Free Radical Scavenging and Hepatoprotective Actions of the Medicinal Herb, Crassocephalum crepidioides from the Okinawa Islands

Aniya, Yoko; Koyama, Tomoyuki; Miyagi, Chika; Miyahira, Manami; Inomata, Chiho; Kinoshita, Shizuka; Ichiba, Toshio

doi:10.1248/bpb.28.19

Cited by 110 publications

(94 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In another setting, incubation with C. crepidioides for 3 h at 15.6 μg/ml induced iNOS mRNA expression in RAW264.7 cells (Figure 4B). The main antioxidant isolated from C. crepidioides is isochlorogenic acid [3]. Isochlorogenic acid stimulated iNOS expression in a manner similar to that by C. crepidioides (Figure 4C and D).…”

Section: Resultsmentioning

confidence: 93%

“…Compounds with antioxidant activities have been isolated previously from C. crepidioides [3], including isochlorogenic acid and the flavonoids quercetin and kaempferol. However, the latter two have anti-inflammatory properties and are known to inhibit LPS-induced iNOS expression and NO production [32,33].…”

Section: Discussionmentioning

confidence: 99%

“…The extract obtained was used as an original extract, and dissolved with pure water when necessary. Isochlorogenic acid was purified using the procedure described previously with some modifications [3]. The extract dissolved in pure water was applied to a HP-20 (Mitsubishi Chemical, Tokyo, Japan) column eluting water and increasing amount of methanol (MeOH) to yield 70% MeOH fraction.…”

Section: Methodsmentioning

confidence: 99%

“…Compounds with antimalarial activity and strong antimutagenicity had been isolated previously from C. crepidioides [1,2]. Other preparations from C. crepidioides have been described to have antioxidant and hepatoprotective properties [3]. However, the anticancer activity of C. crepidioides has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

Tomimori

Nakama

Kimura

et al. 2012

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundCrassocephalum crepidioides, a plant distributed in Okinawa Islands, is known in folk medicine; however, its anticancer activity has not been investigated. The aim of this study was to determine the in vitro and in vivo antitumor activities of C. crepidioides on murine Sarcoma 180 (S-180) and related molecular mechanisms.MethodsThe antitumor effect of C. crepidioides was evaluated in S-180-cell-bearing mice. Cell growth was assessed using a colorimetric assay. Nitrite and nitrate levels were measured by colorimetry. The expression levels of inducible NO synthase (iNOS) in murine RAW264.7 macrophages was assessed by reverse transcriptase-polymerase chain reaction. Activation of iNOS promoter was detected by reporter gene. Activation of nuclear factor-κB (NF-κB) was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling was analyzed using inhibitors of NF-κB and dominant-negative mutants, and Western blot analysis.ResultsC. crepidioides extract delayed tumor growth in S-180-bearing mice. However, it did not inhibit S-180 cell growth in vitro. Supernatant of cultured C. crepidioides-stimulated RAW264.7 macrophages was cytotoxic to S-180 cells. This cytotoxicity was associated with nitric oxide (NO) production. NF-κB signaling pathway was crucial for the transcriptional activation of iNOS gene. Isochlorogenic acid, a component of C. crepidioides, induced NF-κB activation and iNOS expression.ConclusionsThe results highlight the oncolytic and immunopotentiation properties of C. crepidioides mediated through NF-κB-induced release of NO from macrophages.

show abstract

Section: Resultsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

Tomimori

Nakama

Kimura

et al. 2012

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Various dose combinations of LPS and D-GalN were used to produce a sub-lethal liver failure, which is relevant to clinical scenarios. In the present study, we used a low dose of 0.5 lg/kg of LPS combined with 800 mg/kg D-GalN as previously reported (Aniya et al, 2005). This regimen induces changes in the permeability of the plasma membrane and leakage of liver enzymes from the cells (Mitra et al, 2000).…”

Section: Discussionmentioning

confidence: 94%

Hepatoprotective effect ofCommiphora myrrhaagainstd-GalN/LPS-induced hepatic injury in a rat model through attenuation of pro inflammatory cytokines and related genes

Ahmad

Raish

Ganaie

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

(2015) Hepatoprotective effect of Commiphoramyrrha against d-GalN/LPS-induced hepatic injury in a rat model through attenuation of pro inflammatory cytokines and related genes, Pharmaceutical Biology, 53:12, 1759-1767, DOI: 10.3109/13880209.2015 Results: The administration of D-GalN/LPS increased plasma aminotransferases (174.47 ± 4.5761 and 260.96 ± 1.9839 mkat/l) and total bilirubin levels (1.012 ± 0.0288 mg/dl), which were attenuated by C. myrrha treatment. Hepatic lipid peroxidation activity and nitric oxide content also increased, while the antioxidant activity measured by GSH (0.76 nmol/g protein), SOD (81.91 U/mg protein), and CAT (15.78 U/mg protein) was reduced. Commiphora myrrha provided significant restoration of GSH (0.815 nmol/gm protein), SOD (140.57 U/mg protein), and CAT (27.02 U/mg protein) levels. Furthermore, the acute phase response elicited by D-GalN/ LPS administration enhanced mRNA expressions of TNF-a, IL-6, IL-10, iNOS-2, and HO-1, which were ameliorated by C. myrrha treatment. Discussion and conclusion: These findings indicate that C. myrrha considerably reduces the oxidative stress of D-GalN/LPS-induced hepatic injury via multiple pathways including adown regulation of inflammatory mediators and cytokines. Such a property might be sufficient to combat cellular damage caused by various conditions that resemble fulminant hepatitis and could be of a potential clinical application.

show abstract

Nutritional and functional properties of cookies made using down‐graded lentil – A candidate for novel food production and crop utilization

et al. 2019

View full text Add to dashboard Cite

show abstract

Free Radical Scavenging and Hepatoprotective Actions of the Medicinal Herb, Crassocephalum crepidioides from the Okinawa Islands

Cited by 110 publications

References 23 publications

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides

Hepatoprotective effect ofCommiphora myrrhaagainstd-GalN/LPS-induced hepatic injury in a rat model through attenuation of pro inflammatory cytokines and related genes

Nutritional and functional properties of cookies made using down‐graded lentil – A candidate for novel food production and crop utilization

Contact Info

Product

Resources

About