Free radical-scavenging activity of Taiwanese native plants

Hou, Wen Chi; Lin, Rong Dih; Cheng, Kur Ta; Hung, Yun-Jie; Cho, C.-H.; Chen, C.-H.; Hwang, Shu-Yuen; Lee, M.-H.

doi:10.1078/094471103321659898

Cited by 136 publications

(72 citation statements)

References 22 publications

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“…The antioxidant activity was evaluated using a DPPH radical-scavenging method that has been widely used to measure the radical-scavenging ability of plant extracts and their constituents, and the IC 50 value represents the concentration of sample required to scavenge 50% of DPPH free radicals. 20,21) The CH-ext exhibited potent radical-scavenging activity (IC 50 0.2 mg/ml) as shown in Table 4. As shown in Table 2, the radical-scavenging activity of neohesperidin (IC 50 0.6 mM) was more potent than that of hesperidin (IC 50 3.2 mM), whereas naringin (IC 50 Ͼ4 mM) and narirutin (IC 50 Ͼ4 mM) were inactive.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Citrus hassaku Extract and Its Flavanone Glycosides on Melanogenesis

Itoh

Hirata

Masuda

et al. 2009

Biological & Pharmaceutical Bulletin

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Melanogenesis stimulated by UV irradiation occurs in plants, microorganisms, and mammalian cells by an enzymatic oxidation process starting with L-tyrosine. Various ingredients for skin-whitening cosmetics are developing to reduce melanogenesis. Tyrosinase catalyzes the oxidation of L-tyrosine to 3,4-dihydroxyphenyl-L-alanine (L-DOPA), followed by the oxidation of L-DOPA to dopaquinone, and oxidative polymerization of several dopaquinone derivatives produces melanin. Thus the tyrosinase inhibitor is one of the candidates for reduction of melanogenesis.1) On the other hand, it has been reported that superoxide dismutase (SOD) is one of the key factors that reduce melanin production caused by UV irradiation.2) Therefore tyrosinase inhibitors with SOD-like activity and/or antioxidant activity may be useful ingredients in the field of skin-whitening cosmetics. During our screening program to find a potential tyrosinase inhibitor from natural resources, we reported several crude drugs, such as Glebnia littoralis F. SCHMIDT, Prunus zippeliana M., Myrica rubra S. et ZUCC., and Arctostaphylos uva-ursi L. SPRENGEL, some of which have been applied to cosmetic beauty preparations. 1,[3][4][5] Recently, the tyrosinase inhibitory activities of the peel of Citrus fruit (Citrus unshiu Markovich) and its flavonoids, such as nobiletin, have been reported. 6,7) As a part of our continuous studies on the biological activities of Citrus species, [8][9][10][11][12] we found that a 50% ethanolic extract (CH-ext) obtained from the unripe fruit of Citrus hassaku HORT ex T. TANAKA, which was collected by thinning out in July, exhibited potent mushroom tyrosinase inhibitory activity. Thinning out the unripe fruit of C. hassaku in July is important for a rich harvest of ripe fruit in December. Thus this study was undertaken to examine whether the unripe fruit of C. hassaku collected in July by thinning can be utilized as a plant resource for skin-whitening cosmetic agents, because, to the best of our knowledge, there is no report on the tyrosinase inhibitory activity of C. hassaku. First, to identify the active component, we carried out activity-guided fractionation of the CH-ext using tyrosinase inhibitory assay. For antioxidant activity, SOD-like and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the CH-ext and its flavanone glycosides were also studied. Second, according to the method of Imokawa, 13) we examined the effects of the CH-ext on melanogenesis using cultured murine B16 melanoma cells after exposure to glucosamine. Third, we examined the in vivo preventive effects of the CH-ext against UVB-induced pigmentation of dorsal skin in brownish guinea pigs. 14) MATERIALS AND METHODSReagents Hesperidin, naringin, and neohesperidin were purchased from Sigma-Aldrich Japan (Tokyo, Japan). Narirutin was isolated from fruit of C. unshiu.10) Other chemical and biochemical reagents were of reagent grade and were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and/or Nacalai Tesque, Inc. (Kyoto, Japan)...

