Free-standing polysaccharide composite films: Improved preparation and physical properties

Hashizume, Makoto; Ohashi, Masataka; Kobayashi, Hironobu; Tsuji, Yuna; Iijima, Kazutoshi

doi:10.1016/j.colsurfa.2015.07.033

Cited by 18 publications

(24 citation statements)

References 51 publications

(57 reference statements)

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“…CS/CHI films with a thickness of ≈100 µm under dry conditions were prepared according to our previous study . The film was yellow, transparent, and free‐standing and became soft and flexible after becoming swollen in solution.…”

Section: Resultsmentioning

confidence: 99%

“…Polysaccharide composite films made of CS and CHI were prepared according to our previous study . Briefly, 2 wt% aqueous solutions of CS were added drop wise to 1 wt% CHI solution in 1 wt% aqueous acetic acid until the formation of gel‐like PICs was completed.…”

Section: Methodsmentioning

confidence: 99%

“…The swelling behaviors of the films were evaluated according to previously described methods . Films (1 cm × 1 cm) were immersed in ultrapure water and other buffer solutions and incubated at room temperature for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…We developed polysaccharide composite films made of anionic polysaccharides such as chondroitin sulfate C (CS), hyaluronic acid, and heparin, and the cationic polysaccharide chitosan using the hot press technique . In this method, free‐standing transparent films with thicknesses of several tenths of micrometers were obtained in less than 30 min by hot pressing PIC gels . We also investigated the potential use of composite films made of CS and CHI (hereafter denoted as CS/CHI films) as drug carriers .…”

Section: Introductionmentioning

confidence: 99%

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Kinetic Analysis of Molecular Permeabilities of Free‐Standing Polysaccharide Composite Films

Iijima

Kimura

Sato

et al. 2016

Macro Chemistry & Physics

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This study is performed in order to assess the molecular permeabilities of composite films of chondroitin sulfate C (CS) and chitosan (CHI) prepared by the hot press technique. Permeation behaviors are evaluated using horizontal diffusion cells and four substrates with different charges: methylene blue (MB), orange II (OR), l‐tryptophan (Trp), and 4‐methylumbelliferyl β‐d‐galactopyranoside (MUG). Permeability coefficients, diffusion coefficients, and partition coefficients are calculated from permeation curves. When MB and OR are used as the permeants, permeability coefficients in solutions with higher pH or ionic strength are greater than in those with lower pH or ionic strength. Permeability coefficients at pH 5.9 at which Trp carries zero net charge are as follows: Trp > MB ≈ MUG > OR. The diffusion coefficients and partition coefficients obtained indicate that CS/CHI films permeate these molecules based on a partition mechanism at lower pH and a pore model at higher pH. These characteristics appear to reflect the unique microenvironment of films consisting of polyion complexes of oppositely charged polysaccharides.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kinetic Analysis of Molecular Permeabilities of Free‐Standing Polysaccharide Composite Films

Iijima

Kimura

Sato

et al. 2016

Macro Chemistry & Physics

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“…Uniform, smooth films cannot be obtained by casting gel-like PICs [16]. Therefore, other techniques, such as layer-by-layer (LbL) assembly [17,18] and a hot-press technique [19,20], have been developed for fabrication of uniform and smooth films made of polysaccharide PICs. In the LbL assembly technique, substrates are immersed alternately in cationic and anionic aqueous solutions of polysaccharides and result in the formation of polysaccharide composite films [17,18].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles

Yamazaki

Iijima

2020

Polymers

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Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures. This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components. Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick. These films were homogenous and water insoluble. It was revealed that the component anionic polysaccharides affected the film’s properties such as the swelling behavior and mechanical characteristics. Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined. Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin. However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44. These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films. It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides. These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.

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Media-Responsive Swelling and Material Release Properties of Polysaccharide Composite Films

Hashizume

Iijima

2016

Stimuli-Responsive Interfaces

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Free-standing polysaccharide composite films: Improved preparation and physical properties

Cited by 18 publications

References 51 publications

Kinetic Analysis of Molecular Permeabilities of Free‐Standing Polysaccharide Composite Films

Kinetic Analysis of Molecular Permeabilities of Free‐Standing Polysaccharide Composite Films

Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles

Media-Responsive Swelling and Material Release Properties of Polysaccharide Composite Films

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