Free‐Standing Biodegradable Poly(lactic acid) Nanosheet for Sealing Operations in Surgery

Okamura, Yosuke; Kabata, Koki; Kinoshita, Manabu; Saitoh, Daizoh; Takeoka, Shinji

doi:10.1002/adma.200901035

Cited by 157 publications

(222 citation statements)

References 29 publications

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“…In the case of the PLLA nanosheet-coated sample, the C1s peak was split into three subpeaks, representing the C-H signal of the methyl group at 284.0 eV, the C-O signal at 285.9 eV, and the C ¼ OO signal of the carboxylic group at 288.4 eV. These subpeaks were consistent with the characteristic molecular structures of PLLA, 15,29 supporting the presence of PLLA in the surface layer. From these results, we concluded that the modified PDMS surface was almost completely covered by the fragmented PLLA nanosheets.…”

Section: A Characterization Of the Modified Surfacessupporting

confidence: 58%

“…15 In this study, we examined the long-term durability of the nanosheet-coated microchannel under dynamic flow conditions. As shown in Figure 4(b), the coverage ratio remained nearly unchanged, even after undergoing quite high shear stresses of 10.7 dyn/cm 2 and 21.3 dyn/cm 2 for an extended duration.…”

Section: B Microchannel Coatingmentioning

confidence: 99%

“…Additionally, poly(L-lactic acid) (PLLA), another important biocompatible and biodegradable polymer, has been fabricated as an ultrathin film (often referred to as a nanosheet) for application as a sealing material in surgery. 15 This freestanding nanosheet, which has a thickness of less than 100 nm, has been shown to have high potential to adhere to glass, plastic, skin, and organs without the use of adhesive reagents. Furthermore, fragmented nanosheets, which refers to the fragments of nanosheet fabricated by homogenization of the original nanosheet, can also be used to coat both broad and irregular surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Surface modification on polydimethylsiloxane-based microchannels with fragmented poly(l-lactic acid) nanosheets

2015

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Surface modification is a critical issue in various applications of polydimethylsiloxane (PDMS)-based microfluidic devices. Here, we describe a novel method through which PDMS-based microchannels were successfully modified with fragmented poly(L-lactic acid) (PLLA) nanosheets through a simple patchwork technique that exploited the high level of adhesiveness of PLLA nanosheets. Compared with other surface modification methods, our method required neither complicated chemical modifications nor the use of organic solvents that tend to cause PDMS swelling. The experimental results indicated that the modified PDMS exhibited excellent capacity for preventing the adhesion and activation of platelets. This simple yet efficient method can be used to fabricate the special PDMS microfluidic devices for biological, medical, and even hematological purposes. V C 2015 AIP Publishing LLC.

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Section: A Characterization Of the Modified Surfacessupporting

confidence: 58%

Section: B Microchannel Coatingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Surface modification on polydimethylsiloxane-based microchannels with fragmented poly(l-lactic acid) nanosheets

2015

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“…Previous studies from the laboratory of Shinji Takeoka demonstrate excellent sealing efficacy of a poly-L-lactic acid (PLLA) nanofilm for gastric incisions as a novel wound dressing material that did not require adhesive agents, induce scars or adhere to surrounding tissues (7). This same group also demonstrated the efficacy of PLLA sheets as a method for fixing a polypropylene mesh in the peritoneal cavity without inducing visceral adhesions during open surgery (8).…”

Section: Introductionmentioning

confidence: 66%

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

et al. 2014

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Preterm premature rupture of membranes (PPROM) causes 40% of all preterm births, affecting 150,000 women each year in the United States. Prenatal diagnostic procedures and surgical interventions increase incidence of adverse events, leading to iatrogenic membrane rupture after a fetoscopic procedure in 45% of cases. We propose an ultrathin, self-adherent, poly-L-lactic acid patch ("nanofilm") as a reparative wound closure after endoscopic/fetoscopic procedures. These nanofilms are compatible with application in wet conditions and with minimally invasive instrumentation.Ex vivo studies to evaluate the nanofilm were conducted using human chorion-amnion (CA) membranes. A custom-built inflation device was used for mechanical characterization of CA membranes and for assessment of nanofilm adhesion and sealing of membrane defects up to 3 mm in size. These ex vivo tests demonstrated the ability of the nanofilm to seal human CA defects ranging in size from 1 to 3 mm in diameter. In vivo survival studies were conducted in 25 mid-gestational rabbits, defects were created by perforating the uterus and the CA membranes and subsequently using the nanofilm to seal these wounds. These in vivo studies confirmed the successful sealing of defects smaller than 3 mm observed ex vivo.Histological analysis of whole harvested uteri 7 days after surgery showed intact uterine walls in 59% of the nanofilm repaired fetuses, along with increased uterine size and intrauterine development in 63% of the cases. In summary, we have developed an ultrathin, self-adhesive nanofilm for repair of uterine membrane defects.

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“…[5][6][7][8] They are also used for fundamental studies exploring the behavior of polymers under confinement. [9][10][11][12][13] A free-standing film is one that is supported on a non-continuous substrate such as an annular ring or hoop as opposed to a silicon wafer or glass slide.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Large-area Free-standing Ultrathin Polymer Films

Stadermann

Baxamusa

Aracne‐Ruddle

et al. 2015

JoVE

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This procedure describes a method for the fabrication of large-area and ultrathin free-standing polymer films. Typically, ultrathin films are prepared using either sacrificial layers, which may damage the film or affect its mechanical properties, or they are made on freshly cleaved mica, a substrate that is difficult to scale. Further, the size of ultrathin film is typically limited to a few square millimeters. In this method, we modify a surface with a polyelectrolyte that alters the strength of adhesion between polymer and deposition substrate. The polyelectrolyte can be shown to remain on the wafer using spectroscopy, and a treated wafer can be used to produce multiple films, indicating that at best minimal amounts of the polyelectrolyte are added to the film. The process has thus far been shown to be limited in scalability only by the size of the coating equipment, and is expected to be readily scalable to industrial processes. In this study, the protocol for making the solutions, preparing the deposition surface, and producing the films is described. Video LinkThe video component of this article can be found at

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Free‐Standing Biodegradable Poly(lactic acid) Nanosheet for Sealing Operations in Surgery

Cited by 157 publications

References 29 publications

Surface modification on polydimethylsiloxane-based microchannels with fragmented poly(l-lactic acid) nanosheets

Surface modification on polydimethylsiloxane-based microchannels with fragmented poly(l-lactic acid) nanosheets

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

Fabrication of Large-area Free-standing Ultrathin Polymer Films

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