Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Benchikh, Fatima; Benabdallah, Hassiba; Amira, Hind; Amira, Islam; Mamache, Walid; Amira, Smain

doi:10.24925/turjaf.v10i6.1089-1094.5062

Cited by 7 publications

(4 citation statements)

References 22 publications

(23 reference statements)

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“…The ABTS and FRAP scavenging activity of MMEx was reflected by IC 50 values of 22.83 ± 0.56 and 47.09 ± 4.41 µg/mL, respectively. A similar trend was observed for the ABTS •+ removal capacity of MMEx, where the IC 50 was 13.6 μg.mLl −1 [ 62 ]. In another study, the authors reported that myrtle extract effectively scavenged ABTS •+ , with an IC 50 of 4.8 µg.mL −1 [ 45 ].…”

Section: Discussionsupporting

confidence: 77%

Chemical Composition Antioxidant and Anti-Inflammatory Activities of Myrtus communis L. Leaf Extract: Forecasting ADMET Profiling and Anti-Inflammatory Targets Using Molecular Docking Tools

Belahcene,

Kebsa,

Akingbade

et al. 2024

Molecules

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Compounds derived from natural sources continue to serve as chemical scaffolds for designing prophylactic/therapeutic options for human healthcare. In this study, we aimed to systematically unravel the chemical profile and antioxidant and anti-inflammatory activities of myrtle methanolic extract (MMEx) using in vitro, in vivo, and in silico approaches. High levels of TPC (415.85 ± 15.52 mg GAE/g) and TFC (285.80 ± 1.64 mg QE/g) were observed. Mass spectrophotometry (GC-MS) analysis revealed the presence of 1,8-cineole (33.80%), α-pinene (10.06%), linalool (4.83%), p-dimethylaminobenzophenone (4.21%), thunbergol (4%), terpineol (3.60%), cis-geranyl acetate (3.25%), and totarol (3.30%) as major compounds. MMEx induced pronounced dose-dependent inhibition in all assays, and the best antioxidant activity was found with H2O2, with an IC50 of 17.81 ± 3.67 µg.mL−1. MMEx showed a good anti-inflammatory effect in vivo by limiting the development of carrageenan-induced paw edema. The pharmacokinetic profiles of the active molecules were determined using the SwissADME website, followed by virtual screening against anti-inflammatory targets including phospholipase A2 (PLA-2), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and NF-κB. A pharmacokinetic study revealed that the molecules have good absorption, distribution, and metabolism profiles, with negative organ toxicity. Among the compounds identified by GC-MS analysis, pinostrobin chalcone, cinnamyl cinnamate, hedycaryol, totarol, and p-dimethylaminobenzophenone were observed to have good binding scores, thus appreciable anti-inflammatory potential. Our study reveals that MMEx from Algerian Myrtus communis L. can be considered to be a promising candidate for alleviating many health complaints associated with oxidative stress and inflammation.

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Section: Discussionsupporting

confidence: 77%

Chemical Composition Antioxidant and Anti-Inflammatory Activities of Myrtus communis L. Leaf Extract: Forecasting ADMET Profiling and Anti-Inflammatory Targets Using Molecular Docking Tools

Belahcene,

Kebsa,

Akingbade

et al. 2024

Molecules

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“…The in‐vitro antioxidant activity of aqueous extract of N. sativa was done by previously explained methods with minor modification. DPPH ● scavenging activity (More & Makola, 2020; Shrivastava et al, 2021), nitric oxide (NO ● ) scavenging activity (Kamble et al, 2020), hydrogen peroxide (H 2 O 2 ) scavenging activity (Kamble et al, 2020), Hydroxyl (OH ● ) radical scavenging activity (Ujowundu, 2017), Metal chelating activity (Benchikh et al, 2022), Ferric reducing power (FRAP) activity (Kamble et al, 2020), total antioxidant activity (Kamble et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

LC‐MS based metabolite profiling, in‐vitro antioxidant and in‐vivo antihyperlipidemic activity of Nigella sativa extract

