“…Four other minor Δ 5 sterols occurred in all five species: 24-methylcholesterol (24α/24R = campesterol 36 , 24β/24S = 22-dihydrobrassicasterol 38 ), 24-ethylcholesta-5,22 E -dienol (24α/24S = stigmasterol 39 , 24β/24R = poriferasterol 40 ), 24-methylcholesta-5,22 E -dienol (24α/24S = crinosterol 41 , 24β/24R = brassicasterol 42 ), and 24-ethylidenecholesterol, which was tentatively assigned by the authors as the Δ 24(28)Z isomer = isofucosterol 44 . Among the TMS-derivatized sterols of Acetabularia caliculus , 24-ethylcholest-7-enol 46 / 47 was identified ( Figure 4 , Table 1 ) [ 52 ]. Prior studies had also identified cholesterol and 24-methylenecholesterol 45 in cultures of Acetabularia mediterranea , suggested by the authors to potentially be a result of differences in algal cultivation.…”