Free thiols in human spermatozoa: correlation with sperm DNA integrity

Zini, Armand; Kamal, Khaled; Phang, Donna

doi:10.1016/s0090-4295(01)00997-9

Cited by 42 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our fin dings con firm the results of Zi ni et al 25 however con tra dict the re sults of Kao et al, 7 Al kan et al, 26 and Ebisch et al 27 Zi ni et al de mons tra ted that sperm SH con tent cor re la ted posi ti vely with sperm DNA de na tu ra ti on and there were sig ni fi cantly hig her le vels of sperm DNA dena tu ra ti on and SH con tent the re we re in in fer ti le men. 25 Ka o et al sho wed that sperm with hig her le vels of oxi da ti ve da ma ge had lo wer amo unt of thi ols in se mi nal plas ma. 7 Al kan et al re por ted that se mi nal plas ma SH gro ups in in fer ti le pa ti ents were sig ni fi cantly lo wer than tho se in the con trol groups.…”

Section: Semensupporting

confidence: 91%

The Evaluation of Total Antioxidant and Oxidant Response in Seminal Plasma in Fertile and Infertile Men Using a Novel Automated Method

Yiğitbaşı¹,

Baskın²,

Çelik³

et al. 2011

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Oxi da ti ve stress has be en imp li ca ted in the pat ho ge ne sis of ma le in fer ti lity. In this study, we ai med to me a su re the an ti o xi dant ca pa city in se mi nal plas ma in fer ti le and infer ti le men using a re cently de ve lo ped au to ma ted me a su re ment met hod. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : The study gro up inc lu ded samp les of 55 ma les from in fer ti le co up les, and 16 he althy vo lun te ers ser ved as the con trol gro up. All samp les had nor mal sperm pa ra me ters. Se mi nal to tal an ti o xi dant res pon se (TAR) and to tal oxi dant sta tus (TOS) we re de ter mi ned using co lo ri met ric met hods. Oxi da ti ve stress in dex (OSI) was cal cu la ted as [(TOS/TAR) ×100]. Fre e sulphydryl (SH) gro ups of semen samp les we re me a su red spec trop ho to met ri cally. R Re e s su ul lt ts s: : Se mi nal TAR was sig ni fi cantly lo wer and se mi nal TOS, OSI and SH ac ti vi ti es we re sig ni fi cantly hig her in in fer ti le gro up as com pa red with fer ti le do nors. The re we re po si ti ve cor re la ti ons bet we en TAR and sperm pa ra me ters such as +4 mo ti lity (r= 0.386, p< 0.01), and morp ho logy (r= 0.383, p< 0.01), whe re as sperm con cen tra ti ons (r= -0.333, p< 0.01) cor re la ted ne ga ti vely with TAR. In ad di ti on, TOS and OSI were, negatively correlated with sperm pa ra me ters such as mo ti lity, +4 mo ti lity and morp ho logy (p< 0.01, for all). C Co on nc c l lu u s si i o on n: : In cre a sed oxi dant sta tus and dec re a sed an ti o xi dant res pon se and the cor re la ti on betwe en the se pa ra me ters and sperm func ti ons imp li ca ted that oxi da ti ve stress might play an im portant ro le in ma le in fer ti lity. We fo und dec re a sed to tal an ti o xi dant res pon se in in fer ti le pa ti ents using a simp le, ra pid and re li ab le au to ma ted co lo ri met ric as say, which may su i tab le for use in any ro u ti ne an dro logy la bo ra tory. K Ke ey y W Wo or rd ds s: : In fer ti lity; oxi da ti ve stress; se men Ö ÖZ ZE ET T A Am ma aç ç: : Er kek in fer ti li te si nin pa to ge ne zin de ok si da tif stres de dahil edil mek te dir. Bu ça lış -ma da, fer til ve in fer til er kek le rin se mi nal sı vı la rın da ki an ti ok si dan ka pa si te yi ye ni ge liş ti ril miş oto ma tik bir me tot la ölç me yi ve de ğer len me yi amaç la dık. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Ça lış ma gru bu infer til çift ler den 55 er kek has ta yı içe rir ken, sperm pa ra met re le ri nor mal olan 16 sağ lık lı gö nül lü de kon trol gru bu nu oluş tur du. Se men de ki to tal an ti ok si dan ya nıt (TAR) ve to tal ok si dan du rum (TOS) ko lo ri met rik yön tem ler le be lir len di. Ok si da tif stres in dek si (OSI); [(TOS/TAR) ×100] şek lin de hesap lan dı. Se men ör nek le rin de ki ser best sül fid ril grup la rı spek tro fo to met rik ola rak öl çül dü. B Bu ul l g gu u --l la ar r: : Er kek in fer ti li te si olan grup ta fer til gö nül lü ler le k...

