Free-radical scavenging activity of Bergia suffruticosa (Delile) Fenzl

Anandjiwala, Sheetal; Srinivasa, H.; Kalola, Jyoti; Rajani, M.

doi:10.1007/s11418-006-0017-7

Cited by 22 publications

(16 citation statements)

References 7 publications

(9 reference statements)

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“…3 Because of carcinogenicity of synthetic antioxidants, there is dearth for antioxidants from natural origin. 4 Natural antioxidants play a vital role in antioxidant defense mechanism in the biological system and acts as free radical scavenger.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of In-vitro Antioxidant Activity of Seagrasses: Signals for Potential Alternate Source

Danaraj

Subhashini

Raja

et al. 2016

FRA

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Background and Aim: Natural antioxidants have gained unique attention in recent years. Because of the carcinogenicity of synthetic compounds, there is a dearth for antioxidants from natural origin. Currently, seagrasses, the marine plants have gained attention for their secondary metabolites. Hence, the present study aims to examine in vitro antioxidant activity of both leaf and rhizome extracts of six seagrass species and has not yet been investigated. Methods: Crude methanolic extracts of leaf and rhizome obtained, were evaluated for total phenolic contents using Folin-Ciocalteaus method. Antioxidant potential of seagrass extracts were evaluated using total antioxidant activity, DPPH, FRAP, ABTS assay, H 2 O 2 and NO 2 scavenging assay and the phenolic compounds present in potent extracts were profiled by HPLC. Results: Maximum phenolic content and antioxidant activity was exhibited by leaf and rhizome extracts of C. rotundata followed by H. uninervis. Higher DPPH radical scavenging activity was found in leaf (78.84 ± 0.87) and rhizome extracts (75.480 ± 0.97) of C. rotundata and the lowest scavenging activity was found in the leaf and rhizome extracts of H. ovata (12.01 ± 0.63 and 5.769 ± 1.14). Among six species, C. rotundata exhibited higher radical scavenging activity containing the potential phenolic compounds. Conclusion: Present study portrays that leaf and rhizome extracts of C. rotundata acts as a potential source of antioxidant compounds with predominant presence of caffeic acid and ρ-coumaric acid that paves a way for the application of these compounds in both food and pharmaceutical industries as a multipotent antioxidant.

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Section: Introductionmentioning

confidence: 99%

Evaluation of In-vitro Antioxidant Activity of Seagrasses: Signals for Potential Alternate Source

Danaraj

Subhashini

Raja

et al. 2016

FRA

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“…Several reports of the synthesis of compounds with strong antioxidant properties have been published recently [4,5]. Because of the high carcinogenicity of synthetic antioxidants [6], there is preference to develop effective antioxidants of natural origin [7].…”

Section: Introductionmentioning

confidence: 99%

Thin layer chromatography analysis of antioxidant constituents of lichens from Antarctica

et al. 2008

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Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. Methanol-water (90:10 v/v) extracts of five polar lichen species--namely Stereocaulon alpinum Laurer (Stereocaulaceae); Ramalina terebrata Hook and Taylor (Ramalinaceae); Caloplaca sp. (Teloschistaceae); Lecanora sp. (Lecanoraceae); and Caloplaca regalis (Vain.)Zahlbr (Teloschistaceae) from King George Island (Antarctica)--were analyzed using thin layer chromatography (TLC) followed by a DPPH (2,2-diphenyl-1-picrylhydrazyl) spray technique. The experimental data showed that 33-50% of the major constituents of the test extracts were active antioxidants. Stereocaulon alpinum and R. terebrata showed a higher number (50%) of antioxidant constituents, although their activities were comparatively weak. The strength of antioxidant activity in terms of discoloration of DPPH was shown to be stronger by the constituents of S. alpinum, C. regalis and C. sp. In addition, phenolic content in these Antarctic lichen extracts was in the range of 17-47 mg/g, supporting the antioxidant data of TLC analysis. Thus, these results suggest that Antarctic lichen contains a variety of strong antioxidant constituents. Therefore, further study of the laboratory culture of lichen is warranted to investigate possible commercial production, followed by isolation and characterization of the active antioxidant agents, which can be used against various oxidative stress-related diseases.

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“…The free radicalscavenging activity of callus extracts was determined qualitatively using DPPH (Anandjiwala et al 2007), a molecule containing a stable free radical widely used to determine the free radical-scavenging ability of various samples. A 15-μL aliquot of callus extract (50 μg/mL) was carefully loaded on a 5×5-cm TLC layer (silica gel 60 F254; Merck), which was then developed with ethyl acetate:methanol:H 2 O (100:13:5:10, v/v).…”

Section: Methodsmentioning

confidence: 99%

Comparison of metabolite levels in callus of Tecoma stans (L.) Juss. ex Kunth. cultured in photoperiod and darkness

López-Laredo

Ramírez-Flores

Sepúlveda-Jiménez

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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Tecoma stans is a tropical plant from the Americas. Antioxidant activity and both phenolic compound and flavonoid total content were determined for callus tissue of T. stans cultured in either a set photoperiod or in darkness. Callus lines from three explant types (hypocotyls, stem, and leaf) were established on B5 culture medium supplemented with 0.5 μM 2,4-D and 5.0 μM kinetin. While leaf-derived callus grew slower under a 16-h photoperiod (specific growth rate, μ=0.179 d −1 , t D =3.9 d) than in darkness (μ=0.236 d −1 , t D =2.9 d), it accumulated the highest amount (p<0.05) of both phenolics (86.6±0.01 mg gallic acid equivalents/g) and flavonoids (339.6±0.06 mg catechin equivalents/g). Similarly, antioxidant activity was significantly higher (p<0.05) when callus was cultured in period light than when grown in extended darkness. Antioxidant activity measured with a 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based assay was 350.5±15.8 mmol Trolox/g extract for callus cultured under a defined photoperiod compared to 129.1±7.5 mmol Trolox/g extract from callus cultured in darkness. Content of phenolic compounds and flavonoids was in agreement with a better antioxidant power (EC 50 =450 μg extract/mg 1,1-diphenyl-2-picrylhydrazyl) and antiradical efficiency. Results of the present study show that calli of T. stans are a source of compounds with antioxidant activity that is favored by culture under a set photoperiod.

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Free-radical scavenging activity of Bergia suffruticosa (Delile) Fenzl

Cited by 22 publications

References 7 publications

Evaluation of In-vitro Antioxidant Activity of Seagrasses: Signals for Potential Alternate Source

Evaluation of In-vitro Antioxidant Activity of Seagrasses: Signals for Potential Alternate Source

Thin layer chromatography analysis of antioxidant constituents of lichens from Antarctica

Comparison of metabolite levels in callus of Tecoma stans (L.) Juss. ex Kunth. cultured in photoperiod and darkness

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