Shrivastava

Shrestha

Pokhrel

et al. 2023

eFood

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The aim of this study was to identify the bioactive phytoconstituents present in the aqueous extract of Nigella sativa and also, to evaluate the antioxidant and antihyperlipidemic activity in Wistar rats. The LC‐MS/MS analysis was assessed for the determination of different bioactive compounds present in N. sativa extract. Total phenolic and flavonoid content were determined by using validated Folin‐Ciocalteu and Aluminum chloride colorimetric methods, respectively. The in‐vitro antioxidant and in‐vivo antihyperlipidemic activity in Wistar rats were also evaluated. Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, phenols, glycosides, and amino acids in the aqueous extract. The bioactive compounds of the aqueous extract were identified through LC‐MS/MS analysis. The in‐vitro antioxidant activity of N. sativa showed the highest free radical scavenging capacity in DPPH, H2O2, and OH radical scavenging assays with IC50 values 11.916 ± 2.828, 30.294 ± 13.790, and 12.048 ± 2.828 µg/mL, respectively. Evaluation of antihyperlipidemic activity of extract in Wistar rats showed that a high dose (800 mg/kg) of extract significantly decreased total cholesterol (TC) 71.76 ± 6.91 mg/dL, TG 83.6 ± 8.09 mg/dL, low‐density lipoproteins (LDL‐c) 33.86 ± 6.05 mg/dL, very low‐density lipoproteins (VLDL‐c) 16.72 ± 1.61 mg/dL level in blood. However, the HDL‐C level was significantly improved (21.18 ± 1.80 mg/dL) as compared to HFD‐induced control rats (11.76 ± 1.14 mg/dL) after 28 days of treatment. Also, at the same dose, animal body weight was also decreased to 162.6 ± 16.40 g compared with control 184.4 ± 10.24 g. The aqueous extract of N. sativa was found to be an effective natural source of antioxidant and hypolipidemic agents. This activity was attributed to the presence of diverse bioactive compounds in it.

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“…In biological systems, iron metal by reaction with superoxide anions (Haber-Weiss process) or hydrogen peroxide (Fenton reaction) leads to the production of highly reactive hydroxyl radicals. Metal chelating activity refers to the ability of plant extracts to bind or chelate metal ions, preventing them from participating in oxidative reactions and reducing their potential to generate harmful free radicals [19]. In the current method, presence of metal chelators in plant extracts by removing ferrous ions disrupted the formation of the Ferrozine-Fe 2+ complex, causing a dose-dependent decrease in absorbance at 562 nm.…”

Section: Metal Chelating Activitymentioning

confidence: 99%

Screening of Salvadora persica leaves for antioxidant properties and total phenolic content

Saini¹

2023

World J. Bio. Pharm. Health Sci.

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The objective of this research was to investigate the antioxidant properties of Salvadora persica leaf extracts using various in vitro assays and compare them with the total phenolic content. Petroleum ether, benzene, chloroform, methanol, and aqueous extracts were assessed for their ability to scavenge free radicals such as DPPH, superoxide, and hydroxyl radicals along with metal chelating capacity and reducing power. Estimation of phenolic content was done using the Folin-ciocalteu reagent and expressed as mg/g Gallic acid equivalent. The results demonstrated that all the solvents exhibited significant concentration-dependent free radical scavenging activity. Among the five extracts, the methanolic extract displayed the highest antioxidant activity and contained 52.64 mg/g Gallic acid equivalents (GAE) of phenolic content. The study identified significant correlation between the total phenolic content and the antioxidant assays suggesting role of phenolic compounds to the antioxidant activity of S. persica extracts. These findings suggest that the methanolic extract of S. persica is rich in phenolic compounds, which may account for its potent antioxidant activity. Consequently, these results emphasize the potential of S. persica as a valuable source of natural antioxidants and provide scientific support for its use in treating various health conditions.

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Free Radical Scavenging, Metal chelating and Antiperoxidative Activities of M. communis Berries Methanol extract and its Fractions

Cited by 7 publications

References 22 publications

Chemical Composition Antioxidant and Anti-Inflammatory Activities of Myrtus communis L. Leaf Extract: Forecasting ADMET Profiling and Anti-Inflammatory Targets Using Molecular Docking Tools

Chemical Composition Antioxidant and Anti-Inflammatory Activities of Myrtus communis L. Leaf Extract: Forecasting ADMET Profiling and Anti-Inflammatory Targets Using Molecular Docking Tools

LC‐MS based metabolite profiling, in‐vitro antioxidant and in‐vivo antihyperlipidemic activity of Nigella sativa extract

Screening of Salvadora persica leaves for antioxidant properties and total phenolic content

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