show abstract

Section: Semensupporting

confidence: 91%

The Evaluation of Total Antioxidant and Oxidant Response in Seminal Plasma in Fertile and Infertile Men Using a Novel Automated Method

Yiğitbaşı¹,

Baskın²,

Çelik³

et al. 2011

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

show abstract

“…In addition to decreased P1 content, an effect on available reactive thiols could be due also to increased alkylation of protamine thiol groups; protamines in the testis are especially susceptible to alkylation, thus blocking normal disulphide bond formation (Sega and Owens, 1983), preventing proper chromatin condensation and altering sperm structure. Indeed, the thiol-disulphide status of protamines determines the AO fluorescence of spermatozoal nuclei, such that increased susceptibility to acidinduced DNA denaturation occurs if DNA-associated protamines are poor in disulphides (Kosower et al, 1992;Zini et al, 2001). Previously, we reported earlier male pronuclear formation in rat oocytes fertilized by CPA-treated males, as well as altered sperm chromatin decondensation, both in vitro and in denuded hamster oocytes (Qiu et al, 1995b;Harrouk et al, 2000).…”

Section: Discussionmentioning

confidence: 93%

“…Sperm chromatin structure Altered sperm chromatin structure has been attributed to changes in protamine content and the thiol-disulphide status of proteins (Kosower et al, 1992;Sailer et al, 1995;Love and Kenney, 1999;Zini et al, 2001;Aoki et al, 2005;Pina-Guzman et al, 2005). The SCSA w /AO DNA denaturation assay was used for a general assessment of the effects of CPA exposure on sperm chromatin structure.…”

Section: Resultsmentioning

confidence: 99%

Exposure of male rats to cyclophosphamide alters the chromatin structure and basic proteome in spermatozoa

Codrington¹,

Hales²,

Robaire

2007

Human Reproduction

View full text Add to dashboard Cite

BACKGROUND: The formation of mature sperm involves the expression of numerous proteins during spermiogenesis and the replacement of histones with protamines to package the genome. Exposure to cyclophosphamide (CPA), an anticancer alkylating agent, during spermiogenesis may disrupt chromatin condensation with adverse consequences to the offspring. METHODS: Adult male rats were given CPA in one of two schedules: (i) subchronic, 4 days-day 1 (100 mg kg 21) and days 2-4 (50 mg kg 21 per day) or (ii) chronic-daily (6.0 mg kg 21 per day). Animals were euthanized on days 14, 21 or 28. RESULTS: The effects of CPA on epididymal sperm chromatin structure were germ-cell-phase specific; mid-spermiogenic spermatids were most sensitive. The acridine orange DNA denaturation assay showed significant increases in susceptibility to denaturation (P < 0.01). Chromatin packaging assessment revealed 1,4-dithiothreitol-dependent chromomycin A3 DNA binding and less condensed, protaminedeficient sperm; the total thiol (P < 0.001) and protamine contents (P < 0.01), measured using monobromobimane and the HUP1N protamine 1 antibody, respectively, were reduced. The sperm basic proteome was also altered; proteins that were identified are involved in events during spermiogenesis and fertilization. CONCLUSIONS: Paternal exposure to CPA alters sperm chromatin structure, as well as the composition of sperm head basic proteins. We speculate that these changes underlie effects on fertilization and embryo development.

show abstract

“…Condensation of spermatid chromatin is accompanied by replacement of histones with protamines, which are basic nuclear proteins with a high Cys content, as well as by subsequent oxidation of protamine thiol groups to form disulfide linkages that result in protein crosslinking (32,34). The chromatin of sperm is highly compacted to protect the DNA from deleterious environments with insufficient oxidation of protamine thiol groups being associated with damage to sperm DNA (35)(36)(37). The reduction of sperm disulfides followed by decondensation of sperm chromatin in readiness for fertilization is accomplished in oocytes, which contain high levels of glutathione (38).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Alternatively Transcribed Form of Peroxiredoxin IV Gene That Is Specifically Expressed in Spermatids of Postpubertal Mouse Testis

Yim

Kim

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

Free thiols in human spermatozoa: correlation with sperm DNA integrity

Cited by 42 publications

References 23 publications

The Evaluation of Total Antioxidant and Oxidant Response in Seminal Plasma in Fertile and Infertile Men Using a Novel Automated Method

The Evaluation of Total Antioxidant and Oxidant Response in Seminal Plasma in Fertile and Infertile Men Using a Novel Automated Method

Exposure of male rats to cyclophosphamide alters the chromatin structure and basic proteome in spermatozoa

Identification and Characterization of Alternatively Transcribed Form of Peroxiredoxin IV Gene That Is Specifically Expressed in Spermatids of Postpubertal Mouse Testis

Contact Info

Product

Resources

About