Freeform 3D Bioprinting Involving Ink Gelation by Cascade Reaction of Oxidase and Peroxidase: A Feasibility Study Using Hyaluronic Acid-Based Ink

Sakai, Shinji; Harada, Ryohei; Kotani, Takashi

doi:10.3390/biom11121908

Cited by 8 publications

(11 citation statements)

References 54 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to traditional bioprinting, freeform bioprinting allows for fabricating soft structures with good shape fidelity. Sakai et al prepared a phenol derivative hyaluronic acid (HA-Ph) bioink to print a soft hydrogel pattern using enzyme-based cross-linking of choline oxidase (COD) and HRP . The bioink composition included the HA-Ph, choline chloride, HRP, and the slurry support bath made of a xanthan gum (XG) and COD mixture.…”

Section: D Bioprinting Of Ha-based Bioinksmentioning

confidence: 99%

“…(B–L) Photographic images of the printed (HA-Ph) structures before and after PBS wash (top view - B, H, D, and J; side view - C, I, E, and K) and side views (F, L) of PBS-soaked printed constructs after day 8 and day 9 (scale bar: 5 mm). Reproduced with permission from ref . Copyright 2021 MDPI.…”

Section: D Bioprinting Of Ha-based Bioinksmentioning

confidence: 99%

“…Sakai et al prepared a phenol derivative hyaluronic acid (HA-Ph) bioink to print a soft hydrogel pattern using enzyme-based cross-linking of choline oxidase (COD) and HRP. 73 The bioink composition included the HA-Ph, choline chloride, HRP, and the slurry support bath made of a xanthan gum (XG) and COD mixture. Figure 6 represents the cylindrical and conical constructs of 15 mm diameter/10 mm height and 20 mm diameter/10 mm height printed using a freeform extrusion-based bioprinting technique.…”

Section: B-viii) (B-ix) Shows An Sem Image Of the Developed Hydrogel ...mentioning

confidence: 99%

See 2 more Smart Citations

Hyaluronic Acid as Bioink and Hydrogel Scaffolds for Tissue Engineering Applications

Sekar

Suresh

Zennifer

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Bioprinting is an additive manufacturing technique that focuses on developing living tissue constructs using bioinks. Bioink is crucial in determining the stability of printed patterns, which remains a major challenge in bioprinting. Thus, the choices of bioink composition, modifications, and cross-linking methods are being continuously researched to augment the clinical translation of bioprinted constructs. Hyaluronic acid (HA) is a naturally occurring polysaccharide with the repeating unit of Nacetyl-glucosamine and D-glucuronic acid disaccharides. It is present in the extracellular matrix (ECM) of tissues (skin, cartilage, nerve, muscle, etc.) with a wide range of molecular weights. Due to the nature of its chemical structure, HA could be easily subjected to chemical modifications and cross-linking that would enable better printability and stability. These interesting properties have made HA an ideal choice of bioinks for developing tissue constructs for regenerative medicine applications. In this Review, the physicochemical properties, reaction chemistry involved in various cross-linking strategies, and biomedical applications of HA have been elaborately discussed. Further, the features of HA bioinks, emerging strategies in HA bioink preparations, and their applications in 3D bioprinting have been highlighted. Finally, the current challenges and future perspectives in the clinical translation of HA-based bioinks are outlined.

show abstract

Section: D Bioprinting Of Ha-based Bioinksmentioning

confidence: 99%

Section: D Bioprinting Of Ha-based Bioinksmentioning

confidence: 99%

Section: B-viii) (B-ix) Shows An Sem Image Of the Developed Hydrogel ...mentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronic Acid as Bioink and Hydrogel Scaffolds for Tissue Engineering Applications

