompatible replacement biomaterial among the developed artificial bones, and it is a constituent of 70% of human [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

A comparison study on the behavior of human endometrial stem cell-derived osteoblast cells on PLGA/HA nanocomposite scaffolds fabricated by electrospinning and freeze-drying methods

et al. 2018

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BackgroundAn engineered tissue structure is an artificial scaffold combined with cells and signaling factors. Among various polymers, the polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties. The aim of this study was to study the behavior of human endometrial stem cell (hEnSC)-derived osteoblast cells cultured on PLGA/HA nanocomposite scaffolds.MethodshEnSCs were isolated and exposed to osteogenic media for 21 days. Differentiated cells were cultured on PLGA/HA synthetic scaffolds. The PLGA/HA-based nanocomposite scaffolds were fabricated using either electrospinning or freeze-drying methods. Behavior of the cells was evaluated a week after seeding hEnSC-derived osteoblast-like cells on these scaffolds. Osteogenesis was investigated in terms of alkaline phosphatase activity, gene expression, immunocytochemistry (ICC), proliferation, and scanning electron microscopy (SEM). Moreover, scaffold properties, such as pore size and morphology of the cells, onto the scaffolds were evaluated using SEM. Furthermore, biocompatibility of these scaffolds was confirmed by 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.ResultsThe matrix mineralization was proved by alizarin red staining, and the osteogenic media-treated cultures positively expressed osteocalcin and osteopontin markers. Moreover, qRT-PCR results confirmed the positive gene expression of osteopontin and osteonectin in the differentiated osteoblast-like cells. The results of behavior assessment of the cultured cells on electrospinning and freeze-dried scaffolds showed that the behavior of the cultured cells on the freeze-dried PLGA/HA scaffolds was significantly better than the electrospinning PLGA/HA scaffolds.ConclusionIt has been shown that the freeze-dried PLGA/HA nanocomposite scaffolds can appropriately support the attachment and proliferation of the differentiated osteoblast cells and are a suitable candidate for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

A comparison study on the behavior of human endometrial stem cell-derived osteoblast cells on PLGA/HA nanocomposite scaffolds fabricated by electrospinning and freeze-drying methods

et al. 2018

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“…Joshy et al (2013) [59] developed non-toxic, versatile and cost effective biodegradable laminated polymer composites comprising agarose (A), gelatin (G) and HAp without any crosslinking agents. Addition of HAp onto A, G, AG strongly influenced the swelling behaviour, drug release and haemolytic activity.…”

Section: Agarose-gelatin-hydroxyapatite Scaffoldsmentioning

confidence: 99%

A Review on Biodegradable Polymeric Materials Striving Towards the Attainment of Green Environment

Jayanth¹,

Kumar

Nayak

et al. 2017

J Polym Environ

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“…Collagen, gelatin, and chitosan have been studied and applied commonly . Collagen can be synthesized with glycosaminoglycans to form porous sponges that are applied in bone tissue engineering because these materials can simulate the artificial extracellular matrix and render good infiltration to the cells .…”

Section: Introductionmentioning

confidence: 99%

Chitosan/gelatin porous bone scaffolds made by crosslinking treatment and freeze‐drying technology: Effects of crosslinking durations on the porous structure, compressive strength, and in vitro cytotoxicity

Lou

Wen

Lin

2015

J of Applied Polymer Sci

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In this study, freezing was used to separate a solute (polymer) and solvent (deionized water). The polymer in the ice crystals was then crosslinked with solvents, and this diminished the linear pores to form a porous structure. Gelatin and chitosan were blended and frozen, after which crosslinking agents were added, and the whole was frozen again and then freeze-dried to form chitosan/gelatin porous bone scaffolds. Stereomicroscopy, scanning electron microscopy, compressive strength testing, porosity testing, in vitro biocompatibility, and cytotoxicity were used to evaluate the properties of the bone scaffolds. The test results show that both crosslinking agents, glutaraldehyde (GA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were able to form a porous structure. In addition, the compressive strength increased as a result of the increased crosslinking time. However, the porosity and cell viability were not correlated with the crosslinking times. The optimal porous and interconnected pore structure occurred when the bone scaffolds were crosslinked with GA for 20 min. It was proven that crosslinking the frozen polymers successfully resulted in a division of the linear pores, and this resulted in interconnected multiple pores and a compressively strong structure. The 48-h cytotoxicity did not affect the cell viability. This study successfully produced chitosan/gelatin porous materials for biomaterials application.

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Freeze dried cross linking free biodegradable composites with microstructures for tissue engineering and drug delivery application

Cited by 12 publications

References 29 publications

A comparison study on the behavior of human endometrial stem cell-derived osteoblast cells on PLGA/HA nanocomposite scaffolds fabricated by electrospinning and freeze-drying methods

A comparison study on the behavior of human endometrial stem cell-derived osteoblast cells on PLGA/HA nanocomposite scaffolds fabricated by electrospinning and freeze-drying methods

A Review on Biodegradable Polymeric Materials Striving Towards the Attainment of Green Environment

Chitosan/gelatin porous bone scaffolds made by crosslinking treatment and freeze‐drying technology: Effects of crosslinking durations on the porous structure, compressive strength, and in vitro cytotoxicity

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