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Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Citrus hassaku Extract and Its Flavanone Glycosides on Melanogenesis

Itoh

Hirata

Masuda

et al. 2009

Biological & Pharmaceutical Bulletin

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“…A quantificação espectrométrica de compostos fenólicos é realizada por meio de uma variedade de técnicas, todavia, a que utiliza o reagente de Folin-Ciocalteu figura entre as mais extensivamente utilizadas 2,4,7,9,10 . O reagente consiste de mistura dos ácidos fosfomolibídico e fosfotunguístico, no qual o molibdênio e o tungstênio encontram-se no estado de oxidação 6 + porém, em presença de certos agentes redutores, como os compostos fenólicos, formam-se os chamados molibdênio azul e tungstênio azul, nos quais a média do estado de oxidação dos metais está entre 5 e 6 e cuja coloração permite a determinação da concentração das substâncias redutoras, que não necessariamente precisam ter natureza fenólica 4,11 .…”

Section: Determinação De Fenóis E Polifenóisunclassified

Fenóis totais e atividade antioxidante de cinco plantas medicinais

et al. 2007

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“…) Therefore, the great interest has been recently focused on the natural foods, medicinal plants and phytoconstituents due to their well-known abilities to scavenge free radicals (i.e. antioxidant power) (Hou et al, 2003;Galvez et al, 2005;Kukic et al, 2006). Polyphenols possess many biological effects.…”

Section: +mentioning

confidence: 99%

Establishment of Enzyme Inhibitory Activities of Lovastatin, Isolated From Pleurotus ostreatus

Talkad

Das

Bhattacharjee

et al. 2015

Int J Appl Sci Biotechnol

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Enzyme inhibition is significant biological process to characterize the enzyme reaction, extraction of catalysis parameters in bio-industry and bioengineering. The enzyme inhibitors are low molecular weight chemical compounds. Inhibitor can modify one amino acid, or several side chain(s) required in enzyme catalytic activity. To protect enzyme catalytic site from any change, ligand binds with critical side chain in enzyme. Safely, chemical modification can be done to test inhibitor for any drug value. In the present day world they are known for culinary values due to their high-quality proteins, vitamins, fibers and many medicinal properties and accordingly they are called nutraceuticals. Overproduction of ROS can damage cellular biomolecules like nucleic acids, proteins, lipids, carbohydrates, proteins and enzymes resulting in several diseases. The chemical nature of the bioactive compounds present in this mushroom includes: polysaccharides, lipopolysaccharides, proteins, peptides, glycoproteins, lectins, lipids and their derivatives. We report the presence of Statins in fruiting bodies and fermentation processes from Pleurotus ostreatus. Lovastatin extracted from the Pleurotus ostreatus showed Anti-oxidant Activity when compared with Standard Quercetin, IC50 values of 3.5?g/ml and 7.5 ?g/ml respectively, amylase Assay the percentage inhibitions of 35% and 70 % with 1 and 3 mg/ml respectively. In Membrane Stability assay, the percentage inhibitions of 47.06% and 50%. Acid Phosphatase Assay: percentage inhibitions of 82.78% and 76.11% with 2mg/ml and 4mg/ml respectively. Pleurotus possess a good antioxidant and enzyme inhibitions for Alpha Amylase, Acid Phosphatase and membrane stability studies, hence as health promoter and environmental restorer is gaining more importance.Int J Appl Sci Biotechnol, Vol 3(3): 408-416

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Free radical-scavenging activity of Taiwanese native plants

Cited by 136 publications

References 22 publications

Inhibitory Effects of Citrus hassaku Extract and Its Flavanone Glycosides on Melanogenesis

Inhibitory Effects of Citrus hassaku Extract and Its Flavanone Glycosides on Melanogenesis

Fenóis totais e atividade antioxidante de cinco plantas medicinais

Establishment of Enzyme Inhibitory Activities of Lovastatin, Isolated From Pleurotus ostreatus

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