Sekar

Suresh

Zennifer

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Recently, polymer materials are increasingly applied in many areas, including medicine and pharmacy [ 1 , 2 ]. These materials are applied in preparation of wound dressings [ 3 ], medical equipment [ 4 ], artificial prostheses [ 5 ] or organs [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Studies on PVP-Based Hydrogel Polymers as Dressing Materials with Prolonged Anticancer Drug Delivery Function

et al. 2023

View full text Add to dashboard Cite

Tamoxifen is a well-known active substance with anticancer activity. Currently, many investigations are performed on the development of carriers that provide its effective delivery. Particular attention is directed toward the formation of cyclodextrin–drug complexes to provide prolonged drug delivery. According to our knowledge, carriers in the form of polyvinylpyrrolidone (PVP)/gelatin-based hydrogels incorporated with β-cyclodextrin–tamoxifen complexes and additionally modified with nanogold have not been presented in the literature. In this work, two series of these materials have been synthesized—with tamoxifen and with its complex with β-cyclodextrin. The process of obtaining drug carrier systems consisted of several stages. Firstly, the nanogold suspension was obtained. Next, the hydrogels were prepared via photopolymerization. The size, dispersity and optical properties of nanogold as well as the swelling properties of hydrogels, their behavior in simulated physiological liquids and the impact of these liquids on their chemical structure were verified. The release profiles of tamoxifen from composites were also determined. The developed materials showed swelling capacity, stability in tested environments that did not affect their structure, and the ability to release drugs, while the release process was much more effective in acidic conditions than in alkaline ones. This is a benefit considering their use for anticancer drug delivery, due to the fact that near cancer cells, there is an acidic environment. In the case of the composites containing the drug–β-cyclodextrin complex, a prolonged release process was achieved compared to the drug release from materials with unbound tamoxifen. In terms of the properties and the composition, the developed materials show a great application potential as drug carriers, in particular as carriers of anticancer drugs such as tamoxifen.

show abstract

“…Because the bath holds the form of the printed part, embedded 3D printing expands the materials space to include lower-viscosity materials, expands the toolpath space to include vertical lines instead of being constrained to x – y layers, and expands the design space to include fine structures and embedded multiphase components . Embedded 3D printing is known by many aliases, including “freeform 3D printing”, − “freeform reversible embedding (FRE)”, ,− “guest–host writing (GHost writing)”, “sacrificial writing into functional tissue (SWIFT)”, “suspended layer additive manufacturing (SLAM)”, , and “writing in granular gels”. , Notably, EIW allows incorporation of cells into bio-inks, which can improve cell adhesion compared to seeding cells on printed scaffolds. ,,− …”

Section: Introductionmentioning

confidence: 99%

Suppression of Filament Defects in Embedded 3D Printing

Friedrich

Gunther

Seppala

2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Embedded 3D printing enables the manufacture of soft, intricate structures. In the technique, a nozzle is embedded into a viscoelastic support bath and extrudes filaments or droplets. While embedded 3D printing expands the printable materials space to low-viscosity fluids, it also presents new challenges. Filament cross-sections can be tall and narrow, have sharp edges, and have rough surfaces. Filaments can also rupture or contract due to capillarity, harming print fidelity. Through digital image analysis of in situ videos of the printing process and images of filaments just after printing, we probe the effects of ink and support rheology, print speeds, and interfacial tension on defects in individual filaments. Using model materials, we determine that if both the ink and support are water-based, the local viscosity ratio near the nozzle controls the filament shape. If the ink is slightly more viscous than the support, a round, smooth filament is produced. If the ink is oil-based and the support is water-based, the capillary number, or the product of the ink speed and support viscosity divided by the interfacial tension, controls the filament shape. To suppress contraction and rupture, the capillary number should be high, even though this leads to trade-offs in roughness and roundness. Still, inks at nonzero interfacial tension can be advantageous, since they lead to much rounder and smoother filaments than inks at zero interfacial tension with equivalent viscosity ratios.

show abstract

Freeform 3D Bioprinting Involving Ink Gelation by Cascade Reaction of Oxidase and Peroxidase: A Feasibility Study Using Hyaluronic Acid-Based Ink

Cited by 8 publications

References 54 publications

Hyaluronic Acid as Bioink and Hydrogel Scaffolds for Tissue Engineering Applications

Hyaluronic Acid as Bioink and Hydrogel Scaffolds for Tissue Engineering Applications

Studies on PVP-Based Hydrogel Polymers as Dressing Materials with Prolonged Anticancer Drug Delivery Function

Suppression of Filament Defects in Embedded 3D Printing

Contact Info

Product

Resources

About