Freezability of Iberian ibex (Capra pyrenaica) spermatozoa according to the glycerolization temperature and plasma testosterone concentration

Coloma, M.A.; Gómez-Brunet, A.; Velázquez, Rosario; Toledano-Dı́az, A.; Santiago-Moreno, J.

doi:10.1016/j.cryobiol.2010.07.005

Cited by 15 publications

(10 citation statements)

References 45 publications

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“…In addition, it would be possible to add the glycerol at 30 °C since there were no differences between adding glycerol at 30 or 5 °C, making easier the work in field conditions. These results agree with those from Coloma et al [4] in bucks, who reported no effect of the glycerolization temperature.…”

Section: Discussionsupporting

confidence: 93%

Improved cryopreservation protocol for Blanca-Celtibérica buck semen collected by electroejaculation

et al. 2013

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The collection of sperm samples by electroejaculation (EE) leads to an increase of the production of seminal plasma which could modify the tolerance of spermatozoa to the cryopreservation procedure. This study aims to compare a standard sperm cryopreservation protocol for samples collected by artificial vagina (AV) with the same protocol and modifications to this for samples obtained by EE. Semen from six males of Blanca-Celtibérica goat breed was collected by AV (control) and EE, and three experiments were conducted. In Experiment 1, it was examined the effects of egg yolk concentration contained in freezing extender (0%, 1.5%, 10% and 20% of egg yolk); in Experiment 2, it was evaluated the cooling rate from 30 to 5 °C (fast: 10 min and slow: 90 min) and the temperature of glycerol addition (30 and 5 °C); and in Experiment 3, it was examined the time of equilibration at 5 °C (0, 1, 2 or 3h). A heterologous in vitro fertilization test was carried out in order to compare the fertility of control samples with that resulting from the EE protocol which showed the highest sperm quality. Results showed greater sperm motility parameters after thawing for control samples cryopreserved in standard conditions in the three experiments. For samples collected by EE, extender with 20% egg yolk, a slow cooling rate and a longer equilibration time (3h) provided higher sperm quality, and no differences were observed between temperatures of glycerol addition. Samples collected by EE and cryopreserved with the protocol which yielded the best sperm quality after thawing showed higher fertility compared to AV.

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Section: Discussionsupporting

confidence: 93%

Improved cryopreservation protocol for Blanca-Celtibérica buck semen collected by electroejaculation

et al. 2013

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“…2000), and differences in the toxic effect of cryoprotectants might be expected depending on the level of circulating hormones. The relationship between the concentrations of these hormones and cryopreservation success may be linear, unlike in certain mammalian species (Coloma et al. 2010a).…”

Section: Discussionmentioning

confidence: 99%

“…Testosterone may affect sperm membrane properties, as reported in certain mammalian species (Ballesteros et al 1983;Purohit et al 2000), and differences in the toxic effect of cryoprotectants might be expected depending on the level of circulating hormones. The relationship between the concentrations of these hormones and cryopreservation success may be linear, unlike in certain mammalian species (Coloma et al 2010a). Alternatively, this protective effect might be explained by hormone-related changes in seminal plasma composition.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of the length of the photoperiod on rooster sperm quality is exerted, at least in part, via increases in the plasma testosterone concentration (Thurston and Korn 2000). However, the relationship between the latter and the efficiency of cryopreservation may not be perfectly correlated; for instance in some mammalian species, high plasma testosterone concentrations during the pre‐mating season have a negative effect on the efficiency of sperm cryopreservation (Coloma et al. 2010a).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Season on the Freezability of Free‐Range Poultry Semen

Santiago-Moreno

Castaño

Toledano-Dı́az

et al. 2011

Reprod Domestic Animals

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The purpose of the present study was to examine the seasonal variation in freezing damage in free-range rooster sperm. Over a period of 1 year, heterospermic semen samples were collected weekly by massage from the roosters of 14 Spanish chicken breeds, all housed under natural photoperiod and climatic conditions. All samples were frozen in straws using DMA as a cryoprotectant, placing them first in nitrogen vapour and then plunging them into liquid nitrogen. No seasonal effects on fresh sperm quality were found. Neither did season affect the percentage of viable frozen-thawed spermatozoa nor the percentage with an intact acrosome. However, the collection season influenced (p < 0.05) most frozen-thawed sperm motility values. The percentage of immotile frozen-thawed spermatozoa was lower (p < 0.05) in spring-collected sperm than in summer- or autumn-collected samples. The percentage of spermatozoa showing progressive motility was higher in spring-collected sperm compared with winter-, summer- or autumn-collected samples (p < 0.05). The curvilinear velocity (VCL), straight-line velocity (VSL) and average path velocity (VAP) values of spring-collected sperm were also higher (p < 0.05). In conclusion, spring would appear to be the best season for collecting and freezing the semen of free-range Mediterranean chicken breeds.

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“…Endocrine status may also alter the resistance of sperm cells to freeze-thawing. It has been suggested that increased plasma testosterone level has a negative effect on the cryosurvival of Capra ibex spermatozoa (Coloma et al, 2010). Although testosterone levels were not measured in the present study, it is well known that, in rams, testosterone levels are much higher during the breeding season, in comparison to the non-breeding season (Ali and Taha 2012;Aller et al, 2012;Zamiri et al, 2010).…”

Section: Discussionmentioning

confidence: 51%

Investigation of the changes observed in scrotal circumference, and native and post-thaw semen characteristics in karayaka rams during the breeding and nonbreeding seasons

Kulaksız

Şen

2019

J Hellenic Vet Med Soc

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The aim of the present study was to investigate and compare the impact of the breeding (BS) and non-breeding (NBS) seasons on scrotal circumference, and native and post-thaw semen characteristics in Karayaka rams. Six (6) Karayaka rams were used as semen donors, and ejaculates were collected with the aid of an artificial vagina during the BS (October-November) and NBS (April-May). Interestingly, sperm freezability was found to be higher in the NBS, when compared to the BS, and the sperm motility rate (36.00±2.74%, 55.0±3.24%) and percentage of abnormal sperm (44.00±1.90%, 57.00±2.09%) were found to significantly differ with season (P<0.05). In conclusion, the post-freezing sperm motility was better during the non-breeding season. Post-thaw sperm abnormality was higher during non-breading season. However, if ram semen is used fresh for AI, it can be collected and used during both during the breeding and non-breeding season.

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Freezability of Iberian ibex (Capra pyrenaica) spermatozoa according to the glycerolization temperature and plasma testosterone concentration

Cited by 15 publications

References 45 publications

Improved cryopreservation protocol for Blanca-Celtibérica buck semen collected by electroejaculation

Improved cryopreservation protocol for Blanca-Celtibérica buck semen collected by electroejaculation

Influence of Season on the Freezability of Free‐Range Poultry Semen

Investigation of the changes observed in scrotal circumference, and native and post-thaw semen characteristics in karayaka rams during the breeding and nonbreeding seasons

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