Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi

Gao, Zhonghong; Huang, Kaixun; Yang, Xiangliang; Xu, Huibi

doi:10.1016/s0304-4165(99)00152-x

Cited by 554 publications

(331 citation statements)

References 19 publications

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“…Some antioxidant properties have been described for baicalein and NDGA that are independent of the LOX inhibition (48,49). However, we have previously described how the broad spectrum radical scavenger, ascorbic acid (50), prevents cell death in this model when added to the culture 30 min before or 2 h after NO treatment, but not when added 6 or 10 h after NO treatment (25).…”

Section: A-d)mentioning

confidence: 98%

Nitric Oxide Triggers the Toxicity due to Glutathione Depletion in Midbrain Cultures through 12-Lipoxygenase

Canals¹,

Casarejos

Bernardo

et al. 2003

Journal of Biological Chemistry

100

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Glutathione (GSH) depletion is the earliest biochemical alteration shown to date in brains ofHere we demonstrate that arachidonic acid (AA) metabolism through the 12-lipoxygenase (12-LOX) pathway is also central for this GSH-NO interaction. LOX inhibitors (nordihydroguaiaretic acid and baicalein), but not cyclooxygenase (indomethacin) or epoxygenase (clotrimazole) ones, prevent cell death in the culture, even when added 10 h after NO treatment. Furthermore, the addition of AA to GSH-depleted cultures precipitates a cell death process that is indistinguishable from that initiated by NO in its morphology, time course, and 12-LOX, GC, and PKG dependence. The first AA metabolite through the 12-LOX enzyme, 12-hydroperoxyeicosatetraenoic acid, induces cell death in the culture, and its toxicity is greatly enhanced by GSH depletion. In addition we show that if GSH synthesis inhibition persists for up to 4 days without any additional treatment, it will induce a cell death process that also depends on 12-LOX, GC, and PKG activation. In this study, therefore, we show that the signaling pathway AA/12-LOX/12-HPETE/GC/ PKG may be important in several pathologies in which GSH decrease has been documented, such as Parkinson's disease. The potentiating effect of NO over such a signaling pathway may be of relevance as part of the cascade of events leading to and sustaining nerve cell death.

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Section: A-d)mentioning

confidence: 98%

Nitric Oxide Triggers the Toxicity due to Glutathione Depletion in Midbrain Cultures through 12-Lipoxygenase

Canals¹,

Casarejos

Bernardo

et al. 2003

Journal of Biological Chemistry

100

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“…Flavonoids such as baicalin, wogonoside, wogonin, and oroxylin A are the main constituents in the radix of this plant, and more than 30 flavonoids have been identified up till now [3]. The extract of S. baicalensis has been reported to possess a variety of biological effects including anti-oxidant, anti-inflammatory, anti-bacterial, anti-tumor, and anti-diabetic activities [4][5][6][7][8]. Baicalin was shown to possess anti-diabetic activities in type 2 diabetic Goto-Kakizaki rats [9], and baicalin, baicalein, and wogonin were found to be α-glucosidase inhibitors, which are considered to be potential leading compounds for development of anti-diabetes drug [10].…”

Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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“…Os ácidos graxos têm importância energética e constituem componentes fundamentais de rotas metabólicas específicas. O ácido linoléico e, particularmente, seu isômero, ácido linoléico conjugado (CLA), são conhecidos por seus efeitos benéficos à saúde humana como efeito hipocolesterolêmico, estimulante do sistema imunológico, anticarcinogênico, sendo principalmente, antioxidante (SANHUEZA et al, 2002 (GAO et al, 1999). Segundo BAKKALBASI et al (2005), compostos fenólicos, particularmente, o flavan-3-ol tem comprovada capacidade de seqüestro de radicais livres, o que estimula a pesquisa e utilização desses compostos.…”

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Atividade antibacteriana, antioxidante e tanante de subprodutos da uva

et al. 2009

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Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi

Cited by 554 publications

References 19 publications

Nitric Oxide Triggers the Toxicity due to Glutathione Depletion in Midbrain Cultures through 12-Lipoxygenase

Nitric Oxide Triggers the Toxicity due to Glutathione Depletion in Midbrain Cultures through 12-Lipoxygenase

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

Atividade antibacteriana, antioxidante e tanante de subprodutos da uva

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